The Hybrid Pyrroloisoindolone-Dehydropyrrolizine Alkaloid (-)-ChlorizidineA Targets Proteins within the Glycolytic Pathway

The Hybrid Pyrroloisoindolone-Dehydropyrrolizine Alkaloid (-)-ChlorizidineA Targets Proteins within the Glycolytic Pathway

The cytotoxic activity of (-)-chlorizidineA, a marine alkaloid containing a unique fusion between a pyrroloisoindolone and dehydropyrrolizine, was explored by using a combination of cellular and molecular methods. Our studies began by applying preliminary SAR evidence gathered from semisynthetic bio...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2015-09, Vol.16 (14), p.2002
Hauptverfasser: Alvarez-Mico, Xavier, Rocha, Danilo D, Guimaraes, Larissa A, Ambrose, Andrew, Chapman, Eli, Costa-Lotufo, Leticia V, LaClair, James J, Fenical, William
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container_issue 14
container_start_page 2002
container_title Chembiochem : a European journal of chemical biology
container_volume 16
creator Alvarez-Mico, Xavier
Rocha, Danilo D
Guimaraes, Larissa A
Ambrose, Andrew
Chapman, Eli
Costa-Lotufo, Leticia V
LaClair, James J
Fenical, William
description The cytotoxic activity of (-)-chlorizidineA, a marine alkaloid containing a unique fusion between a pyrroloisoindolone and dehydropyrrolizine, was explored by using a combination of cellular and molecular methods. Our studies began by applying preliminary SAR evidence gathered from semisynthetic bioactivity evaluations to prepare an active immunoaffinity fluorescent (IAF) probe. This probe was then used to identify two cytosolic proteins, GAPDH and hENO1, as the targets of (-)-chlorizidineA.
doi_str_mv 10.1002/cbic.201500229
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title The Hybrid Pyrroloisoindolone-Dehydropyrrolizine Alkaloid (-)-ChlorizidineA Targets Proteins within the Glycolytic Pathway
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