Comparison of epitheliotrophic factors in autologous serum eyedrops from sera of chronic renal failure patients vs. normal controls
Purpose To investigate the composition and biological activity of serum epitheliotrophic factors from chronic renal failure (CRF) patients vs. healthy controls. Methods Twenty, 50 and 100 % autologous serum eyedrops (ASEs) were prepared from 16 CRF patients and 16 normal subjects. Serum epithelial g...
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| creator | Kang, Na Hee Lee, Shina Jun, Roo Min |
| description | Purpose
To investigate the composition and biological activity of serum epitheliotrophic factors from chronic renal failure (CRF) patients vs. healthy controls.
Methods
Twenty, 50 and 100 % autologous serum eyedrops (ASEs) were prepared from 16 CRF patients and 16 normal subjects. Serum epithelial growth factor (EGF), platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-β
1
(TGF-β
1
) and fibronectin levels were quantified using enzyme-linked immunosorbent assay kits. A 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide (MTT) assay was used to compare the proliferative effects of serum from CRF patients and healthy donors in a human corneal epithelial cell (HCEC) culture model. Migration assays were conducted via manual scraping of HCECs for migratory potential of ASEs. Morphologic changes were studied with transmission electron microscopy (TEM).
Results
EGF, PDGF-AB and TGF-β
1
levels in ASEs from healthy donors were significantly higher than in serum of CRF patients. Cellular proliferation was similar in the CRF patient and normal control groups. ASEs from the normal group had a significantly higher effect on cell migration. ASEs in both groups facilitated better proliferation and migration than the negative control. Furthermore, we observed an enhancement after incubation with diluted serum vs. undiluted serum. In TEM analysis, HCECs incubated with CRF patients’ 50 % ASEs showed some loss of microvilli without alterations of cytoplasmic organelles.
Conclusions
Epitheliotrophic factors concentrations and biologic activities from CRF patient sera differed from healthy controls. ASEs in CRF patients are also helpful in the corneal healing process, especially when applied at a 50 % concentration. |
| doi_str_mv | 10.1007/s00417-015-3056-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1712262021</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3806538591</sourcerecordid><originalsourceid>FETCH-LOGICAL-c409t-cf185022ed89a92e1724710880a1c617e51eb173eee697358018922dad3e55d93</originalsourceid><addsrcrecordid>eNp1kE1L7TAQhoNc0ePHD3AjgbuuziQnTbuUg18guFFwF2I79VTapjfTCq794-ZwvOLGVSDzvO8MjxAnCGcIYM8ZYIk2AzSZBpNnZkcscKlNZkE9_RELsAqzQqunfXHA_AoJ1wb3xL7KQeWlNgvxsQr96GPLYZChkTS205q6NkwxjOu2ko2vphBZtoP08xS68BJmlkxx7iW9U50wlk0M_ebPbyqqdQxDSkYafJfybTdHkqOfWhomlm98JocQ-zSrwpDWdHwkdhvfMR1_vYfi8eryYXWT3d1f364u7rJqCeWUVQ0WBpSiuih9qQitWlqEogCPVY6WDNIzWk1EeWm1KQCLUqna15qMqUt9KP5ue8cY_s3Ek3sNc0xXskOLSuUKFCYKt1QVA3Okxo2x7X18dwhuo91ttbuk3W20O5Myp1_N83NP9Xfiv-cEqC3AaTS8UPyx-tfWT7yfj0g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1712262021</pqid></control><display><type>article</type><title>Comparison of epitheliotrophic factors in autologous serum eyedrops from sera of chronic renal failure patients vs. normal controls</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Kang, Na Hee ; Lee, Shina ; Jun, Roo Min</creator><creatorcontrib>Kang, Na Hee ; Lee, Shina ; Jun, Roo Min</creatorcontrib><description>Purpose
To investigate the composition and biological activity of serum epitheliotrophic factors from chronic renal failure (CRF) patients vs. healthy controls.
Methods
Twenty, 50 and 100 % autologous serum eyedrops (ASEs) were prepared from 16 CRF patients and 16 normal subjects. Serum epithelial growth factor (EGF), platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-β
1
(TGF-β
1
) and fibronectin levels were quantified using enzyme-linked immunosorbent assay kits. A 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide (MTT) assay was used to compare the proliferative effects of serum from CRF patients and healthy donors in a human corneal epithelial cell (HCEC) culture model. Migration assays were conducted via manual scraping of HCECs for migratory potential of ASEs. Morphologic changes were studied with transmission electron microscopy (TEM).
Results
EGF, PDGF-AB and TGF-β
1
levels in ASEs from healthy donors were significantly higher than in serum of CRF patients. Cellular proliferation was similar in the CRF patient and normal control groups. ASEs from the normal group had a significantly higher effect on cell migration. ASEs in both groups facilitated better proliferation and migration than the negative control. Furthermore, we observed an enhancement after incubation with diluted serum vs. undiluted serum. In TEM analysis, HCECs incubated with CRF patients’ 50 % ASEs showed some loss of microvilli without alterations of cytoplasmic organelles.
