The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5Å resolution
The enzymatic degradation of plant cell-wall cellulose is central to many industrial processes, including second-generation biofuel production. Key players in this deconstruction are the fungal cellobiohydrolases (CBHs), notably those from family GH7 of the carbohydrate-active enzymes (CAZY) databas...
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| Veröffentlicht in: | Acta crystallographica. Section F, Structural biology communications Structural biology communications, 2015-01, Vol.71 (1), p.114 |
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| container_title | Acta crystallographica. Section F, Structural biology communications |
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| creator | Moroz, Olga V Maranta, Michelle Shaghasi, Tarana Harris, Paul V Wilson, Keith S Davies, Gideon J |
| description | The enzymatic degradation of plant cell-wall cellulose is central to many industrial processes, including second-generation biofuel production. Key players in this deconstruction are the fungal cellobiohydrolases (CBHs), notably those from family GH7 of the carbohydrate-active enzymes (CAZY) database, which are generally known as CBHI enzymes. Here, three-dimensional structures are reported of the Aspergillus fumigatus CBHI Cel7A solved in uncomplexed and disaccharide-bound forms at resolutions of 1.8 and 1.5Å, respectively. The product complex with a disaccharide in the +1 and +2 subsites adds to the growing three-dimensional insight into this family of industrially relevant biocatalysts. |
| doi_str_mv | 10.1107/S2053230X14027307 |
| format | Article |
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| source | Wiley Online Library Journals Frontfile Complete; PubMed Central; Free Full-Text Journals in Chemistry |
| title | The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5Å resolution |
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