Effects of the endophyte Epichloë festucae var. lolii of perennial ryegrass (Lolium perenne) on indicators of oxidative stress from pathogenic fungi during seed germination and seedling growth
To determine whether infection by a fungal endophyte of the genus Epichloë has a modifying influence on pathogenic fungal stress, endophyte-infected (E+) and endophyte-free (E-) seeds of perennial ryegrass (Lolium perenne), inoculated with the ryegrass pathogens Alternaria alternata, Ascochyta lepto...
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| Veröffentlicht in: | European journal of plant pathology 2015-03, Vol.141 (3), p.571-583 |
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| creator | Ma, Minzhi Christensen, Michael J Nan, Zhibiao |
| description | To determine whether infection by a fungal endophyte of the genus Epichloë has a modifying influence on pathogenic fungal stress, endophyte-infected (E+) and endophyte-free (E-) seeds of perennial ryegrass (Lolium perenne), inoculated with the ryegrass pathogens Alternaria alternata, Ascochyta leptospora, Bipolaris sorokiniana, Curvularia lunata and Fusarium avenaceum were used for germination experiments and quantification of oxidative stress. Four measures of oxidative stress were used: malonaldehyde (MDA) content, peroxidases (POD) and superoxide dismutase (SOD) activity, and proline content. MDA content, an indicator of membrane lipid peroxidation damage, was significantly lower in E+ seedlings than in E- seedlings under stress from the test fungi. POD activity of E+ seedlings exposed to A. alternata, B. sorokiniana and C. lunata was significantly higher than similarly challenged E- seedlings. SOD activity of E+ seedlings was significantly higher than E- seedlings after exposure to A. alternata, B. sorokiniana, C. lunata and As. leptospora. Proline content of E+ seedlings exposed to the five pathogens was significantly higher than E- seedlings. Interestingly, these effects were present in the absence of disease symptoms apart from some root browning of E+ and E- seedlings exposed to B. sorokiniana. The results of this study showed that the seed-borne endophyte E. festucae var. lolii has potential to enhance establishment of perennial ryegrass by providing protection against pathogenic fungi during the critical early stages of seed germination and subsequent growth. |
| doi_str_mv | 10.1007/s10658-014-0563-x |
| format | Article |
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Four measures of oxidative stress were used: malonaldehyde (MDA) content, peroxidases (POD) and superoxide dismutase (SOD) activity, and proline content. MDA content, an indicator of membrane lipid peroxidation damage, was significantly lower in E+ seedlings than in E- seedlings under stress from the test fungi. POD activity of E+ seedlings exposed to A. alternata, B. sorokiniana and C. lunata was significantly higher than similarly challenged E- seedlings. SOD activity of E+ seedlings was significantly higher than E- seedlings after exposure to A. alternata, B. sorokiniana, C. lunata and As. leptospora. Proline content of E+ seedlings exposed to the five pathogens was significantly higher than E- seedlings. Interestingly, these effects were present in the absence of disease symptoms apart from some root browning of E+ and E- seedlings exposed to B. sorokiniana. The results of this study showed that the seed-borne endophyte E. festucae var. lolii has potential to enhance establishment of perennial ryegrass by providing protection against pathogenic fungi during the critical early stages of seed germination and subsequent growth.</description><identifier>ISSN: 0929-1873</identifier><identifier>EISSN: 1573-8469</identifier><identifier>DOI: 10.1007/s10658-014-0563-x</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Agriculture ; Alternaria alternata ; Ascochyta ; Biomedical and Life Sciences ; Bipolaris sorokiniana ; Curvularia lunata ; Ecology ; endophytes ; Fungi ; Fusarium avenaceum ; Germination ; Life Sciences ; lipid peroxidation ; Lolium perenne ; malondialdehyde ; Oxidative stress ; Pathogens ; peroxidase ; Peroxidation ; Plant growth ; Plant Pathology ; Plant Sciences ; proline ; Seed germination ; seedling growth ; Seedlings ; Seeds ; superoxide dismutase</subject><ispartof>European journal of plant pathology, 2015-03, Vol.141 (3), p.571-583</ispartof><rights>Koninklijke Nederlandse Planteziektenkundige Vereniging 2014</rights><rights>Koninklijke