Organogénesis directa in vitro a partir de hojas de la planta Antiplasmodial Solanum nudum Dunal

Solanum nudum Dunal (Solanaceae) is a plant with a potential for developing chemotherapeutic treatments against malaria. This is the first report of a fast, efficient, and reproducible direct organogenesis protocol from leave segments from in vitro seed-grown plantlets. Leaves segments from 5 months...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Revista Colombiana de biotecnologia 2011-07, Vol.13 (2), p.186
Hauptverfasser: Suárez Cardoso, Oscar Manuel, Naranjo Gómez, Esther Julia, Garcés, Lucía Atehortúa, Silvia Blair Trujillo
Format: Artikel
Sprache:eng ; spa
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Solanum nudum Dunal (Solanaceae) is a plant with a potential for developing chemotherapeutic treatments against malaria. This is the first report of a fast, efficient, and reproducible direct organogenesis protocol from leave segments from in vitro seed-grown plantlets. Leaves segments from 5 months old germinated plantlets were placed on half concentration Murashige and Skoog medium (MS), supplemented with several concentrations of Bencilaminopurin (BAP) combinated with Indolacetic Acid (IAA). Dark/ light incubation effect in periods 0/45, 15/30 and 30/15 dark/light days were evaluated on the buds induction. High frequency buds formation was shown (4,83) in explants cultured on MS supplemented with BAP 2,0 mg/L and AIA 0,1 mg/L under a period of 30 days of dark condition incubation. After induction, buds obtained were transferred to MS medium supplemented with gibberellic acid (GA3) 0,3 mg/L and maintained under artificial cool light, there the plantlets rooted. Regenerated plantlets were placed under greenhouse conditions and these were morphologically similar to donor plants.
ISSN:0123-3475
1909-8758