Diverse activity of human secretory phospholipases A^sub 2^ on the migration of human vascular smooth muscle cells

Investigation of the diversity of human secretory phospholipases A^sub 2^ (sPLA^sub 2^) on the migration of human vascular smooth muscle cells (VSMC). We investigated the impact of sPLA^sub 2^ IIA, V, and X and of oleic acid, linoleic acid and lysophosphatidylcholine on the migration of human VSMC. Recombinant human sPLA^sub 2^'s and Boyden's chamber method were applied. sPLA^sub 2,^ IIA but not V or X enhanced migration of VSMC in a dose/time dependent manner. Oleic and linoleic acids, and lysophosphatidylcholine markedly enhanced migration. These results imply that sPLA^sub 2^ IIA, which is known to be present in the arterial wall in the vicinity of VSMC, as well as products of lipid hydrolysis induced by sPLA^sub 2,^ enhance the migration of VSMC, and thus may contribute to atherogenic process.