RAPD Profiling of Three Japanese Drosera Species
RAPD analysis was carried out using 1200 decamer random sequences to clarify the whole fingerprint of DNA fragment appearance patterns among three Japanese Drosera species: D. tokaiensis, D. rotundifolia and D. spatulata. The RAPD analysis results showed that D. tokaiensis had not only magnificent b...
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Veröffentlicht in: | CYTOLOGIA 2015/12/25, Vol.80(4), pp.393-398 |
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description | RAPD analysis was carried out using 1200 decamer random sequences to clarify the whole fingerprint of DNA fragment appearance patterns among three Japanese Drosera species: D. tokaiensis, D. rotundifolia and D. spatulata. The RAPD analysis results showed that D. tokaiensis had not only magnificent bands common to other two species, but also many specific bands, although D. tokaiensis is of amphiploidal origin between D. rotundifolia and D. spatulata. The specific bands of D. tokaiensis might generate after the speciation or amphiduplication. Therefore, the relationship of genome compositions among the three species suggested that RAPD fragments were preferentially amplified from 20 middle-sized chromosomes in D. rotundifolia and D. tokaiensis. Thus, the chromosome information and the RAPD profiling suggested that alloploidal genome formation during Drosera speciation might obtain new beneficial genetic characters to survive and adapt to certain environments. |
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Thus, the chromosome information and the RAPD profiling suggested that alloploidal genome formation during Drosera speciation might obtain new beneficial genetic characters to survive and adapt to certain environments.</description><subject>Cytogenetics</subject><subject>Drosera rotundifolia</subject><subject>Drosera spatulata</subject><subject>Drosera tokaiensis</subject><subject>Genome</subject><subject>RAPD</subject><issn>0011-4545</issn><issn>1348-7019</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNpVkD1PwzAQhi0EElXpzhiJOeUc23E8Vi2fqkQFZbYc99ymCnGw06H_nqBURUw33Pu8d3oIuaUwpQKKe3vsfO23lZkWMGWKXZARZbxIJVB1SUYAlKZccHFNJjFWJUCWS1AyGxF4n60WySp4V9VVs028S9a7gJi8mtY0GDFZBB8xmOSjRVthvCFXztQRJ6c5Jp-PD-v5c7p8e3qZz5apFRy6tLRKMJOZPOOyZM4VHLmUbiNpLhiVnOfOcFRWKieyDUhrXUFBljbPenDj2JjcDb1t8N8HjJ3e-0No-pOaSsGYUFyqPgVDyvZfxoBOt6H6MuGoKehfNfqsRhegezU9Mh-QfezMFs-ACV1la_wP8BP1t92ZoLFhP1_xcGA</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Tungkajiwangkoon, Santhita</creator><creator>Inagaki, Ayato</creator><creator>Shirakawa, Junichi</creator><creator>Hoshi, Yoshikazu</creator><general>Japan Mendel Society, International Society of Cytology</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>2015</creationdate><title>RAPD Profiling of Three Japanese Drosera Species</title><author>Tungkajiwangkoon, Santhita ; Inagaki, Ayato ; Shirakawa, Junichi ; Hoshi, Yoshikazu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-bc953a2a6247b3ff84e477fd7165317446fa4e9c79f52d07ccf8107bc62953df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cytogenetics</topic><topic>Drosera rotundifolia</topic><topic>Drosera spatulata</topic><topic>Drosera tokaiensis</topic><topic>Genome</topic><topic>RAPD</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tungkajiwangkoon, Santhita</creatorcontrib><creatorcontrib>Inagaki, Ayato</creatorcontrib><creatorcontrib>Shirakawa, Junichi</creatorcontrib><creatorcontrib>Hoshi, Yoshikazu</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>CYTOLOGIA</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tungkajiwangkoon, Santhita</au><au>Inagaki, Ayato</au><au>Shirakawa, Junichi</au><au>Hoshi, Yoshikazu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RAPD Profiling of Three Japanese Drosera Species</atitle><jtitle>CYTOLOGIA</jtitle><date>2015</date><risdate>2015</risdate><volume>80</volume><issue>4</issue><spage>393</spage><epage>398</epage><pages>393-398</pages><issn>0011-4545</issn><eissn>1348-7019</eissn><abstract>RAPD analysis was carried out using 1200 decamer random sequences to clarify the whole fingerprint of DNA fragment appearance patterns among three Japanese Drosera species: D. tokaiensis, D. rotundifolia and D. spatulata. The RAPD analysis results showed that D. tokaiensis had not only magnificent bands common to other two species, but also many specific bands, although D. tokaiensis is of amphiploidal origin between D. rotundifolia and D. spatulata. The specific bands of D. tokaiensis might generate after the speciation or amphiduplication. Therefore, the relationship of genome compositions among the three species suggested that RAPD fragments were preferentially amplified from 20 middle-sized chromosomes in D. rotundifolia and D. tokaiensis. Thus, the chromosome information and the RAPD profiling suggested that alloploidal genome formation during Drosera speciation might obtain new beneficial genetic characters to survive and adapt to certain environments.</abstract><cop>Tokyo</cop><pub>Japan Mendel Society, International Society of Cytology</pub><doi>10.1508/cytologia.80.393</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Cytogenetics Drosera rotundifolia Drosera spatulata Drosera tokaiensis Genome RAPD |
title | RAPD Profiling of Three Japanese Drosera Species |
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