MCT1 and MCT4 kinetic of mRNA expression in different tissues after aerobic exercise at maximal lactate steady state workload
We evaluate the mRNA expression of monocarboxylate transporters 1 and 4 (MCT1 and MCT4) in skeletal muscle (soleus, red and white gastrocnemius), heart and liver tissues in mice submitted to a single bout of swimming exercise at the maximal lactate steady state workload (MLSSw). After 72 h of MLSS t...
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| Veröffentlicht in: | Physiological research 2015-01, Vol.64 (4), p.513-522 |
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| creator | de Araujo, G G Gobatto, C A de Barros Manchado-Gobatto, F Teixeira, L Fm Dos Reis, I Gm Caperuto, L C Papoti, M Bordin, S Cavaglieri, C R Verlengia, R |
| description | We evaluate the mRNA expression of monocarboxylate transporters 1 and 4 (MCT1 and MCT4) in skeletal muscle (soleus, red and white gastrocnemius), heart and liver tissues in mice submitted to a single bout of swimming exercise at the maximal lactate steady state workload (MLSSw). After 72 h of MLSS test, the animals were submitted to a swimming exercise session for 25 min at individual MLSSw. Tissues and muscle samples were obtained at rest (control, n=5), immediately (n=5), 5 h (n=5) and 10 h (n=5) after exercise for determination of the MCT1 and MCT4 mRNA expression (RT-PCR). The MCT1 mRNA expression in liver increased after 10 h in relation to the control, immediate and 5 h groups, but the MCT4 remained unchanged. The MCT1 mRNA expression in heart increased by 31 % after 10 h when compared to immediate, but no differences were observed in relation to the control group. No significant differences were observed for red gastrocnemius in MCT1 and MCT4 mRNA expression. However, white gastrocnemius increased MCT1 mRNA expression immediately when compared to rest, 5 and 10 h test groups. In soleus muscle, the MCT1 mRNA expression increased immediately, 5 and 10 h after exercise when compared to the control. In relation to MCT4 mRNA expression, the soleus increased immediately and 10 h after acute exercise when compared to the control group. The soleus, liver and heart were the main tissues that showed improved the MCT1 mRNA expression, indicating its important role in controlling MLSS concentration in mice. |
| doi_str_mv | 10.33549/physiolres.932695 |
| format | Article |
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After 72 h of MLSS test, the animals were submitted to a swimming exercise session for 25 min at individual MLSSw. Tissues and muscle samples were obtained at rest (control, n=5), immediately (n=5), 5 h (n=5) and 10 h (n=5) after exercise for determination of the MCT1 and MCT4 mRNA expression (RT-PCR). The MCT1 mRNA expression in liver increased after 10 h in relation to the control, immediate and 5 h groups, but the MCT4 remained unchanged. The MCT1 mRNA expression in heart increased by 31 % after 10 h when compared to immediate, but no differences were observed in relation to the control group. No significant differences were observed for red gastrocnemius in MCT1 and MCT4 mRNA expression. However, white gastrocnemius increased MCT1 mRNA expression immediately when compared to rest, 5 and 10 h test groups. In soleus muscle, the MCT1 mRNA expression increased immediately, 5 and 10 h after exercise when compared to the control. In relation to MCT4 mRNA expression, the soleus increased immediately and 10 h after acute exercise when compared to the control group. The soleus, liver and heart were the main tissues that showed improved the MCT1 mRNA expression, indicating its important role in controlling MLSS concentration in mice.