AB0160 Epigenetic Studies in Maternally versus Paternally Transmitted Psoriatic Disease

Background Epidemiological studies have noted excess paternal transmission in psoriasis and psoriatic arthritis. To date, there has been no molecular explanation to account for this observation. Differential methylation patterns have long been implicated as a potential mechanism to account for excess paternal transmission. Objectives In this pilot study, we investigated the differential methylation pattern among paternally and maternally transmitted PsA. Methods Twenty-four (24) patients with maternally transmitted PsA were compared with 24 paternally transmitted PsA cases. For maternally transmitted PsA, the proband's mother had either psoriasis or PsA, and for paternally transmitted PsA, the proband's father had either psoriasis or PsA. Genome-wide DNA methylation profiling was performed on all these 48 samples by using Illumina HumanMethylation450k Beadchip, which measures up ∼480,000 different CpG sites per sample and covers 96% of RefSeq genes. The methylation level at each CpG site was measured by β values varying from 0 (no methylation) to 1 (100% methylation). Results Maternally transmitted PsA cases were predominantly females (19/24) with mean age of onset of PsA at 30.6 years. For paternally transmitted PsA, there were slightly more females (13/24) and age of onset of PsA was 22.2 years. Methylation data were normalized using BMIQ method and no batch effects were detected by PCA analysis. Methylation analysis was performed on 382,024 of the 485,512 CpG sites after filtering and revealed 90 significant CpG sites (p