Studying Cooperative Ligand Binding in the Undergraduate Biochemistry Laboratory: Oxygen–Hemoglobin Dissociation Revisited

Studying Cooperative Ligand Binding in the Undergraduate Biochemistry Laboratory: Oxygen–Hemoglobin Dissociation Revisited

The interaction between oxygen and hemoglobin is a classic example of a cooperative ligand-binding process. This gaseous-ligand binding process may be studied in the undergraduate laboratory with a variable-pressure cuvette apparatus. An updated method is described to assemble a variable-pressure cu...

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Veröffentlicht in:Journal of chemical education 2015-12, Vol.92 (12), p.2173-2175
Hauptverfasser: Young, Megan K. M, Gries, Theodore J
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Sprache:eng
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Binding
Chemistry
Hemoglobin
Ligands
Organic chemistry
Oxygen
Pressure
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container_title Journal of chemical education
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creator Young, Megan K. M
Gries, Theodore J
description The interaction between oxygen and hemoglobin is a classic example of a cooperative ligand-binding process. This gaseous-ligand binding process may be studied in the undergraduate laboratory with a variable-pressure cuvette apparatus. An updated method is described to assemble a variable-pressure cuvette apparatus composed of inexpensive supplies and equipment commonly found in the chemistry laboratory.
doi_str_mv 10.1021/acs.jchemed.5b00374
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1938-1328
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source American Chemical Society Journals
subjects Binding
Chemistry
Hemoglobin
Ligands
Organic chemistry
Oxygen
Pressure
title Studying Cooperative Ligand Binding in the Undergraduate Biochemistry Laboratory: Oxygen–Hemoglobin Dissociation Revisited
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