108. In Vitro Down-Regulation of Transgene Expression with Cre/loxP Mediated Recombination
Safe and durable transgene expression for the treatment of hepatic diseases has been reported utilizing several different viral vector systems. Following gene transfer, over-expression of some gene products may be undesirable. To this end, we prepared a recombinant AAV reporter vector that would be...
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| Veröffentlicht in: | Molecular therapy 2004-05, Vol.9 (S1), p.S42 |
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| creator | Ii, J Lawrence Merritt Daniels, Jan Schowalter, David B |
| description | Safe and durable transgene expression for the treatment of hepatic diseases has been reported utilizing several different viral vector systems. Following gene transfer, over-expression of some gene products may be undesirable. To this end, we prepared a recombinant AAV reporter vector that would be susceptible to molecular recombination that would terminate transgene expression. The AAV beta-galactosidase (LacZ) reporter vector (pAAV-LacZ) was modified to include loxP sites at two unique restriction enzyme sites flanking the LacZ expression cassette (pAAV-loxPLacZ). Sequential treatment of cultured human hepatoma cells (SKHep1) transfected with pAAV-loxPLacZ, followed by infection with a first generation adenovirus expressing the Cre recombinase (Ad/Cre) resulted in a 67% reduction of LacZ expression compared to control cells. Additional studies towards optimization of this molecular recombination as a form of reversible transgene expression are currently in progress. These in vitro studies represent an important step in the development of a safe, durable and reversible AAV-gene delivery system. |
| doi_str_mv | 10.1016/j.ymthe.2004.06.045 |
| format | Article |
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| ispartof | Molecular therapy, 2004-05, Vol.9 (S1), p.S42 |
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| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ProQuest Central UK/Ireland; Alma/SFX Local Collection |
| subjects | Adenoviruses Cytokeratin Cytomegalovirus Gene therapy Kinases Medicine Proteins Smooth muscle Transcription factors Vectors (Biology) |
| title | 108. In Vitro Down-Regulation of Transgene Expression with Cre/loxP Mediated Recombination |
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