Quantification of Mirafiori lettuce big‐vein virus and its vector, Olpidium virulentus, from soil using real‐time PCR

Big vein disease of lettuce (Lactuca sativa) is an economically important disease transmitted through soil by Olpidium virulentus, and has occurred in most production areas worldwide. The disease is assumed to be caused by Mirafiori lettuce big‐vein virus (MiLBVV). To understand the dynamics of the...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Plant pathology 2015-08, Vol.64 (4), p.825-830
Hauptverfasser: Momonoi, K, Mori, M, Matsuura, K, Moriwaki, J, Morikawa, T
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 830
container_issue 4
container_start_page 825
container_title Plant pathology
container_volume 64
creator Momonoi, K
Mori, M
Matsuura, K
Moriwaki, J
Morikawa, T
description Big vein disease of lettuce (Lactuca sativa) is an economically important disease transmitted through soil by Olpidium virulentus, and has occurred in most production areas worldwide. The disease is assumed to be caused by Mirafiori lettuce big‐vein virus (MiLBVV). To understand the dynamics of the virus and its vector, MiLBVV and O. virulentus were directly detected in soil. DNA and RNA were extracted from 5 g soil using a bead beating method, followed by purification using adsorption to a column. Detection and quantification were performed using real‐time PCR and a TaqMan probe that was prepared based on the CP region of MiLBVV and the rDNA‐ITS region of O. virulentus, respectively. Furthermore, using a visual assessment of the incidence rate of big vein disease on lettuce in agricultural fields, the Cₜ values of MiLBVV and O. virulentus from soil were also determined using real‐time PCR. The results showed that MiLBVV concentrations in the soil were high in the field, as also determined by a visual assessment of the incidence rate of big vein disease on lettuce. However, the amount of O. virulentus in soil was not directly correlated with the incidence of MiLBVV. From these results, it is suggested that the risk of lettuce crops developing big vein disease can be estimated using an index of the amount of MiLBVV in the soil.
doi_str_mv 10.1111/ppa.12333
format Article
fullrecord <record><control><sourceid>proquest_wiley</sourceid><recordid>TN_cdi_proquest_journals_1693598414</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3734842631</sourcerecordid><originalsourceid>FETCH-LOGICAL-f4183-631056859144fe6c68fd2ce91bfc2990b72e3f4c315095af8a294c3aea34d0d43</originalsourceid><addsrcrecordid>eNotkMtOwzAQRS0EEqWw4AuwxJa0fiSpvawqXlJRy6Nry03saqo0DrZT1B2fwDfyJaQts5kZzb13pIPQNSUD2tWwafSAMs75CepRnmcJJ5k8RT1COEuIyNk5ughhTQjNpBQ9tHttdR3BQqEjuBo7i1_AawvOA65MjG1h8BJWv98_WwM13oJvA9Z1iSEGvDVFdP4Oz6oGSmg3h3Nl6tiGO2y92-DgoMJtgHqFvdFVFxNhY_B88naJzqyugrn67320eLj_mDwl09nj82Q8TWxKBU9yTkmWi0zSNLUmL3JhS1YYSZe2YFKS5YgZbtOC04zITFuhmew2bTRPS1KmvI9uj7mNd5-tCVGtXevr7qWiueSZFCndq4ZH1RdUZqcaDxvtd4oStceqOqzqgFXN5-PD0Dlujg6rndIrD0Et3lnHlRDG0pEQ_A8HXHia</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1693598414</pqid></control><display><type>article</type><title>Quantification of Mirafiori lettuce big‐vein virus and its vector, Olpidium virulentus, from soil using real‐time PCR</title><source>Wiley Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library All Journals</source><creator>Momonoi, K ; Mori, M ; Matsuura, K ; Moriwaki, J ; Morikawa, T</creator><creatorcontrib>Momonoi, K ; Mori, M ; Matsuura, K ; Moriwaki, J ; Morikawa, T</creatorcontrib><description>Big vein disease of lettuce (Lactuca sativa) is an economically important disease transmitted through soil by Olpidium virulentus, and has occurred in most production areas worldwide. The disease is assumed to be caused by Mirafiori lettuce big‐vein virus (MiLBVV). To understand the dynamics of the virus and its vector, MiLBVV and O. virulentus were directly detected in soil. DNA and RNA were extracted from 5 g soil using a bead beating method, followed by purification using adsorption to a column. Detection and quantification were performed using real‐time PCR and a TaqMan probe that was prepared based on the CP region of MiLBVV and the rDNA‐ITS region of O. virulentus, respectively. Furthermore, using a visual assessment of the incidence rate of big vein disease on lettuce in agricultural fields, the Cₜ values of MiLBVV and O. virulentus from soil were also determined using real‐time PCR. The results showed that MiLBVV concentrations in the soil were high in the field, as also determined by a visual assessment of the incidence rate of big vein disease on lettuce. However, the amount of O. virulentus in soil was not directly correlated with the incidence of MiLBVV. From these results, it is suggested that the risk of lettuce crops developing big vein disease can be estimated using an index of the amount of MiLBVV in the soil.