Novel Genes Encoding Hexadecanoic Acid [Delta]6-Desaturase Activity in a Rhodococcus sp
cis-6-Hexadecenoic acid, a major component of human sebaceous lipids, is involved in the defense mechanism against Staphylococcus aureus infection in healthy skin and closely related to atopic dermatitis. Previously, Koike et al. (Biosci Biotechnol Biochem 64:1064-1066, 2000 ) reported that a mutant...
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| Veröffentlicht in: | Current microbiology 2016-11, Vol.73 (5), p.646 |
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| creator | Araki, Hiroyuki Hagihara, Hiroshi Takigawa, Hirofumi Tsujino, Yukiharu Ozaki, Katsuya |
| description | cis-6-Hexadecenoic acid, a major component of human sebaceous lipids, is involved in the defense mechanism against Staphylococcus aureus infection in healthy skin and closely related to atopic dermatitis. Previously, Koike et al. (Biosci Biotechnol Biochem 64:1064-1066, 2000 ) reported that a mutant strain of Rhodococcus sp. produced cis-6-hexadecenoate derivatives from palmitate alkyl esters. From the mutant Rhodococcus strain, we identified and sequenced two open reading frames present in an amplified 5.7-kb region; these open reading frames encoded tandemly repeated [Delta]6-desaturase-like genes, Rdes1 and Rdes2. A phylogenetic tree indicated that Rdes1 and Rdes2 were different from previously known [Delta]6-desaturase genes, and that they formed a new cluster. Rdes1 and Rdes2 were each introduced into vectors and then expressed separately in Escherichia coli, and the fatty acid composition of the transformed cells was analyzed by gas chromatography and mass spectrometry. The amount of cis-6-hexadecenoic acid was significantly higher in Rdes1- or Rdes2-transformed E. coli cells (twofold and threefold, respectively) than in vector-only control cells. These results showed that cis-6-hexadecenoic acid was produced in E. coli cells by the rhodococcal [Delta]6-desaturase-like proteins. |
| doi_str_mv | 10.1007/s00284-016-1106-9 |
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Previously, Koike et al. (Biosci Biotechnol Biochem 64:1064-1066, 2000 ) reported that a mutant strain of Rhodococcus sp. produced cis-6-hexadecenoate derivatives from palmitate alkyl esters. From the mutant Rhodococcus strain, we identified and sequenced two open reading frames present in an amplified 5.7-kb region; these open reading frames encoded tandemly repeated [Delta]6-desaturase-like genes, Rdes1 and Rdes2. A phylogenetic tree indicated that Rdes1 and Rdes2 were different from previously known [Delta]6-desaturase genes, and that they formed a new cluster. Rdes1 and Rdes2 were each introduced into vectors and then expressed separately in Escherichia coli, and the fatty acid composition of the transformed cells was analyzed by gas chromatography and mass spectrometry. The amount of cis-6-hexadecenoic acid was significantly higher in Rdes1- or Rdes2-transformed E. coli cells (twofold and threefold, respectively) than in vector-only control cells. These results showed that cis-6-hexadecenoic acid was produced in E. coli cells by the rhodococcal [Delta]6-desaturase-like proteins.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-016-1106-9</identifier><language>eng</language><publisher>New York: Springer Nature B.V</publisher><subject>Bacteria ; Dermatitis ; E coli ; Esters ; Fatty acids ; Gas chromatography ; Lipids ; Mass spectrometry ; Mutation ; Phylogenetics</subject><ispartof>Current microbiology, 2016-11, Vol.73 (5), p.646</ispartof><rights>Springer Science+Business Media New York 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Araki, Hiroyuki</creatorcontrib><creatorcontrib>Hagihara, Hiroshi</creatorcontrib><creatorcontrib>Takigawa, Hirofumi</creatorcontrib><creatorcontrib>Tsujino, Yukiharu</creatorcontrib><creatorcontrib>Ozaki, Katsuya</creatorcontrib><title>Novel Genes Encoding Hexadecanoic Acid [Delta]6-Desaturase Activity in a Rhodococcus sp</title><title>Current microbiology</title><description>cis-6-Hexadecenoic acid, a major component of human sebaceous lipids, is involved in the defense mechanism against Staphylococcus aureus infection in healthy skin and closely related to atopic dermatitis. 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These results showed that cis-6-hexadecenoic acid was produced in E. coli cells by the rhodococcal [Delta]6-desaturase-like proteins.</description><subject>Bacteria</subject><subject>Dermatitis</subject><subject>E coli</subject><subject>Esters</subject><subject>Fatty acids</subject><subject>Gas chromatography</subject><subject>Lipids</subject><subject>Mass spectrometry</subject><subject>Mutation</subject><subject>Phylogenetics</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNisFOwkAQQDdGE4vyAdwm8bwy0y21PRpAOHEwJh6MIZvtAEuaXexsCf69PfAB5h3e4T2lJoTPhPgyFcS8KjRSqYmw1PWNyqgwuca6pluVoSmMrsoZ3auRyBGR8hopU5-beOYWVhxYYBlcbHzYw5ovtmFnQ_QOXp1v4GvBbbLfpV6w2NR3VngIyZ99-gUfwML7ITbRRed6ATk9qrudbYXHVz-op7flx3ytT1386VnS9hj7LgxpS1VuqgFD5n_XH5HcRxc</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Araki, Hiroyuki</creator><creator>Hagihara, Hiroshi</creator><creator>Takigawa, Hirofumi</creator><creator>Tsujino, Yukiharu</creator><creator>Ozaki, Katsuya</creator><general>Springer Nature B.V</general><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20161101</creationdate><title>Novel Genes Encoding Hexadecanoic Acid [Delta]6-Desaturase Activity in a Rhodococcus sp</title><author>Araki, Hiroyuki ; 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| subjects | Bacteria Dermatitis E coli Esters Fatty acids Gas chromatography Lipids Mass spectrometry Mutation Phylogenetics |
| title | Novel Genes Encoding Hexadecanoic Acid [Delta]6-Desaturase Activity in a Rhodococcus sp |
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