Semi‐automated reconstruction of inflammatory infiltration in infectious keratitis

Purpose To perform reconstruction of inflammatory cells infiltration in infectious keratitis. Methods First we performed in vivo confocal microscopy (HRT III, Rostock Cornea Module) in 118 patients diagnosed for the infectious keratitis (45 viral, 40 bacterial, 23 fungal, 10 amoebal). Inflammatory c...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Acta ophthalmologica (Oxford, England) England), 2015-10, Vol.93 (S255), p.n/a
Hauptverfasser: Smedowski, A., Tarnawska, D., Wylegala, E.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page n/a
container_issue S255
container_start_page
container_title Acta ophthalmologica (Oxford, England)
container_volume 93
creator Smedowski, A.
Tarnawska, D.
Wylegala, E.
description Purpose To perform reconstruction of inflammatory cells infiltration in infectious keratitis. Methods First we performed in vivo confocal microscopy (HRT III, Rostock Cornea Module) in 118 patients diagnosed for the infectious keratitis (45 viral, 40 bacterial, 23 fungal, 10 amoebal). Inflammatory cytology has been analyzed according to morphology and size of cells forming infiltration. Microscopic scans were then processed with stereological software (MicroBrightField Inc, VT, USA) to track inflammatory cells within scans. Representative reconstructions of inflammatory cells infiltration has been created for each etiology of keratitis based on previous characterization of cells. Results Overall inflammatory cells densities showed no specificity for keratitis etiology, however there was clearly different participation of morphological types of cells depended on keratitis etiology. Leukocyte‐like, round cells represented approximately 4.4% of inflammatory cells in viral, 91.2% in bacterial, 50.4% in fungal and 54.4% in amoebal keratitis. Rest of cells were represented by different forms of dendritic cells, which were possible to track in stereology. Conclusions In vivo analysis of corneal epithelial cytology provides useful information about keratitis etiology. Reconstruction of inflammatory cells infiltration can help to create diagnostic algorithm for infectious keratitis diagnosis.
doi_str_mv 10.1111/j.1755-3768.2015.0501
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1715754129</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3815679721</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1721-b507054b6dce12112c3a5462f5232bb99aede6757d815e41681002c34d113e433</originalsourceid><addsrcrecordid>eNqNkN9KwzAUxoMoOKePIBS8bs1J82fzbgz_wWAXm-BdSNsUUttmJimyOx_BZ_RJbJzs2hDIyXe-7xz4IXQNOIPx3DYZCMbSXPBZRjCwDDMMJ2hyVE-PNXs9RxfeNxhz4JxO0HajO_P9-aWGYDsVdJU4XdreBzeUwdg-sXVi-rpV3di1bh8_pg1O_TZNvLWOzsEnbzrKwfhLdFar1uurv3eKXh7ut8undLV-fF4uVmkJgkBaMCwwowWvSg0EgJS5YpSTmpGcFMV8rnSluWCimgHTFPgMMB5NtALINc3zKbo5zN05-z5oH2RjB9ePKyUIYIJRIPPRxQ6u0lnvna7lzplOub0ELCNA2ciIR0ZUMgKUEeCYuzvkPkyr9_8LycV68xv-Afjidko</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1715754129</pqid></control><display><type>article</type><title>Semi‐automated reconstruction of inflammatory infiltration in infectious keratitis</title><source>Wiley Free Content</source><source>Wiley Online Library All Journals</source><creator>Smedowski, A. ; Tarnawska, D. ; Wylegala, E.</creator><creatorcontrib>Smedowski, A. ; Tarnawska, D. ; Wylegala, E.