Determination of Antibody Responses to MPB83 Antigen in BCG-vaccinated Rusa Deer (Rusa timorensis)

Bovine tuberculosis (BTB) is a contagious zoonotic disease that causes serious public health concerns. Cervidae are highly susceptible to BTB and it has been diagnosed in various deer species. In order to develop BTB eradication and control program, Mycobacterium bovis Bacille Calmette Guerin (BCG)...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Wētchasān sattawaphāet 2014-12, Vol.44 (4), p.407
Hauptverfasser: Chaichanathong, Sirinart, Wajjwalku, Worawidh, Prasanwong, Chayanid, Suksamranthaweerat, Melanee, Rasitanon, Piyawan, Phojun, Aunchisa, Watanangura, Antja, Boodde, Orawan, Thongtip, Nikorn
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 4
container_start_page 407
container_title Wētchasān sattawaphāet
container_volume 44
creator Chaichanathong, Sirinart
Wajjwalku, Worawidh
Prasanwong, Chayanid
Suksamranthaweerat, Melanee
Rasitanon, Piyawan
Phojun, Aunchisa
Watanangura, Antja
Boodde, Orawan
Thongtip, Nikorn
description Bovine tuberculosis (BTB) is a contagious zoonotic disease that causes serious public health concerns. Cervidae are highly susceptible to BTB and it has been diagnosed in various deer species. In order to develop BTB eradication and control program, Mycobacterium bovis Bacille Calmette Guerin (BCG) vaccination has been studied in cervids. In this study, 15 rusa deer were tested for responsiveness to mycobacterial reaction using a comparative cervical skin test. The rusa deer were divided into 2 groups; BCG vaccination group, which were inoculated with a single dose of 2x10^sup 6^ cfu M. bovis BCG Tokyo subcutaneously and control group. The MPB83 protein was constructed and used as a specific antigen for an indirect ELISA to determine antibody levels against BCG vaccination in rusa deer. Sera were collected pre-vaccination and 4, 10, 15, 20 and 25 weeks post-vaccination. The antibody levels at each time point were determined by indirect ELISA using the MPB83 antigen. In the vaccination group, mean difference in optical density increased significantly 4 weeks after vaccination.
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_1649791626</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3575108341</sourcerecordid><originalsourceid>FETCH-LOGICAL-p183t-c2e74173c8b1791e5ce4a9e67c621a0b67311118c6a34cf390c0613ade710cd73</originalsourceid><addsrcrecordid>eNotT1FLwzAYzIOCY-4_BHzRh0K-JE3ax63TOZgoQ59Hmn6VDJfUJBX899bpvdzBcXfcBZkx4GWhZA1XZJHSkTEGrJRM8Blp15gxnpw32QVPQ0-XPrs2dN90j2kIPmGiOdCnl1Ulzt47euo8XTWb4stY-5vEju7HZOgaMdLbs8zuFCL65NLdNbnszUfCxT_PydvD_WvzWOyeN9tmuSsGqEQuLEctQQtbtaBrwNKiNDUqbRUHw1qlBUyorDJC2l7UzDIFwnSogdlOizm5-esdYvgcMeXDMYzRT5MHmM5PnYor8QMlKE9k</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1649791626</pqid></control><display><type>article</type><title>Determination of Antibody Responses to MPB83 Antigen in BCG-vaccinated Rusa Deer (Rusa timorensis)</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Chaichanathong, Sirinart ; Wajjwalku, Worawidh ; Prasanwong, Chayanid ; Suksamranthaweerat, Melanee ; Rasitanon, Piyawan ; Phojun, Aunchisa ; Watanangura, Antja ; Boodde, Orawan ; Thongtip, Nikorn</creator><creatorcontrib>Chaichanathong, Sirinart ; Wajjwalku, Worawidh ; Prasanwong, Chayanid ; Suksamranthaweerat, Melanee ; Rasitanon, Piyawan ; Phojun, Aunchisa ; Watanangura, Antja ; Boodde, Orawan ; Thongtip, Nikorn</creatorcontrib><description>Bovine tuberculosis (BTB) is a contagious zoonotic disease that causes serious public health concerns. Cervidae are highly susceptible to BTB and it has been diagnosed in various deer species. In order to develop BTB eradication and control program, Mycobacterium bovis Bacille Calmette Guerin (BCG) vaccination has been studied in cervids. In this study, 15 rusa deer were tested for responsiveness to mycobacterial reaction using a comparative cervical skin test. The rusa deer were divided into 2 groups; BCG vaccination group, which were inoculated with a single dose of 2x10^sup 6^ cfu M. bovis BCG Tokyo subcutaneously and control group. The MPB83 protein was constructed and used as a specific antigen for an indirect ELISA to determine antibody levels against BCG vaccination in rusa deer. Sera were collected pre-vaccination and 4, 10, 15, 20 and 25 weeks post-vaccination. The antibody levels at each time point were determined by indirect ELISA using the MPB83 antigen. In the vaccination group, mean difference in optical density increased significantly 4 weeks after vaccination.