Adaptation and Validation of E-Probe Diagnostic Nucleic Acid Analysis for Detection of Escherichia coli O157:H7 in Metagenomic Data from Complex Food Matrices

The Centers for Disease Control and Prevention recently emphasized the need for enhanced technologies to use in investigations of outbreaks of foodborne illnesses. To address this need, e-probe diagnostic nucleic acid analysis (EDNA) was adapted and validated as a tool for the rapid, effective ident...

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Veröffentlicht in:	Journal of food protection 2016-04, Vol.79 (4), p.574-581
Hauptverfasser:	Blagden, Trenna, Schneider, William, Melcher, Ulrich, Daniels, Jon, Fletcher, Jacqueline
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Adaptation Agriculture Alfalfa Bioinformatics Colleges & universities Data analysis Disease control Disease prevention DNA, Bacterial - genetics E coli Escherichia coli O157 - classification Escherichia coli O157 - genetics Escherichia coli O157 - isolation & purification Experiments Food Food Contamination - analysis Food matrix Food safety Foodborne diseases Foodborne Diseases - microbiology Genomes High-Throughput Nucleotide Sequencing Humans Medicago sativa - microbiology Metagenomics - methods Methods Nucleic acids Outbreaks Pathogens Pipelines Probes Queries Seeds United States Vegetables - microbiology
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