Diels-Alder reaction–triggered bioorthogonal protein decaging in living cells

Diels-Alder chemistry is widely used for bioconjugations, and one variant of the reaction can ‘deprotect’ a small molecule via spontaneous elimination. This activation chemistry is now demonstrated on biomolecules in cells at high yields in 10 minutes. Small molecules that specifically activate an i...

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Veröffentlicht in:	Nature chemical biology 2014-12, Vol.10 (12), p.1003-1005
Hauptverfasser:	Li, Jie, Jia, Shang, Chen, Peng R
Format:	Artikel
Sprache:	eng
Schlagworte:	14/19 38/5 631/80 631/92/2783 631/92/612 631/92/96 82/29 82/58 82/80 82/83 Biochemical Engineering Biochemistry Bioorganic Chemistry brief-communication Cell Biology Chemistry Chemistry/Food Science Cycloaddition Reaction Electrons Enzyme Activation Enzymes Escherichia coli - genetics Escherichia coli - metabolism Gene Expression Genes, Reporter HEK293 Cells Humans Luciferases - chemistry Luciferases - genetics Luciferases - metabolism Luminescent Measurements Lysine - chemistry Molecular Conformation Molecules Plasmids Proteins Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Small Molecule Libraries - chemistry Staining and Labeling - methods Tetrazoles - chemistry
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