</description><identifier>ISSN: 0125-6491</identifier><language>eng</language><publisher>Bangkok: Chulalongkorn University, Faculty of Veterinary Science</publisher><subject>Agricultural biotechnology ; Animals ; Antigens ; Bacteria ; Cattle ; Cloning ; Elk ; Genetic engineering ; Infections ; Laboratories ; Plasmids ; Protein expression ; Proteins ; Studies ; Tuberculosis ; Zoonoses</subject><ispartof>Wētchasān sattawaphāet, 2014-12, Vol.44 (4), p.407</ispartof><rights>Copyright Chulalongkorn University, Faculty of Veterinary Science Dec 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Chaichanathong, Sirinart</creatorcontrib><creatorcontrib>Wajjwalku, Worawidh</creatorcontrib><creatorcontrib>Prasanwong, Chayanid</creatorcontrib><creatorcontrib>Suksamranthaweerat, Melanee</creatorcontrib><creatorcontrib>Rasitanon, Piyawan</creatorcontrib><creatorcontrib>Phojun, Aunchisa</creatorcontrib><creatorcontrib>Watanangura, Antja</creatorcontrib><creatorcontrib>Boodde, Orawan</creatorcontrib><creatorcontrib>Thongtip, Nikorn</creatorcontrib><title>Determination of Antibody Responses to MPB83 Antigen in BCG-vaccinated Rusa Deer (Rusa timorensis)</title><title>Wētchasān sattawaphāet</title><description>Bovine tuberculosis (BTB) is a contagious zoonotic disease that causes serious public health concerns. Cervidae are highly susceptible to BTB and it has been diagnosed in various deer species. In order to develop BTB eradication and control program, Mycobacterium bovis Bacille Calmette Guerin (BCG) vaccination has been studied in cervids. In this study, 15 rusa deer were tested for responsiveness to mycobacterial reaction using a comparative cervical skin test. The rusa deer were divided into 2 groups; BCG vaccination group, which were inoculated with a single dose of 2x10^sup 6^ cfu M. bovis BCG Tokyo subcutaneously and control group. The MPB83 protein was constructed and used as a specific antigen for an indirect ELISA to determine antibody levels against BCG vaccination in rusa deer. Sera were collected pre-vaccination and 4, 10, 15, 20 and 25 weeks post-vaccination. The antibody levels at each time point were determined by indirect ELISA using the MPB83 antigen. In the vaccination group, mean difference in optical density increased significantly 4 weeks after vaccination.</description><subject>Agricultural biotechnology</subject><subject>Animals</subject><subject>Antigens</subject><subject>Bacteria</subject><subject>Cattle</subject><subject>Cloning</subject><subject>Elk</subject><subject>Genetic engineering</subject><subject>Infections</subject><subject>Laboratories</subject><subject>Plasmids</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Studies</subject><subject>Tuberculosis</subject><subject>Zoonoses</subject><issn>0125-6491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNotT1FLwzAYzIOCY-4_BHzRh0K-JE3ax63TOZgoQ59Hmn6VDJfUJBX899bpvdzBcXfcBZkx4GWhZA1XZJHSkTEGrJRM8Blp15gxnpw32QVPQ0-XPrs2dN90j2kIPmGiOdCnl1Ulzt47euo8XTWb4stY-5vEju7HZOgaMdLbs8zuFCL65NLdNbnszUfCxT_PydvD_WvzWOyeN9tmuSsGqEQuLEctQQtbtaBrwNKiNDUqbRUHw1qlBUyorDJC2l7UzDIFwnSogdlOizm5-esdYvgcMeXDMYzRT5MHmM5PnYor8QMlKE9k</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Chaichanathong, Sirinart</creator><creator>Wajjwalku, Worawidh</creator><creator>Prasanwong, Chayanid</creator><creator>Suksamranthaweerat, Melanee</creator><creator>Rasitanon, Piyawan</creator><creator>Phojun, Aunchisa</creator><creator>Watanangura, Antja</creator><creator>Boodde, Orawan</creator><creator>Thongtip, Nikorn</creator><general>Chulalongkorn University, Faculty of Veterinary Science</general><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BVBZV</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20141201</creationdate><title>Determination of Antibody Responses to MPB83 Antigen in BCG-vaccinated Rusa Deer (Rusa timorensis)</title><author>Chaichanathong, Sirinart ; Wajjwalku, Worawidh ; Prasanwong, Chayanid ; Suksamranthaweerat, Melanee ; Rasitanon, Piyawan ; Phojun, Aunchisa ; Watanangura, Antja ; Boodde, Orawan ; Thongtip, Nikorn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p183t-c2e74173c8b1791e5ce4a9e67c621a0b67311118c6a34cf390c0613ade710cd73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Agricultural biotechnology</topic><topic>Animals</topic><topic>Antigens</topic><topic>Bacteria</topic><topic>Cattle</topic><topic>Cloning</topic><topic>Elk</topic><topic>Genetic engineering</topic><topic>Infections</topic><topic>Laboratories</topic><topic>Plasmids</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Studies</topic><topic>Tuberculosis</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaichanathong, Sirinart</creatorcontrib><creatorcontrib>Wajjwalku, Worawidh</creatorcontrib><creatorcontrib>Prasanwong, Chayanid</creatorcontrib><creatorcontrib>Suksamranthaweerat, Melanee</creatorcontrib><creatorcontrib>Rasitanon, Piyawan</creatorcontrib><creatorcontrib>Phojun, Aunchisa</creatorcontrib><creatorcontrib>Watanangura, Antja</creatorcontrib><creatorcontrib>Boodde, Orawan</creatorcontrib><creatorcontrib>Thongtip, Nikorn</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>East &amp; South Asia Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Wētchasān sattawaphāet</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaichanathong, Sirinart</au><au>Wajjwalku, Worawidh</au><au>Prasanwong, Chayanid</au><au>Suksamranthaweerat, Melanee</au><au>Rasitanon, Piyawan</au><au>Phojun, Aunchisa</au><au>Watanangura, Antja</au><au>Boodde, Orawan</au><au>Thongtip, Nikorn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Antibody Responses to MPB83 Antigen in BCG-vaccinated Rusa Deer (Rusa timorensis)</atitle><jtitle>Wētchasān sattawaphāet</jtitle><date>2014-12-01</date><risdate>2014</risdate><volume>44</volume><issue>4</issue><spage>407</spage><pages>407-</pages><issn>0125-6491</issn><abstract>Bovine tuberculosis (BTB) is a contagious zoonotic disease that causes serious public health concerns. Cervidae are highly susceptible to BTB and it has been diagnosed in various deer species. In order to develop BTB eradication and control program, Mycobacterium bovis Bacille Calmette Guerin (BCG) vaccination has been studied in cervids. In this study, 15 rusa deer were tested for responsiveness to mycobacterial reaction using a comparative cervical skin test. The rusa deer were divided into 2 groups; BCG vaccination group, which were inoculated with a single dose of 2x10^sup 6^ cfu M. bovis BCG Tokyo subcutaneously and control group. The MPB83 protein was constructed and used as a specific antigen for an indirect ELISA to determine antibody levels against BCG vaccination in rusa deer. Sera were collected pre-vaccination and 4, 10, 15, 20 and 25 weeks post-vaccination. The antibody levels at each time point were determined by indirect ELISA using the MPB83 antigen. In the vaccination group, mean difference in optical density increased significantly 4 weeks after vaccination.</abstract><cop>Bangkok</cop><pub>Chulalongkorn University, Faculty of Veterinary Science</pub></addata></record>
fulltext fulltext
identifier ISSN: 0125-6491
ispartof Wētchasān sattawaphāet, 2014-12, Vol.44 (4), p.407
issn 0125-6491
language eng
recordid cdi_proquest_journals_1649791626
source EZB-FREE-00999 freely available EZB journals
subjects Agricultural biotechnology
Animals
Antigens
Bacteria
Cattle
Cloning
Elk
Genetic engineering
Infections
Laboratories
Plasmids
Protein expression
Proteins
Studies
Tuberculosis
Zoonoses
title Determination of Antibody Responses to MPB83 Antigen in BCG-vaccinated Rusa Deer (Rusa timorensis)
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T01%3A39%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20Antibody%20Responses%20to%20MPB83%20Antigen%20in%20BCG-vaccinated%20Rusa%20Deer%20(Rusa%20timorensis)&rft.jtitle=W%C4%93tchas%C4%81n%20sattawaph%C4%81et&rft.au=Chaichanathong,%20Sirinart&rft.date=2014-12-01&rft.volume=44&rft.issue=4&rft.spage=407&rft.pages=407-&rft.issn=0125-6491&rft_id=info:doi/&rft_dat=%3Cproquest%3E3575108341%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1649791626&rft_id=info:pmid/&rfr_iscdi=true