</description><identifier>ISSN: 0862-8408</identifier><identifier>EISSN: 1802-9973</identifier><identifier>DOI: 10.33549/physiolres.932695</identifier><identifier>PMID: 25470525</identifier><language>eng</language><publisher>Czech Republic: Institute of Physiology</publisher><subject>Anaerobic Threshold - physiology ; Animals ; Equilibrium ; Exercise ; Experiments ; Gene expression ; Heart ; Laboratories ; Liver - metabolism ; Male ; Mice ; Mice, Inbred C57BL ; MicroRNAs - metabolism ; Monocarboxylic Acid Transporters - genetics ; Monocarboxylic Acid Transporters - metabolism ; Muscle, Skeletal - physiology ; Myocardium - metabolism ; Organ Specificity ; Physical Conditioning, Animal - methods ; Physical Exertion - physiology ; Physical fitness ; Proteins ; Rodents ; Swimming - physiology ; Symporters - genetics ; Symporters - metabolism ; Tissue Distribution</subject><ispartof>Physiological research, 2015-01, Vol.64 (4), p.513-522</ispartof><rights>Copyright Institute of Physiology 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-fe7ed94a6e0002e683e248ddce3b170053b6127bd2a3c392dec90f9eaca62b953</citedby><cites>FETCH-LOGICAL-c408t-fe7ed94a6e0002e683e248ddce3b170053b6127bd2a3c392dec90f9eaca62b953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25470525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Araujo, G G</creatorcontrib><creatorcontrib>Gobatto, C A</creatorcontrib><creatorcontrib>de Barros Manchado-Gobatto, F</creatorcontrib><creatorcontrib>Teixeira, L Fm</creatorcontrib><creatorcontrib>Dos Reis, I Gm</creatorcontrib><creatorcontrib>Caperuto, L C</creatorcontrib><creatorcontrib>Papoti, M</creatorcontrib><creatorcontrib>Bordin, S</creatorcontrib><creatorcontrib>Cavaglieri, C R</creatorcontrib><creatorcontrib>Verlengia, R</creatorcontrib><title>MCT1 and MCT4 kinetic of mRNA expression in different tissues after aerobic exercise at maximal lactate steady state workload</title><title>Physiological research</title><addtitle>Physiol Res</addtitle><description>We evaluate the mRNA expression of monocarboxylate transporters 1 and 4 (MCT1 and MCT4) in skeletal muscle (soleus, red and white gastrocnemius), heart and liver tissues in mice submitted to a single bout of swimming exercise at the maximal lactate steady state workload (MLSSw). After 72 h of MLSS test, the animals were submitted to a swimming exercise session for 25 min at individual MLSSw. Tissues and muscle samples were obtained at rest (control, n=5), immediately (n=5), 5 h (n=5) and 10 h (n=5) after exercise for determination of the MCT1 and MCT4 mRNA expression (RT-PCR). The MCT1 mRNA expression in liver increased after 10 h in relation to the control, immediate and 5 h groups, but the MCT4 remained unchanged. The MCT1 mRNA expression in heart increased by 31 % after 10 h when compared to immediate, but no differences were observed in relation to the control group. No significant differences were observed for red gastrocnemius in MCT1 and MCT4 mRNA expression. However, white gastrocnemius increased MCT1 mRNA expression immediately when compared to rest, 5 and 10 h test groups. In soleus muscle, the MCT1 mRNA expression increased immediately, 5 and 10 h after exercise when compared to the control. In relation to MCT4 mRNA expression, the soleus increased immediately and 10 h after acute exercise when compared to the control group. The soleus, liver and heart were the main tissues that showed improved the MCT1 mRNA expression, indicating its important role in controlling MLSS concentration in mice.</description><subject>Anaerobic Threshold - physiology</subject><subject>Animals</subject><subject>Equilibrium</subject><subject>Exercise</subject><subject>Experiments</subject><subject>Gene expression</subject><subject>Heart</subject><subject>Laboratories</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>MicroRNAs - metabolism</subject><subject>Monocarboxylic Acid Transporters - genetics</subject><subject>Monocarboxylic Acid Transporters - metabolism</subject><subject>Muscle, Skeletal - physiology</subject><subject>Myocardium - metabolism</subject><subject>Organ Specificity</subject><subject>Physical Conditioning, Animal - methods</subject><subject>Physical Exertion - physiology</subject><subject>Physical fitness</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Swimming - physiology</subject><subject>Symporters - genetics</subject><subject>Symporters - metabolism</subject><subject>Tissue 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and MCT4 kinetic of mRNA expression in different tissues after aerobic exercise at maximal lactate steady state workload</title><author>de Araujo, G G ; Gobatto, C A ; de Barros Manchado-Gobatto, F ; Teixeira, L Fm ; Dos Reis, I Gm ; Caperuto, L C ; Papoti, M ; Bordin, S ; Cavaglieri, C R ; Verlengia, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-fe7ed94a6e0002e683e248ddce3b170053b6127bd2a3c392dec90f9eaca62b953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anaerobic