Conclusions
Epitheliotrophic factors concentrations and biologic activities from CRF patient sera differed from healthy controls. ASEs in CRF patients are also helpful in the corneal healing process, especially when applied at a 50 % concentration.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s00417-015-3056-5</identifier><identifier>PMID: 26026935</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Aged ; Basic Science ; Cell Line ; Cell Movement - physiology ; Cell Proliferation - physiology ; Cells, Cultured ; Enzyme-Linked Immunosorbent Assay ; Epithelium, Corneal - cytology ; Female ; Fibronectins - analysis ; Healthy Volunteers ; Humans ; Kidney Failure, Chronic - blood ; Male ; Medicine ; Medicine & Public Health ; Microscopy, Electron, Transmission ; Middle Aged ; Ophthalmic Solutions ; Ophthalmology ; Platelet-Derived Growth Factor - analysis ; Receptor, Epidermal Growth Factor - analysis ; Serum - chemistry ; Serum - physiology ; Transforming Growth Factor beta1 - analysis</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 2015-10, Vol.253 (10), p.1705-1712</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-cf185022ed89a92e1724710880a1c617e51eb173eee697358018922dad3e55d93</citedby><cites>FETCH-LOGICAL-c409t-cf185022ed89a92e1724710880a1c617e51eb173eee697358018922dad3e55d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00417-015-3056-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00417-015-3056-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26026935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kang, Na Hee</creatorcontrib><creatorcontrib>Lee, Shina</creatorcontrib><creatorcontrib>Jun, Roo Min</creatorcontrib><title>Comparison of epitheliotrophic factors in autologous serum eyedrops from sera of chronic renal failure patients vs. normal controls</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Purpose
To investigate the composition and biological activity of serum epitheliotrophic factors from chronic renal failure (CRF) patients vs. healthy controls.
Methods
Twenty, 50 and 100 % autologous serum eyedrops (ASEs) were prepared from 16 CRF patients and 16 normal subjects. Serum epithelial growth factor (EGF), platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-β
1
(TGF-β
1
) and fibronectin levels were quantified using enzyme-linked immunosorbent assay kits. A 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide (MTT) assay was used to compare the proliferative effects of serum from CRF patients and healthy donors in a human corneal epithelial cell (HCEC) culture model. Migration assays were conducted via manual scraping of HCECs for migratory potential of ASEs. Morphologic changes were studied with transmission electron microscopy (TEM).
Results
EGF, PDGF-AB and TGF-β
1
levels in ASEs from healthy donors were significantly higher than in serum of CRF patients. Cellular proliferation was similar in the CRF patient and normal control groups. ASEs from the normal group had a significantly higher effect on cell migration. ASEs in both groups facilitated better proliferation and migration than the negative control. Furthermore, we observed an enhancement after incubation with diluted serum vs. undiluted serum. In TEM analysis, HCECs incubated with CRF patients’ 50 % ASEs showed some loss of microvilli without alterations of cytoplasmic organelles.
Conclusions
Epitheliotrophic factors concentrations and biologic activities from CRF patient sera differed from healthy controls. ASEs in CRF patients are also helpful in the corneal healing process, especially when applied at a 50 % concentration.</description><subject>Adult</subject><subject>Aged</subject><subject>Basic Science</subject><subject>Cell Line</subject><subject>Cell Movement - physiology</subject><subject>Cell Proliferation - physiology</subject><subject>Cells, Cultured</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epithelium, Corneal - cytology</subject><subject>Female</subject><subject>Fibronectins - analysis</subject><subject>Healthy Volunteers</subject><subject>Humans</subject><subject>Kidney Failure, Chronic - blood</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microscopy, Electron, Transmission</subject><subject>Middle Aged</subject><subject>Ophthalmic Solutions</subject><subject>Ophthalmology</subject><subject>Platelet-Derived Growth Factor - analysis</subject><subject>Receptor, Epidermal Growth Factor - analysis</subject><subject>Serum - chemistry</subject><subject>Serum - physiology</subject><subject>Transforming Growth Factor beta1 - analysis</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kE1L7TAQhoNc0ePHD3AjgbuuziQnTbuUg18guFFwF2I79VTapjfTCq794-ZwvOLGVSDzvO8MjxAnCGcIYM8ZYIk2AzSZBpNnZkcscKlNZkE9_RELsAqzQqunfXHA_AoJ1wb3xL7KQeWlNgvxsQr96GPLYZChkTS205q6NkwxjOu2ko2vphBZtoP08xS68BJmlkxx7iW9U50wlk0M_ebPbyqqdQxDSkYafJfybTdHkqOfWhomlm98JocQ-zSrwpDWdHwkdhvfMR1_vYfi8eryYXWT3d1f364u7rJqCeWUVQ0WBpSiuih9qQitWlqEogCPVY6WDNIzWk1EeWm1KQCLUqna15qMqUt9KP5ue8cY_s3Ek3sNc0xXskOLSuUKFCYKt1QVA3Okxo2x7X18dwhuo91ttbuk3W20O5Myp1_N83NP9Xfiv-cEqC3AaTS8UPyx-tfWT7yfj0g</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Kang, Na