Enhanced separation and analysis procedure reveals production of tri-acylated mannosylerythritol lipids by Pseudozyma aphidis
Mannosylerythritol lipids (MELs) are one of the most promising biosurfactants because of their high fermentation yields (>100 g l −1 ) and during the last two decades they have gained a lot of attention due to their interesting self-assembling properties and biological activities. In this study,...
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| Veröffentlicht in: | Journal of industrial microbiology & biotechnology 2016-11, Vol.43 (11), p.1537-1550 |
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| creator | Goossens, Eliane Wijnants, Marc Packet, Dirk Lemière, Filip |
| description | Mannosylerythritol lipids (MELs) are one of the most promising biosurfactants because of their high fermentation yields (>100 g l
−1
) and during the last two decades they have gained a lot of attention due to their interesting self-assembling properties and biological activities. In this study, MELs were produced by fed-batch bioreactor fermentation of rapeseed oil with
Pseudozyma aphidis
MUCL 27852. This high-level MEL-producing yeast secretes four conventional MEL structures, -A, -B, -C and -D, which differ in their degree of acetylation. During our research, unknown compounds synthesized by
P. aphidis
were detected by thin-layer chromatography. The unknown compounds were separated by flash chromatography and identified as tri-acylated MELs by high-performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS). The third fatty acid chain on the tri-acylated MELs was positioned on the primary alcohol of the erythritol moiety and comprised long-chain acids, mainly oleic and linoleic acid, which are not found in conventional di-acylated MELs. Furthermore, the LC–MS analysis time of conventional MELs was reduced to almost one-third by switching from HPLC–MS/MS to ultraperformance liquid chromatography tandem mass spectrometry (UPLC–MS/MS). Provided optimization of the fermentation yield,
P. aphidis
could be an interesting novel producer of tri-acylated MELs and, thereby expand the supply and applicability of biosurfactants. |
| doi_str_mv | 10.1007/s10295-016-1838-3 |
| format | Article |
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−1
) and during the last two decades they have gained a lot of attention due to their interesting self-assembling properties and biological activities. In this study, MELs were produced by fed-batch bioreactor fermentation of rapeseed oil with
Pseudozyma aphidis
MUCL 27852. This high-level MEL-producing yeast secretes four conventional MEL structures, -A, -B, -C and -D, which differ in their degree of acetylation. During our research, unknown compounds synthesized by
P. aphidis
were detected by thin-layer chromatography. The unknown compounds were separated by flash chromatography and identified as tri-acylated MELs by high-performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS). The third fatty acid chain on the tri-acylated MELs was positioned on the primary alcohol of the erythritol moiety and comprised long-chain acids, mainly oleic and linoleic acid, which are not found in conventional di-acylated MELs. Furthermore, the LC–MS analysis time of conventional MELs was reduced to almost one-third by switching from HPLC–MS/MS to ultraperformance liquid chromatography tandem mass spectrometry (UPLC–MS/MS). Provided optimization of the fermentation yield,
P. aphidis
could be an interesting novel producer of tri-acylated MELs and, thereby expand the supply and applicability of biosurfactants.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1007/s10295-016-1838-3</identifier><identifier>PMID: 27659961</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acetylation ; Analysis ; Biochemistry ; Bioinformatics ; Biomedical and Life Sciences ; Bioreactors ; Bioremediation ; Biosynthesis ; Biotechnology ; Canola Oil - metabolism ; Cell Culture and Bioengineering - Original Paper ; Chemicals ; Chromatography ; Chromatography, High Pressure Liquid ; Chromatography, Thin Layer ; Enhanced oil recovery ; Fatty acids ; Fatty Acids - analysis ; Fermentation ; Genetic Engineering ; Glucose ; Glycolipids - chemistry ; Glycolipids - isolation & purification ; Glycolipids - metabolism ; Inorganic Chemistry ; Life Sciences ; Lipids ; Liquid chromatography ; Mass spectrometry ; Microbiology ; Microorganisms ; Molecular weight ; Production capacity ; Rapeseed oil ; Raw materials ; Scientific imaging ; Studies ; Surface-Active Agents - chemistry ; Surface-Active Agents - isolation & purification ; Surface-Active Agents - metabolism ; Surfactants ; Tandem Mass Spectrometry ; Thin layer chromatography ; Ustilaginales - metabolism ; Yeasts</subject><ispartof>Journal of industrial microbiology & biotechnology, 2016-11, Vol.43 (11), p.1537-1550</ispartof><rights>Society for Industrial Microbiology and Biotechnology 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-9341994fe3728f65e201e31ae9c58f6e077faa2e310a13728ce83255b6c428443</citedby><cites>FETCH-LOGICAL-c376t-9341994fe3728f65e201e31ae9c58f6e077faa2e310a13728ce83255b6c428443</cites><orcidid>0000-0001-6679-2520</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10295-016-1838-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10295-016-1838-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27659961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goossens, Eliane</creatorcontrib><creatorcontrib>Wijnants, Marc</creatorcontrib><creatorcontrib>Packet, Dirk</creatorcontrib><creatorcontrib>Lemière, Filip</creatorcontrib><title>Enhanced separation and analysis procedure reveals production of tri-acylated mannosylerythritol lipids by Pseudozyma aphidis</title><title>Journal of industrial microbiology & biotechnology</title><addtitle>J Ind Microbiol Biotechnol</addtitle><addtitle>J Ind Microbiol Biotechnol</addtitle><description>Mannosylerythritol lipids (MELs) are one of the most promising biosurfactants because of their high fermentation yields (>100 g l
