Spray-dried extract of Phyllanthus niruriL. reduces mucosal damage in rats with intestinal inflammation

Spray-dried extract of Phyllanthus niruriL. reduces mucosal damage in rats with intestinal inflammation

Phyllanthus niruriL. belongs to the Euphorbiaceae, and is known by the common name of 'stonebreaker' in Brazil. Some species within the Phyllanthus genus are widely used in traditional medicine to counteract different types of anti-inflammatory diseases. Objectives In this study, the preve...

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Veröffentlicht in:Journal of pharmacy and pharmacology 2015-08, Vol.67 (8), p.1107
Hauptverfasser: Melo, Maryanne Nunes de, Soares, Luiz Alberto Lira, Porto, Cynthia Raquel da Costa, Araujo, Aurigena Antunes de, Almeida, Maria das Graças, Souza, Tatiane Pereira de, Petrovick, Pedro Ros, Araujo, Raimundo Fernandes de, Guerra, Gerlane Coelho Bernardo
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Inflammatory bowel disease
Rodents
Tumor necrosis factor-TNF
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container_issue 8
container_start_page 1107
container_title Journal of pharmacy and pharmacology
container_volume 67
creator Melo, Maryanne Nunes de
Soares, Luiz Alberto Lira
Porto, Cynthia Raquel da Costa
Araujo, Aurigena Antunes de
Almeida, Maria das Graças
Souza, Tatiane Pereira de
Petrovick, Pedro Ros
Araujo, Raimundo Fernandes de
Guerra, Gerlane Coelho Bernardo
description Phyllanthus niruriL. belongs to the Euphorbiaceae, and is known by the common name of 'stonebreaker' in Brazil. Some species within the Phyllanthus genus are widely used in traditional medicine to counteract different types of anti-inflammatory diseases. Objectives In this study, the preventive intestinal anti-inflammatory activity of spray-dried extract of P.niruri (SDEPn) was tested in the model of acetic acid (10%)-induced ulcerative colitis in the rat. Methods Colitis animals were given orally at doses 25, 100 and 200mg/kg. Colons tissue was analysed by macroscopic score, by histopathology score, by the immunohistochemical examination of tumour necrosis factor alpha, p53 and interferon gamma; by spectroscopic ultraviolet-visible spectrophotometry (UV/VIS) analysis of the levels of myeloperoxidase, malonaldehyde and total glutathione. Key Findings/Result Pretreatment of the extract to colitic rats significantly attenuated colonic macroscopic damage induced by acetic acid (P
doi_str_mv 10.1111/jphp.12408
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Some species within the Phyllanthus genus are widely used in traditional medicine to counteract different types of anti-inflammatory diseases. Objectives In this study, the preventive intestinal anti-inflammatory activity of spray-dried extract of P.niruri (SDEPn) was tested in the model of acetic acid (10%)-induced ulcerative colitis in the rat. Methods Colitis animals were given orally at doses 25, 100 and 200mg/kg. Colons tissue was analysed by macroscopic score, by histopathology score, by the immunohistochemical examination of tumour necrosis factor alpha, p53 and interferon gamma; by spectroscopic ultraviolet-visible spectrophotometry (UV/VIS) analysis of the levels of myeloperoxidase, malonaldehyde and total glutathione. Key Findings/Result Pretreatment of the extract to colitic rats significantly attenuated colonic macroscopic damage induced by acetic acid (P&lt;0.01). Spray-dried extract of P.niruri prevented glutathione depletion (P&lt;0.001) and malondialdehyde levels (P&lt;0.05) declined. Spray-dried extract of P.niruri significantly reduced microscopic damage to tissues, such as leukocyte infiltration accompanied by a significant reduction in myeloperoxidase activity (P&lt;0.5). Immunohistochemistry revealed a decline in the TNF-[alpha], IFN-[gamma] and p53 protein (P&lt;0.05). Conclusion Spray-dried extract of P.niruri has a beneficial effect in the acute phase of acetic acid-induced colitis in the rat, which is probably related to its antioxidant properties.