Nederlandse Planteziektenkundige Vereniging 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-61eab4e11e56d1ab6f743528f8e6e1425aeae4d073e3a30e4f1898e0b87641773</citedby><cites>FETCH-LOGICAL-c340t-61eab4e11e56d1ab6f743528f8e6e1425aeae4d073e3a30e4f1898e0b87641773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10658-014-0563-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10658-014-0563-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Ma, Minzhi</creatorcontrib><creatorcontrib>Christensen, Michael J</creatorcontrib><creatorcontrib>Nan, Zhibiao</creatorcontrib><title>Effects of the endophyte Epichloë festucae var. lolii of perennial ryegrass (Lolium perenne) on indicators of oxidative stress from pathogenic fungi during seed germination and seedling growth</title><title>European journal of plant pathology</title><addtitle>Eur J Plant Pathol</addtitle><description>To determine whether infection by a fungal endophyte of the genus Epichloë has a modifying influence on pathogenic fungal stress, endophyte-infected (E+) and endophyte-free (E-) seeds of perennial ryegrass (Lolium perenne), inoculated with the ryegrass pathogens Alternaria alternata, Ascochyta leptospora, Bipolaris sorokiniana, Curvularia lunata and Fusarium avenaceum were used for germination experiments and quantification of oxidative stress. Four measures of oxidative stress were used: malonaldehyde (MDA) content, peroxidases (POD) and superoxide dismutase (SOD) activity, and proline content. MDA content, an indicator of membrane lipid peroxidation damage, was significantly lower in E+ seedlings than in E- seedlings under stress from the test fungi. POD activity of E+ seedlings exposed to A. alternata, B. sorokiniana and C. lunata was significantly higher than similarly challenged E- seedlings. SOD activity of E+ seedlings was significantly higher than E- seedlings after exposure to A. alternata, B. sorokiniana, C. lunata and As. leptospora. Proline content of E+ seedlings exposed to the five pathogens was significantly higher than E- seedlings. Interestingly, these effects were present in the absence of disease symptoms apart from some root browning of E+ and E- seedlings exposed to B. sorokiniana. The results of this study showed that the seed-borne endophyte E. festucae var. lolii has potential to enhance establishment of perennial ryegrass by providing protection against pathogenic fungi during the critical early stages of seed germination and subsequent growth.</description><subject>Agriculture</subject><subject>Alternaria alternata</subject><subject>Ascochyta</subject><subject>Biomedical and Life Sciences</subject><subject>Bipolaris sorokiniana</subject><subject>Curvularia lunata</subject><subject>Ecology</subject><subject>endophytes</subject><subject>Fungi</subject><subject>Fusarium avenaceum</subject><subject>Germination</subject><subject>Life Sciences</subject><subject>lipid peroxidation</subject><subject>Lolium perenne</subject><subject>malondialdehyde</subject><subject>Oxidative stress</subject><subject>Pathogens</subject><subject>peroxidase</subject><subject>Peroxidation</subject><subject>Plant growth</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>proline</subject><subject>Seed germination</subject><subject>seedling growth</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>superoxide dismutase</subject><issn>0929-1873</issn><issn>1573-8469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kcFu1DAURSMEEkPhA1hhiQ0s0vrFjpMsUTW0lUZiAV1bnuQ5cZWxg-20M9_EV6D-WJ2mC1asnvTuPffJvln2Eeg5UFpdBKCirHMKPKelYPnxVbaBsmJ5zUXzOtvQpmhyqCv2NnsXwh1NTNMUm-zvVmtsYyBOkzggQdu5aThFJNvJtMPoHv8QjSHOrUJyr_w5Gd1ozGKf0KO1Ro3En7D3KgTyZZfE-fAi4VfiLDG2M62Kzj_fcEfTqWjukYToMSHau-RXcXA9WtMSPdvekG72xvYkIHakR38wNkEpTNnueTkuau_dQxzeZ2-0GgN-eJln2e337a_L63z34-rm8tsubxmnMReAas8RAEvRgdoLXXFWFrWuUSDwolSokHe0YsgUo8g11E2NdF9XgkNVsbPs85o7efd7Tl8i79zsbTopQZSUNdDUNLlgdbXeheBRy8mbg_InCVQuTcm1KZmakktT8piYYmXCtLwa_T_J_4E-rZBWTqremyBvfxYUSkqhYVxU7AkZgqVT</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Ma, Minzhi</creator><creator>Christensen, Michael J</creator><creator>Nan, Zhibiao</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X2</scope><scope>88A</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20150301</creationdate><title>Effects of the endophyte Epichloë festucae var. lolii of perennial ryegrass (Lolium perenne) on indicators of oxidative stress from pathogenic fungi during seed germination and seedling