Hee</creator><creator>Lee, Shina</creator><creator>Jun, Roo Min</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20151001</creationdate><title>Comparison of epitheliotrophic factors in autologous serum eyedrops from sera of chronic renal failure patients vs. normal controls</title><author>Kang, Na Hee ; Lee, Shina ; Jun, Roo Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-cf185022ed89a92e1724710880a1c617e51eb173eee697358018922dad3e55d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Basic Science</topic><topic>Cell Line</topic><topic>Cell Movement - physiology</topic><topic>Cell Proliferation - physiology</topic><topic>Cells, Cultured</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epithelium, Corneal - cytology</topic><topic>Female</topic><topic>Fibronectins - analysis</topic><topic>Healthy Volunteers</topic><topic>Humans</topic><topic>Kidney Failure, Chronic - blood</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microscopy, Electron, Transmission</topic><topic>Middle Aged</topic><topic>Ophthalmic Solutions</topic><topic>Ophthalmology</topic><topic>Platelet-Derived Growth Factor - analysis</topic><topic>Receptor, Epidermal Growth Factor - analysis</topic><topic>Serum - chemistry</topic><topic>Serum - physiology</topic><topic>Transforming Growth Factor beta1 - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kang, Na Hee</creatorcontrib><creatorcontrib>Lee, Shina</creatorcontrib><creatorcontrib>Jun, Roo Min</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kang, Na Hee</au><au>Lee, Shina</au><au>Jun, Roo Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of epitheliotrophic factors in autologous serum eyedrops from sera of chronic renal failure patients vs. normal controls</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><stitle>Graefes Arch Clin Exp Ophthalmol</stitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>253</volume><issue>10</issue><spage>1705</spage><epage>1712</epage><pages>1705-1712</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><abstract>Purpose
To investigate the composition and biological activity of serum epitheliotrophic factors from chronic renal failure (CRF) patients vs. healthy controls.
Methods
Twenty, 50 and 100 % autologous serum eyedrops (ASEs) were prepared from 16 CRF patients and 16 normal subjects. Serum epithelial growth factor (EGF), platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-β
1
(TGF-β
1
) and fibronectin levels were quantified using enzyme-linked immunosorbent assay kits. A 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide (MTT) assay was used to compare the proliferative effects of serum from CRF patients and healthy donors in a human corneal epithelial cell (HCEC) culture model. Migration assays were conducted via manual scraping of HCECs for migratory potential of ASEs. Morphologic changes were studied with transmission electron microscopy (TEM).
Results
EGF, PDGF-AB and TGF-β
1
levels in ASEs from healthy donors were significantly higher than in serum of CRF patients. Cellular proliferation was similar in the CRF patient and normal control groups. ASEs from the normal group had a significantly higher effect on cell migration. ASEs in both groups facilitated better proliferation and migration than the negative control. Furthermore, we observed an enhancement after incubation with diluted serum vs. undiluted serum. In TEM analysis, HCECs incubated with CRF patients’ 50 % ASEs showed some loss of microvilli without alterations of cytoplasmic organelles.
Conclusions
Epitheliotrophic factors concentrations and biologic activities from CRF patient sera differed from healthy controls. ASEs in CRF patients are also helpful in the corneal healing process, especially when applied at a 50 % concentration.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26026935</pmid><doi>10.1007/s00417-015-3056-5</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-832X |
| ispartof | Graefe's archive for clinical and experimental ophthalmology, 2015-10, Vol.253 (10), p.1705-1712 |
| issn | 0721-832X 1435-702X |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adult Aged Basic Science Cell Line Cell Movement - physiology Cell Proliferation - physiology Cells, Cultured Enzyme-Linked Immunosorbent Assay Epithelium, Corneal - cytology Female Fibronectins - analysis Healthy Volunteers Humans Kidney Failure, Chronic - blood Male Medicine Medicine & Public Health Microscopy, Electron, Transmission Middle Aged Ophthalmic Solutions Ophthalmology Platelet-Derived Growth Factor - analysis Receptor, Epidermal Growth Factor - analysis Serum - chemistry Serum - physiology Transforming Growth Factor beta1 - analysis |
| title | Comparison of epitheliotrophic factors in autologous serum eyedrops from sera of chronic renal failure patients vs. normal controls |
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