</description><identifier>ISSN: 0032-0862</identifier><identifier>EISSN: 1365-3059</identifier><identifier>DOI: 10.1111/ppa.12333</identifier><language>eng</language><publisher>Oxford: Blackwell Scientific Publications, etc</publisher><subject>adsorption ; crops ; DNA ; Lactuca sativa ; lettuce ; Lettuce big-vein associated virus ; Mirafiori lettuce big‐vein virus ; Olpidium ; Olpidium virulentus ; quantitative polymerase chain reaction ; real‐time PCR ; risk ; RNA ; soil ; viruses</subject><ispartof>Plant pathology, 2015-08, Vol.64 (4), p.825-830</ispartof><rights>2014 British Society for Plant Pathology</rights><rights>Plant Pathology © 2015 British Society for Plant Pathology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fppa.12333$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fppa.12333$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids></links><search><creatorcontrib>Momonoi, K</creatorcontrib><creatorcontrib>Mori, M</creatorcontrib><creatorcontrib>Matsuura, K</creatorcontrib><creatorcontrib>Moriwaki, J</creatorcontrib><creatorcontrib>Morikawa, T</creatorcontrib><title>Quantification of Mirafiori lettuce big‐vein virus and its vector, Olpidium virulentus, from soil using real‐time PCR</title><title>Plant pathology</title><description>Big vein disease of lettuce (Lactuca sativa) is an economically important disease transmitted through soil by Olpidium virulentus, and has occurred in most production areas worldwide. The disease is assumed to be caused by Mirafiori lettuce big‐vein virus (MiLBVV). To understand the dynamics of the virus and its vector, MiLBVV and O. virulentus were directly detected in soil. DNA and RNA were extracted from 5 g soil using a bead beating method, followed by purification using adsorption to a column. Detection and quantification were performed using real‐time PCR and a TaqMan probe that was prepared based on the CP region of MiLBVV and the rDNA‐ITS region of O. virulentus, respectively. Furthermore, using a visual assessment of the incidence rate of big vein disease on lettuce in agricultural fields, the Cₜ values of MiLBVV and O. virulentus from soil were also determined using real‐time PCR. The results showed that MiLBVV concentrations in the soil were high in the field, as also determined by a visual assessment of the incidence rate of big vein disease on lettuce. However, the amount of O. virulentus in soil was not directly correlated with the incidence of MiLBVV. From these results, it is suggested that the risk of lettuce crops developing big vein disease can be estimated using an index of the amount of MiLBVV in the soil.</description><subject>adsorption</subject><subject>crops</subject><subject>DNA</subject><subject>Lactuca sativa</subject><subject>lettuce</subject><subject>Lettuce big-vein associated virus</subject><subject>Mirafiori lettuce big‐vein virus</subject><subject>Olpidium</subject><subject>Olpidium virulentus</subject><subject>quantitative polymerase chain reaction</subject><subject>real‐time PCR</subject><subject>risk</subject><subject>RNA</subject><subject>soil</subject><subject>viruses</subject><issn>0032-0862</issn><issn>1365-3059</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNotkMtOwzAQRS0EEqWw4AuwxJa0fiSpvawqXlJRy6Nry03saqo0DrZT1B2fwDfyJaQts5kZzb13pIPQNSUD2tWwafSAMs75CepRnmcJJ5k8RT1COEuIyNk5ughhTQjNpBQ9tHttdR3BQqEjuBo7i1_AawvOA65MjG1h8BJWv98_WwM13oJvA9Z1iSEGvDVFdP4Oz6oGSmg3h3Nl6tiGO2y92-DgoMJtgHqFvdFVFxNhY_B88naJzqyugrn67320eLj_mDwl09nj82Q8TWxKBU9yTkmWi0zSNLUmL3JhS1YYSZe2YFKS5YgZbtOC04zITFuhmew2bTRPS1KmvI9uj7mNd5-tCVGtXevr7qWiueSZFCndq4ZH1RdUZqcaDxvtd4oStceqOqzqgFXN5-PD0Dlujg6rndIrD0Et3lnHlRDG0pEQ_A8HXHia</recordid><startdate>201508</startdate><enddate>201508</enddate><creator>Momonoi, K</creator><creator>Mori, M</creator><creator>Matsuura, K</creator><creator>Moriwaki, J</creator><creator>Morikawa, T</creator><general>Blackwell Scientific