growth</title><author>Ma, Minzhi ; Christensen, Michael J ; Nan, Zhibiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-61eab4e11e56d1ab6f743528f8e6e1425aeae4d073e3a30e4f1898e0b87641773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Agriculture</topic><topic>Alternaria alternata</topic><topic>Ascochyta</topic><topic>Biomedical and Life Sciences</topic><topic>Bipolaris sorokiniana</topic><topic>Curvularia lunata</topic><topic>Ecology</topic><topic>endophytes</topic><topic>Fungi</topic><topic>Fusarium avenaceum</topic><topic>Germination</topic><topic>Life Sciences</topic><topic>lipid peroxidation</topic><topic>Lolium perenne</topic><topic>malondialdehyde</topic><topic>Oxidative stress</topic><topic>Pathogens</topic><topic>peroxidase</topic><topic>Peroxidation</topic><topic>Plant growth</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>proline</topic><topic>Seed germination</topic><topic>seedling growth</topic><topic>Seedlings</topic><topic>Seeds</topic><topic>superoxide dismutase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Minzhi</creatorcontrib><creatorcontrib>Christensen, Michael J</creatorcontrib><creatorcontrib>Nan, Zhibiao</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Databases</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Agricultural Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>European journal of plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Minzhi</au><au>Christensen, Michael J</au><au>Nan, Zhibiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of the endophyte Epichloë festucae var. lolii of perennial ryegrass (Lolium perenne) on indicators of oxidative stress from pathogenic fungi during seed germination and seedling growth</atitle><jtitle>European journal of plant pathology</jtitle><stitle>Eur J Plant Pathol</stitle><date>2015-03-01</date><risdate>2015</risdate><volume>141</volume><issue>3</issue><spage>571</spage><epage>583</epage><pages>571-583</pages><issn>0929-1873</issn><eissn>1573-8469</eissn><abstract>To determine whether infection by a fungal endophyte of the genus Epichloë has a modifying influence on pathogenic fungal stress, endophyte-infected (E+) and endophyte-free (E-) seeds of perennial ryegrass (Lolium perenne), inoculated with the ryegrass pathogens Alternaria alternata, Ascochyta leptospora, Bipolaris sorokiniana, Curvularia lunata and Fusarium avenaceum were used for germination experiments and quantification of oxidative stress. Four measures of oxidative stress were used: malonaldehyde (MDA) content, peroxidases (POD) and superoxide dismutase (SOD) activity, and proline content. MDA content, an indicator of membrane lipid peroxidation damage, was significantly lower in E+ seedlings than in E- seedlings under stress from the test fungi. POD activity of E+ seedlings exposed to A. alternata, B. sorokiniana and C. lunata was significantly higher than similarly challenged E- seedlings. SOD activity of E+ seedlings was significantly higher than E- seedlings after exposure to A. alternata, B. sorokiniana, C. lunata and As. leptospora. Proline content of E+ seedlings exposed to the five pathogens was significantly higher than E- seedlings. Interestingly, these effects were present in the absence of disease symptoms apart from some root browning of E+ and E- seedlings exposed to B. sorokiniana. The results of this study showed that the seed-borne endophyte E. festucae var. lolii has potential to enhance establishment of perennial ryegrass by providing protection against pathogenic fungi during the critical early stages of seed germination and subsequent growth.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><doi>10.1007/s10658-014-0563-x</doi><tpages>13</tpages></addata></record> |
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| subjects | Agriculture Alternaria alternata Ascochyta Biomedical and Life Sciences Bipolaris sorokiniana Curvularia lunata Ecology endophytes Fungi Fusarium avenaceum Germination Life Sciences lipid peroxidation Lolium perenne malondialdehyde Oxidative stress Pathogens peroxidase Peroxidation Plant growth Plant Pathology Plant Sciences proline Seed germination seedling growth Seedlings Seeds superoxide dismutase |
| title | Effects of the endophyte Epichloë festucae var. lolii of perennial ryegrass (Lolium perenne) on indicators of oxidative stress from pathogenic fungi during seed germination and seedling growth |
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