Microwave-Induced Structural and Functional Injury of Hippocampal and PC12 Cells Is Accompanied by Abnormal Changes in the NMDAR-PSD95-CaMKII Pathway
Recent studies have highlighted the important role of the postsynaptic NMDAR-PSD95-CaMKII pathway for synaptic transmission and related neuronal injury. Here, we tested changes in the components of this pathway upon microwave-induced neuronal structure and function impairments. Ultrastructural and f...
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| Veröffentlicht in: | Pathobiology (Basel) 2015-01, Vol.82 (5), p.181-194 |
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| creator | Wang, Li-Feng Wei, Li Qiao, Si-Mo Gao, Xiao-Na Gao, Ya-Bing Wang, Shui-Ming Zhao, Li Dong, Ji Xu, Xin-Ping Zhou, Hong-Mei Hu, Xiang-Jun Peng, Rui-Yun |
| description | Recent studies have highlighted the important role of the postsynaptic NMDAR-PSD95-CaMKII pathway for synaptic transmission and related neuronal injury. Here, we tested changes in the components of this pathway upon microwave-induced neuronal structure and function impairments. Ultrastructural and functional changes were induced in hippocampal neurons of rats and in PC12 cells exposed to microwave radiation. We detected abnormal protein and mRNA expression, as well as posttranslational modifications in the NMDAR-PSD95-CaMKII pathway and its associated components, such as synapsin I, following microwave radiation exposure of rats and PC12 cells. Thus, microwave radiation may induce neuronal injury via changes in the molecular organization of postsynaptic density and modulation of the biochemical cascade that potentiates synaptic transmission. |
| doi_str_mv | 10.1159/000398803 |
| format | Article |
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Here, we tested changes in the components of this pathway upon microwave-induced neuronal structure and function impairments. Ultrastructural and functional changes were induced in hippocampal neurons of rats and in PC12 cells exposed to microwave radiation. We detected abnormal protein and mRNA expression, as well as posttranslational modifications in the NMDAR-PSD95-CaMKII pathway and its associated components, such as synapsin I, following microwave radiation exposure of rats and PC12 cells. Thus, microwave radiation may induce neuronal injury via changes in the molecular organization of postsynaptic density and modulation of the biochemical cascade that potentiates synaptic transmission.</description><identifier>ISSN: 1015-2008</identifier><identifier>EISSN: 1423-0291</identifier><identifier>DOI: 10.1159/000398803</identifier><identifier>PMID: 26337368</identifier><identifier>CODEN: PATHEF</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Animals ; Biochemistry ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism ; Disks Large Homolog 4 Protein ; Hippocampus - chemistry ; Hippocampus - cytology ; Hippocampus - radiation effects ; Hippocampus - ultrastructure ; Intracellular Signaling Peptides and Proteins - genetics ; Intracellular Signaling Peptides and Proteins - metabolism ; Male ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Microwave radiation ; Microwaves - adverse effects ; Neurons ; Neurons - metabolism ; Neurons - radiation effects ; Neurons - ultrastructure ; Neurotransmitters ; Original Paper ; PC12 Cells ; Post-Synaptic Density - radiation effects ; Protein Processing, Post-Translational - genetics ; Protein Processing, Post-Translational - radiation effects ; Radiation ; Rats ; Receptors, N-Methyl-D-Aspartate - metabolism ; Receptors, N-Methyl-D-Aspartate - physiology ; Signal Transduction ; Synaptic Transmission - radiation effects</subject><ispartof>Pathobiology (Basel), 2015-01, Vol.82 (5), p.181-194</ispartof><rights>2015 S. 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Thus, microwave radiation may induce neuronal injury via changes in the molecular organization of postsynaptic density and modulation of the biochemical cascade that potentiates synaptic transmission.