Publications, etc</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201508</creationdate><title>Quantification of Mirafiori lettuce big‐vein virus and its vector, Olpidium virulentus, from soil using real‐time PCR</title><author>Momonoi, K ; Mori, M ; Matsuura, K ; Moriwaki, J ; Morikawa, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f4183-631056859144fe6c68fd2ce91bfc2990b72e3f4c315095af8a294c3aea34d0d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>adsorption</topic><topic>crops</topic><topic>DNA</topic><topic>Lactuca sativa</topic><topic>lettuce</topic><topic>Lettuce big-vein associated virus</topic><topic>Mirafiori lettuce big‐vein virus</topic><topic>Olpidium</topic><topic>Olpidium virulentus</topic><topic>quantitative polymerase chain reaction</topic><topic>real‐time PCR</topic><topic>risk</topic><topic>RNA</topic><topic>soil</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Momonoi, K</creatorcontrib><creatorcontrib>Mori, M</creatorcontrib><creatorcontrib>Matsuura, K</creatorcontrib><creatorcontrib>Moriwaki, J</creatorcontrib><creatorcontrib>Morikawa, T</creatorcontrib><collection>AGRIS</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Momonoi, K</au><au>Mori, M</au><au>Matsuura, K</au><au>Moriwaki, J</au><au>Morikawa, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of Mirafiori lettuce big‐vein virus and its vector, Olpidium virulentus, from soil using real‐time PCR</atitle><jtitle>Plant pathology</jtitle><date>2015-08</date><risdate>2015</risdate><volume>64</volume><issue>4</issue><spage>825</spage><epage>830</epage><pages>825-830</pages><issn>0032-0862</issn><eissn>1365-3059</eissn><abstract>Big vein disease of lettuce (Lactuca sativa) is an economically important disease transmitted through soil by Olpidium virulentus, and has occurred in most production areas worldwide. The disease is assumed to be caused by Mirafiori lettuce big‐vein virus (MiLBVV). To understand the dynamics of the virus and its vector, MiLBVV and O. virulentus were directly detected in soil. DNA and RNA were extracted from 5 g soil using a bead beating method, followed by purification using adsorption to a column. Detection and quantification were performed using real‐time PCR and a TaqMan probe that was prepared based on the CP region of MiLBVV and the rDNA‐ITS region of O. virulentus, respectively. Furthermore, using a visual assessment of the incidence rate of big vein disease on lettuce in agricultural fields, the Cₜ values of MiLBVV and O. virulentus from soil were also determined using real‐time PCR. The results showed that MiLBVV concentrations in the soil were high in the field, as also determined by a visual assessment of the incidence rate of big vein disease on lettuce. However, the amount of O. virulentus in soil was not directly correlated with the incidence of MiLBVV. From these results, it is suggested that the risk of lettuce crops developing big vein disease can be estimated using an index of the amount of MiLBVV in the soil.</abstract><cop>Oxford</cop><pub>Blackwell Scientific Publications, etc</pub><doi>10.1111/ppa.12333</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0032-0862
ispartof Plant pathology, 2015-08, Vol.64 (4), p.825-830
issn 0032-0862
1365-3059
language eng
recordid cdi_proquest_journals_1693598414
source Wiley Free Content; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals
subjects adsorption
crops
DNA
Lactuca sativa
lettuce
Lettuce big-vein associated virus
Mirafiori lettuce big‐vein virus
Olpidium
Olpidium virulentus
quantitative polymerase chain reaction
real‐time PCR
risk
RNA
soil
viruses
title Quantification of Mirafiori lettuce big‐vein virus and its vector, Olpidium virulentus, from soil using real‐time PCR
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T12%3A08%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_wiley&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantification%20of%20Mirafiori%20lettuce%20big%E2%80%90vein%20virus%20and%20its%20vector,%20Olpidium%20virulentus,%20from%20soil%20using%20real%E2%80%90time%20PCR&rft.jtitle=Plant%20pathology&rft.au=Momonoi,%20K&rft.date=2015-08&rft.volume=64&rft.issue=4&rft.spage=825&rft.epage=830&rft.pages=825-830&rft.issn=0032-0862&rft.eissn=1365-3059&rft_id=info:doi/10.1111/ppa.12333&rft_dat=%3Cproquest_wiley%3E3734842631%3C/proquest_wiley%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1693598414&rft_id=info:pmid/&rfr_iscdi=true