</creatorcontrib><description>Purpose To perform reconstruction of inflammatory cells infiltration in infectious keratitis. Methods First we performed in vivo confocal microscopy (HRT III, Rostock Cornea Module) in 118 patients diagnosed for the infectious keratitis (45 viral, 40 bacterial, 23 fungal, 10 amoebal). Inflammatory cytology has been analyzed according to morphology and size of cells forming infiltration. Microscopic scans were then processed with stereological software (MicroBrightField Inc, VT, USA) to track inflammatory cells within scans. Representative reconstructions of inflammatory cells infiltration has been created for each etiology of keratitis based on previous characterization of cells. Results Overall inflammatory cells densities showed no specificity for keratitis etiology, however there was clearly different participation of morphological types of cells depended on keratitis etiology. Leukocyte‐like, round cells represented approximately 4.4% of inflammatory cells in viral, 91.2% in bacterial, 50.4% in fungal and 54.4% in amoebal keratitis. Rest of cells were represented by different forms of dendritic cells, which were possible to track in stereology. Conclusions In vivo analysis of corneal epithelial cytology provides useful information about keratitis etiology. Reconstruction of inflammatory cells infiltration can help to create diagnostic algorithm for infectious keratitis diagnosis.</description><identifier>ISSN: 1755-375X</identifier><identifier>EISSN: 1755-3768</identifier><identifier>DOI: 10.1111/j.1755-3768.2015.0501</identifier><language>eng</language><publisher>Malden: Wiley Subscription Services, Inc</publisher><subject>Cellular biology ; Ophthalmology ; Pancreas</subject><ispartof>Acta ophthalmologica (Oxford, England), 2015-10, Vol.93 (S255), p.n/a</ispartof><rights>2015 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley &amp; Sons Ltd</rights><rights>Copyright © 2015 Acta Ophthalmologica Scandinavica Foundation</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1755-3768.2015.0501$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45575,46833</link.rule.ids></links><search><creatorcontrib>Smedowski, A.</creatorcontrib><creatorcontrib>Tarnawska, D.</creatorcontrib><creatorcontrib>Wylegala, E.</creatorcontrib><title>Semi‐automated reconstruction of inflammatory infiltration in infectious keratitis</title><title>Acta ophthalmologica (Oxford, England)</title><description>Purpose To perform reconstruction of inflammatory cells infiltration in infectious keratitis. Methods First we performed in vivo confocal microscopy (HRT III, Rostock Cornea Module) in 118 patients diagnosed for the infectious keratitis (45 viral, 40 bacterial, 23 fungal, 10 amoebal). Inflammatory cytology has been analyzed according to morphology and size of cells forming infiltration. Microscopic scans were then processed with stereological software (MicroBrightField Inc, VT, USA) to track inflammatory cells within scans. Representative reconstructions of inflammatory cells infiltration has been created for each etiology of keratitis based on previous characterization of cells. Results Overall inflammatory cells densities showed no specificity for keratitis etiology, however there was clearly different participation of morphological types of cells depended on keratitis etiology. Leukocyte‐like, round cells represented approximately 4.4% of inflammatory cells in viral, 91.2% in bacterial, 50.4% in fungal and 54.4% in amoebal keratitis. Rest of cells were represented by different forms of dendritic cells, which were possible to track in stereology. Conclusions In vivo analysis of corneal epithelial cytology provides useful information about keratitis etiology. Reconstruction of inflammatory cells infiltration can help to create diagnostic algorithm for infectious keratitis diagnosis.</description><subject>Cellular biology</subject><subject>Ophthalmology</subject><subject>Pancreas</subject><issn>1755-375X</issn><issn>1755-3768</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkN9KwzAUxoMoOKePIBS8bs1J82fzbgz_wWAXm-BdSNsUUttmJimyOx_BZ_RJbJzs2hDIyXe-7xz4IXQNOIPx3DYZCMbSXPBZRjCwDDMMJ2hyVE-PNXs9RxfeNxhz4JxO0HajO_P9-aWGYDsVdJU4XdreBzeUwdg-sXVi-rpV3di1bh8_pg1O_TZNvLWOzsEnbzrKwfhLdFar1uurv3eKXh7ut8undLV-fF4uVmkJgkBaMCwwowWvSg0EgJS5YpSTmpGcFMV8rnSluWCimgHTFPgMMB5NtALINc3zKbo5zN05-z5oH2RjB9ePKyUIYIJRIPPRxQ6u0lnvna7lzplOub0ELCNA2ciIR0ZUMgKUEeCYuzvkPkyr9_8LycV68xv-Afjidko</recordid><startdate>201510</startdate><enddate>201510</enddate><creator>Smedowski, A.