4',6-dimethoxyisoflavone-7-O-[beta]-D-glucopyranoside (wistin) is a peroxisome proliferator-activated receptor [gamma] (PPAR[gamma]) agonist that stimulates adipocyte differentiation

4',6-dimethoxyisoflavone-7-O-[beta]-D-glucopyranoside (wistin) is a peroxisome proliferator-activated receptor [gamma] (PPAR[gamma]) agonist that stimulates adipocyte differentiation

Peroxisome proliferator-activated receptor [gamma] (PPAR[gamma]) is a ligand-dependent transcription factor that directly activates the expression of adipocyte-specific genes, and is universally accepted as the master regulator for adipocyte differentiation. Using a PPAR[gamma] luciferase reporter a...

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Veröffentlicht in:Animal science journal 2016-11, Vol.87 (11), p.1347
Hauptverfasser: Sanada, Matoki, Hayashi, Ryuichi, Imai, Yoshimasa, Nakamura, Fumiya, Inoue, Tomoyo, Ohta, Shinji, Kawachi, Hiroyuki
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Gene expression
Genetic markers
Ligands
Transcription factors
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container_issue 11
container_start_page 1347
container_title Animal science journal
container_volume 87
creator Sanada, Matoki
Hayashi, Ryuichi
Imai, Yoshimasa
Nakamura, Fumiya
Inoue, Tomoyo
Ohta, Shinji
Kawachi, Hiroyuki
description Peroxisome proliferator-activated receptor [gamma] (PPAR[gamma]) is a ligand-dependent transcription factor that directly activates the expression of adipocyte-specific genes, and is universally accepted as the master regulator for adipocyte differentiation. Using a PPAR[gamma] luciferase reporter assay system, we showed that 4',6-dimethoxyisoflavone-7-O-[beta]-D-glucopyranoside (wistin) dose-dependently activates PPAR[gamma]. Treatment with wistin enhanced the marker of adipocyte differentiation, such as triglyceride accumulation in 3T3-L1 cells. Real-time quantitative polymerase chain reaction showed that wistin increased the expression of PPAR[gamma]2 messenger RNA. Moreover, the addition of wistin upregulated the expression of PPAR[gamma]-target genes, aP2 and adiponectin in 3T3-L1 cells. To our knowledge, wistin is the first isoflavonoid O-glycoside that exhibits PPAR[gamma] agonist activity.
doi_str_mv 10.1111/asj.12552
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subjects Gene expression
Genetic markers
Ligands
Transcription factors
title 4',6-dimethoxyisoflavone-7-O-[beta]-D-glucopyranoside (wistin) is a peroxisome proliferator-activated receptor [gamma] (PPAR[gamma]) agonist that stimulates adipocyte differentiation
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