−1
) and during the last two decades they have gained a lot of attention due to their interesting self-assembling properties and biological activities. In this study, MELs were produced by fed-batch bioreactor fermentation of rapeseed oil with
Pseudozyma aphidis
MUCL 27852. This high-level MEL-producing yeast secretes four conventional MEL structures, -A, -B, -C and -D, which differ in their degree of acetylation. During our research, unknown compounds synthesized by
P. aphidis
were detected by thin-layer chromatography. The unknown compounds were separated by flash chromatography and identified as tri-acylated MELs by high-performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS). The third fatty acid chain on the tri-acylated MELs was positioned on the primary alcohol of the erythritol moiety and comprised long-chain acids, mainly oleic and linoleic acid, which are not found in conventional di-acylated MELs. Furthermore, the LC–MS analysis time of conventional MELs was reduced to almost one-third by switching from HPLC–MS/MS to ultraperformance liquid chromatography tandem mass spectrometry (UPLC–MS/MS). Provided optimization of the fermentation yield,
P. aphidis
could be an interesting novel producer of tri-acylated MELs and, thereby expand the supply and applicability of biosurfactants.</description><subject>Acetylation</subject><subject>Analysis</subject><subject>Biochemistry</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Bioremediation</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Canola Oil - metabolism</subject><subject>Cell Culture and Bioengineering - Original Paper</subject><subject>Chemicals</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Thin Layer</subject><subject>Enhanced oil recovery</subject><subject>Fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>Fermentation</subject><subject>Genetic Engineering</subject><subject>Glucose</subject><subject>Glycolipids - chemistry</subject><subject>Glycolipids - isolation & 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microbiology & biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goossens, Eliane</au><au>Wijnants, Marc</au><au>Packet, Dirk</au><au>Lemière, Filip</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced separation and analysis procedure reveals production of tri-acylated mannosylerythritol lipids by Pseudozyma aphidis</atitle><jtitle>Journal of industrial microbiology & biotechnology</jtitle><stitle>J Ind Microbiol Biotechnol</stitle><addtitle>J Ind Microbiol Biotechnol</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>43</volume><issue>11</issue><spage>1537</spage><epage>1550</epage><pages>1537-1550</pages><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>Mannosylerythritol lipids (MELs) are one of the most promising biosurfactants because of their high fermentation yields (>100 g l
−1
) and during the last two decades they have gained a lot of attention due to their interesting self-assembling properties and biological activities. In this study, MELs were produced by fed-batch bioreactor fermentation of rapeseed oil with
Pseudozyma aphidis
MUCL 27852. This high-level MEL-producing yeast secretes four conventional MEL structures, -A, -B, -C and -D, which differ in their degree of acetylation. During our research, unknown compounds synthesized by
P. aphidis
were detected by thin-layer chromatography. The unknown compounds were separated by flash chromatography and identified as tri-acylated MELs by high-performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS). The third fatty acid chain on the tri-acylated MELs was positioned on the primary alcohol of the erythritol moiety and comprised long-chain acids, mainly oleic and linoleic acid, which are not found in conventional di-acylated MELs. Furthermore, the LC–MS analysis time of conventional MELs was reduced to almost one-third by switching from HPLC–MS/MS to ultraperformance liquid chromatography tandem mass spectrometry (UPLC–MS/MS). Provided optimization of the fermentation yield,
P. aphidis
could be an interesting novel producer of tri-acylated MELs and, thereby expand the supply and applicability of biosurfactants.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27659961</pmid><doi>10.1007/s10295-016-1838-3</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-6679-2520</orcidid></addata></record> |
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| ispartof | Journal of industrial microbiology & biotechnology, 2016-11, Vol.43 (11), p.1537-1550 |
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| source | Oxford Journals Open Access Collection; MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Acetylation Analysis Biochemistry Bioinformatics Biomedical and Life Sciences Bioreactors Bioremediation Biosynthesis Biotechnology Canola Oil - metabolism Cell Culture and Bioengineering - Original Paper Chemicals Chromatography Chromatography, High Pressure Liquid Chromatography, Thin Layer Enhanced oil recovery Fatty acids Fatty Acids - analysis Fermentation Genetic Engineering Glucose Glycolipids - chemistry Glycolipids - isolation & purification Glycolipids - metabolism Inorganic Chemistry Life Sciences Lipids Liquid chromatography Mass spectrometry Microbiology Microorganisms Molecular weight Production capacity Rapeseed oil Raw materials Scientific imaging Studies Surface-Active Agents - chemistry Surface-Active Agents - isolation & purification Surface-Active Agents - metabolism Surfactants Tandem Mass Spectrometry Thin layer chromatography Ustilaginales - metabolism Yeasts |
| title | Enhanced separation and analysis procedure reveals production of tri-acylated mannosylerythritol lipids by Pseudozyma aphidis |
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