Estandarización de un protocolo sencillo para la extracción de ADN genómico de levaduras

A quick, simple and low-cost protocol for extracting genomic DNA from yeast by cell wall lysis involving enzymatic treatment and alcoholic precipitation was standardised. Higher DNA yields (124.9±30.2 ng/μl) were obtained by using beta-glucuronidase instead of zymolyase; these had very high quality...

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Veröffentlicht in:Revista Colombiana de biotecnologia 2009-01, Vol.11 (1), p.125
Hauptverfasser: Osorio-Cadavid, Esteban, Ramírez, Mauricio, William Andrés López, Luz Adriana Mambuscay
Format: Artikel
Sprache:eng ; spa
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