</creator><creator>Tarnawska, D.</creator><creator>Wylegala, E.</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>201510</creationdate><title>Semi‐automated reconstruction of inflammatory infiltration in infectious keratitis</title><author>Smedowski, A. ; Tarnawska, D. ; Wylegala, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1721-b507054b6dce12112c3a5462f5232bb99aede6757d815e41681002c34d113e433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cellular biology</topic><topic>Ophthalmology</topic><topic>Pancreas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smedowski, A.</creatorcontrib><creatorcontrib>Tarnawska, D.</creatorcontrib><creatorcontrib>Wylegala, E.</creatorcontrib><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Acta ophthalmologica (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smedowski, A.</au><au>Tarnawska, D.</au><au>Wylegala, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Semi‐automated reconstruction of inflammatory infiltration in infectious keratitis</atitle><jtitle>Acta ophthalmologica (Oxford, England)</jtitle><date>2015-10</date><risdate>2015</risdate><volume>93</volume><issue>S255</issue><epage>n/a</epage><issn>1755-375X</issn><eissn>1755-3768</eissn><abstract>Purpose To perform reconstruction of inflammatory cells infiltration in infectious keratitis. Methods First we performed in vivo confocal microscopy (HRT III, Rostock Cornea Module) in 118 patients diagnosed for the infectious keratitis (45 viral, 40 bacterial, 23 fungal, 10 amoebal). Inflammatory cytology has been analyzed according to morphology and size of cells forming infiltration. Microscopic scans were then processed with stereological software (MicroBrightField Inc, VT, USA) to track inflammatory cells within scans. Representative reconstructions of inflammatory cells infiltration has been created for each etiology of keratitis based on previous characterization of cells. Results Overall inflammatory cells densities showed no specificity for keratitis etiology, however there was clearly different participation of morphological types of cells depended on keratitis etiology. Leukocyte‐like, round cells represented approximately 4.4% of inflammatory cells in viral, 91.2% in bacterial, 50.4% in fungal and 54.4% in amoebal keratitis. Rest of cells were represented by different forms of dendritic cells, which were possible to track in stereology. Conclusions In vivo analysis of corneal epithelial cytology provides useful information about keratitis etiology. Reconstruction of inflammatory cells infiltration can help to create diagnostic algorithm for infectious keratitis diagnosis.</abstract><cop>Malden</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/j.1755-3768.2015.0501</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1755-375X
ispartof Acta ophthalmologica (Oxford, England), 2015-10, Vol.93 (S255), p.n/a
issn 1755-375X
1755-3768
language eng
recordid cdi_proquest_journals_1715754129
source Wiley Free Content; Wiley Online Library All Journals
subjects Cellular biology
Ophthalmology
Pancreas
title Semi‐automated reconstruction of inflammatory infiltration in infectious keratitis
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T02%3A38%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Semi%E2%80%90automated%20reconstruction%20of%20inflammatory%20infiltration%20in%20infectious%20keratitis&rft.jtitle=Acta%20ophthalmologica%20(Oxford,%20England)&rft.au=Smedowski,%20A.&rft.date=2015-10&rft.volume=93&rft.issue=S255&rft.epage=n/a&rft.issn=1755-375X&rft.eissn=1755-3768&rft_id=info:doi/10.1111/j.1755-3768.2015.0501&rft_dat=%3Cproquest_cross%3E3815679721%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1715754129&rft_id=info:pmid/&rfr_iscdi=true