Utility of Immunoproteomics in Soybean Allergy

Utility of Immunoproteomics in Soybean Allergy

PVDF membranes were stained with Direct Blue 71 and spots coincident with IgE binding were digested with lysyl endopeptidase and analyzed using TripleTOF 6600 liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).

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Bibliographische Detailangaben
Veröffentlicht in:Journal of allergy and clinical immunology 2016-02, Vol.137 (2), p.AB139-AB139
Hauptverfasser: Shimojo, Naoshi, PhD, Nakamura, Masashi, Sato, Nayu, Sano, Akiyo, MD, Kobayashi, Tsukane, MD, PhD, Yagami, Akiko, MD, PhD, Kojima, Atsushi, Matsunaga, Kayoko, MD, PhD
Format: Artikel
Sprache:eng
Schlagworte:
Allergies
Allergy and Immunology
Proteins
Soybeans
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Beschreibung
Zusammenfassung:PVDF membranes were stained with Direct Blue 71 and spots coincident with IgE binding were digested with lysyl endopeptidase and analyzed using TripleTOF 6600 liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2015.12.587