Purification of [alpha]-glucosidase from mouse intestine by countercurrent chromatography coupled with a reverse micelle solvent system

Countercurrent chromatography coupled with a reverse micelle solvent was applied to separate [alpha]-glucosidase, which is stable at pH 6.0-8.8, 15-50°C. The separation conditions are as follows: stationary phase: pH 4.0 Tris-HCl buffer phase containing 50 mM Tris-HCl and 50 mM KCl; mobile phase A:...

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Veröffentlicht in:	Journal of separation science 2016-02, Vol.39 (4), p.703
Hauptverfasser:	He, Kai, Zou, Zongyao, Hu, Yinran, Yang, Yong, Xiao, Yubo, Gao, Pincao, Li, Xuegang, Ye, Xiaoli
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography Enzymes Proteins Solvents
Online-Zugang:	Volltext
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