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics</subject><subject>Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism</subject><subject>Disks Large Homolog 4 Protein</subject><subject>Hippocampus - chemistry</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - radiation effects</subject><subject>Hippocampus - ultrastructure</subject><subject>Intracellular Signaling Peptides and Proteins - genetics</subject><subject>Intracellular Signaling Peptides and Proteins - metabolism</subject><subject>Male</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Microwave radiation</subject><subject>Microwaves - adverse effects</subject><subject>Neurons</subject><subject>Neurons - metabolism</subject><subject>Neurons - radiation effects</subject><subject>Neurons - ultrastructure</subject><subject>Neurotransmitters</subject><subject>Original Paper</subject><subject>PC12 Cells</subject><subject>Post-Synaptic Density - radiation effects</subject><subject>Protein Processing, Post-Translational - genetics</subject><subject>Protein Processing, Post-Translational - radiation effects</subject><subject>Radiation</subject><subject>Rats</subject><subject>Receptors, N-Methyl-D-Aspartate - metabolism</subject><subject>Receptors, N-Methyl-D-Aspartate - physiology</subject><subject>Signal Transduction</subject><subject>Synaptic Transmission - radiation effects</subject><issn>1015-2008</issn><issn>1423-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0U1v1DAQBmALgegHHLgjZIkLPQQ8dpzExyilNGoXVrScI8dxulk2drDjVvtD-L812mUrIU625UfvaGYQegPkIwAXnwghTBQFYc_QMaSUJYQKeB7vBHhCCSmO0In368gKkpGX6IhmjOUsK47R78WgnH2Q9zqpTReU7vDN7IKag5MbLE2HL4JR82BNfNZmHdwW2x5fDtNklRynPVpWQHGlNxuPa49LpWz8MkNMa7e4bI11Y5TVSpo77fFg8LzS-OvivPyeLG_OBU8qubiqa7yU8-pBbl-hF73ceP16f56iHxefb6vL5Prbl7oqrxPFRD4noLjq2kKlHARllPJWECEyQWXXSplmOchccIBcFq3IOCWacGAkBcn6XnWEnaIPu9zJ2V9B-7kZB69iG9JoG3wDOWSUcyjySN__Q9c2uDiVqAqgNE8jjOpsp-JQvXe6byY3jNJtGyDNn101h11F-26fGNpRdwf5dzlPJX9Kd6fdASzL211EM3V9VG__q_ZVHgGq96Bv</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Wang, Li-Feng</creator><creator>Wei, Li</creator><creator>Qiao, Si-Mo</creator><creator>Gao, Xiao-Na</creator><creator>Gao, Ya-Bing</creator><creator>Wang, Shui-Ming</creator><creator>Zhao, Li</creator><creator>Dong, Ji</creator><creator>Xu, Xin-Ping</creator><creator>Zhou, Hong-Mei</creator><creator>Hu, Xiang-Jun</creator><creator>Peng, Rui-Yun</creator><general>S. 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Here, we tested changes in the components of this pathway upon microwave-induced neuronal structure and function impairments. Ultrastructural and functional changes were induced in hippocampal neurons of rats and in PC12 cells exposed to microwave radiation. We detected abnormal protein and mRNA expression, as well as posttranslational modifications in the NMDAR-PSD95-CaMKII pathway and its associated components, such as synapsin I, following microwave radiation exposure of rats and PC12 cells. Thus, microwave radiation may induce neuronal injury via changes in the molecular organization of postsynaptic density and modulation of the biochemical cascade that potentiates synaptic transmission.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>26337368</pmid><doi>10.1159/000398803</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0003-1895-8754</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1015-2008 |
| ispartof | Pathobiology (Basel), 2015-01, Vol.82 (5), p.181-194 |
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| subjects | Animals Biochemistry Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism Disks Large Homolog 4 Protein Hippocampus - chemistry Hippocampus - cytology Hippocampus - radiation effects Hippocampus - ultrastructure Intracellular Signaling Peptides and Proteins - genetics Intracellular Signaling Peptides and Proteins - metabolism Male Membrane Proteins - genetics Membrane Proteins - metabolism Microwave radiation Microwaves - adverse effects Neurons Neurons - metabolism Neurons - radiation effects Neurons - ultrastructure Neurotransmitters Original Paper PC12 Cells Post-Synaptic Density - radiation effects Protein Processing, Post-Translational - genetics Protein Processing, Post-Translational - radiation effects Radiation Rats Receptors, N-Methyl-D-Aspartate - metabolism Receptors, N-Methyl-D-Aspartate - physiology Signal Transduction Synaptic Transmission - radiation effects |
| title | Microwave-Induced Structural and Functional Injury of Hippocampal and PC12 Cells Is Accompanied by Abnormal Changes in the NMDAR-PSD95-CaMKII Pathway |
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