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1111/jphp.12408</identifier><identifier>CODEN: JPPMAB</identifier><language>eng</language><publisher>Bognor Regis: Wiley Subscription Services, Inc</publisher><subject>Inflammatory bowel disease ; Rodents ; Tumor necrosis factor-TNF</subject><ispartof>Journal of pharmacy and pharmacology, 2015-08, Vol.67 (8), p.1107</ispartof><rights>Copyright © 2015 Royal Pharmaceutical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Melo, Maryanne Nunes de</creatorcontrib><creatorcontrib>Soares, Luiz Alberto Lira</creatorcontrib><creatorcontrib>Porto, Cynthia Raquel da Costa</creatorcontrib><creatorcontrib>Araujo, Aurigena Antunes de</creatorcontrib><creatorcontrib>Almeida, Maria das Graças</creatorcontrib><creatorcontrib>Souza, Tatiane Pereira de</creatorcontrib><creatorcontrib>Petrovick, Pedro Ros</creatorcontrib><creatorcontrib>Araujo, Raimundo Fernandes de</creatorcontrib><creatorcontrib>Guerra, Gerlane Coelho Bernardo</creatorcontrib><title>Spray-dried extract of Phyllanthus niruriL. reduces mucosal damage in rats with intestinal inflammation</title><title>Journal of pharmacy and pharmacology</title><description>Phyllanthus niruriL. belongs to the Euphorbiaceae, and is known by the common name of 'stonebreaker' in Brazil. Some species within the Phyllanthus genus are widely used in traditional medicine to counteract different types of anti-inflammatory diseases. Objectives In this study, the preventive intestinal anti-inflammatory activity of spray-dried extract of P.niruri (SDEPn) was tested in the model of acetic acid (10%)-induced ulcerative colitis in the rat. Methods Colitis animals were given orally at doses 25, 100 and 200mg/kg. Colons tissue was analysed by macroscopic score, by histopathology score, by the immunohistochemical examination of tumour necrosis factor alpha, p53 and interferon gamma; by spectroscopic ultraviolet-visible spectrophotometry (UV/VIS) analysis of the levels of myeloperoxidase, malonaldehyde and total glutathione. Key Findings/Result Pretreatment of the extract to colitic rats significantly attenuated colonic macroscopic damage induced by acetic acid (P&lt;0.01). Spray-dried extract of P.niruri prevented glutathione depletion (P&lt;0.001) and malondialdehyde levels (P&lt;0.05) declined. Spray-dried extract of P.niruri significantly reduced microscopic damage to tissues, such as leukocyte infiltration accompanied by a significant reduction in myeloperoxidase activity (P&lt;0.5). Immunohistochemistry revealed a decline in the TNF-[alpha], IFN-[gamma] and p53 protein (P&lt;0.05). 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Some species within the Phyllanthus genus are widely used in traditional medicine to counteract different types of anti-inflammatory diseases. Objectives In this study, the preventive intestinal anti-inflammatory activity of spray-dried extract of P.niruri (SDEPn) was tested in the model of acetic acid (10%)-induced ulcerative colitis in the rat. Methods Colitis animals were given orally at doses 25, 100 and 200mg/kg. Colons tissue was analysed by macroscopic score, by histopathology score, by the immunohistochemical examination of tumour necrosis factor alpha, p53 and interferon gamma; by spectroscopic ultraviolet-visible spectrophotometry (UV/VIS) analysis of the levels of myeloperoxidase, malonaldehyde and total glutathione. Key Findings/Result Pretreatment of the extract to colitic rats significantly attenuated colonic macroscopic damage induced by acetic acid (P&lt;0.01). Spray-dried extract of P.niruri prevented glutathione depletion (P&lt;0.001) and malondialdehyde levels (P&lt;0.05) declined. Spray-dried extract of P.niruri significantly reduced microscopic damage to tissues, such as leukocyte infiltration accompanied by a significant reduction in myeloperoxidase activity (P&lt;0.5). Immunohistochemistry revealed a decline in the TNF-[alpha], IFN-[gamma] and p53 protein (P&lt;0.05). Conclusion Spray-dried extract of P.niruri has a beneficial effect in the acute phase of acetic acid-induced colitis in the rat, which is probably related to its antioxidant properties.</abstract><cop>Bognor Regis</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/jphp.12408</doi></addata></record>
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source Oxford University Press Journals All Titles (1996-Current); Wiley Online Library Journals Frontfile Complete
subjects Inflammatory bowel disease
Rodents
Tumor necrosis factor-TNF
title Spray-dried extract of Phyllanthus niruriL. reduces mucosal damage in rats with intestinal inflammation
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