Threshold - physiology</topic><topic>Animals</topic><topic>Equilibrium</topic><topic>Exercise</topic><topic>Experiments</topic><topic>Gene expression</topic><topic>Heart</topic><topic>Laboratories</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>MicroRNAs - metabolism</topic><topic>Monocarboxylic Acid Transporters - genetics</topic><topic>Monocarboxylic Acid Transporters - metabolism</topic><topic>Muscle, Skeletal - physiology</topic><topic>Myocardium - metabolism</topic><topic>Organ Specificity</topic><topic>Physical Conditioning, Animal - methods</topic><topic>Physical Exertion - physiology</topic><topic>Physical fitness</topic><topic>Proteins</topic><topic>Rodents</topic><topic>Swimming - physiology</topic><topic>Symporters - genetics</topic><topic>Symporters - metabolism</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Araujo, G G</creatorcontrib><creatorcontrib>Gobatto, C A</creatorcontrib><creatorcontrib>de Barros Manchado-Gobatto, F</creatorcontrib><creatorcontrib>Teixeira, L Fm</creatorcontrib><creatorcontrib>Dos Reis, I Gm</creatorcontrib><creatorcontrib>Caperuto, L C</creatorcontrib><creatorcontrib>Papoti, M</creatorcontrib><creatorcontrib>Bordin, S</creatorcontrib><creatorcontrib>Cavaglieri, C 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C</au><au>Papoti, M</au><au>Bordin, S</au><au>Cavaglieri, C R</au><au>Verlengia, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MCT1 and MCT4 kinetic of mRNA expression in different tissues after aerobic exercise at maximal lactate steady state workload</atitle><jtitle>Physiological research</jtitle><addtitle>Physiol Res</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>64</volume><issue>4</issue><spage>513</spage><epage>522</epage><pages>513-522</pages><issn>0862-8408</issn><eissn>1802-9973</eissn><abstract>We evaluate the mRNA expression of monocarboxylate transporters 1 and 4 (MCT1 and MCT4) in skeletal muscle (soleus, red and white gastrocnemius), heart and liver tissues in mice submitted to a single bout of swimming exercise at the maximal lactate steady state workload (MLSSw). After 72 h of MLSS test, the animals were submitted to a swimming exercise session for 25 min at individual MLSSw. Tissues and muscle samples were obtained at rest (control, n=5), immediately (n=5), 5 h (n=5) and 10 h (n=5) after exercise for determination of the MCT1 and MCT4 mRNA expression (RT-PCR). The MCT1 mRNA expression in liver increased after 10 h in relation to the control, immediate and 5 h groups, but the MCT4 remained unchanged. The MCT1 mRNA expression in heart increased by 31 % after 10 h when compared to immediate, but no differences were observed in relation to the control group. No significant differences were observed for red gastrocnemius in MCT1 and MCT4 mRNA expression. However, white gastrocnemius increased MCT1 mRNA expression immediately when compared to rest, 5 and 10 h test groups. In soleus muscle, the MCT1 mRNA expression increased immediately, 5 and 10 h after exercise when compared to the control. In relation to MCT4 mRNA expression, the soleus increased immediately and 10 h after acute exercise when compared to the control group. The soleus, liver and heart were the main tissues that showed improved the MCT1 mRNA expression, indicating its important role in controlling MLSS concentration in mice.</abstract><cop>Czech Republic</cop><pub>Institute of Physiology</pub><pmid>25470525</pmid><doi>10.33549/physiolres.932695</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anaerobic Threshold - physiology Animals Equilibrium Exercise Experiments Gene expression Heart Laboratories Liver - metabolism Male Mice Mice, Inbred C57BL MicroRNAs - metabolism Monocarboxylic Acid Transporters - genetics Monocarboxylic Acid Transporters - metabolism Muscle, Skeletal - physiology Myocardium - metabolism Organ Specificity Physical Conditioning, Animal - methods Physical Exertion - physiology Physical fitness Proteins Rodents Swimming - physiology Symporters - genetics Symporters - metabolism Tissue Distribution |
| title | MCT1 and MCT4 kinetic of mRNA expression in different tissues after aerobic exercise at maximal lactate steady state workload |
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