Qualitative determination of bioactive metabolites through Q-TOF LC/MS in different parts and undifferentiated cultures of Ulmus wallichiana Planchon
Ulmus wallichiana is an endangered and folk medicinal plant used for healing fractures in India. Recently, the secondary metabolites isolated and identified from its bark revealed osteogenic activity. Considering the medicinal properties of its bark and the source for its identified and isolated met...
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| Veröffentlicht in: | Plant growth regulation 2015-01, Vol.75 (1), p.331-340 |
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| creator | Kumari, Tejaswita Sharma, Chetan Bajpai, Vikas Kumar, Brijesh Srivastava, Mukesh Arya, K. R |
| description | Ulmus wallichiana is an endangered and folk medicinal plant used for healing fractures in India. Recently, the secondary metabolites isolated and identified from its bark revealed osteogenic activity. Considering the medicinal properties of its bark and the source for its identified and isolated metabolites, the requirement for production of alternative pathways was crucial. This study aimed to determine its bioactive metabolite using Quadrupole time-of-flight liquid chromatography-mass spectroscopy (Q-TOF LC/MS) in different parts of this plant with seasonal and altitudinal variability pertaining with the development of a protocol for induction of callus cultures and biomass production. Plant samples were collected during December–March, April–July and August–November in the year 2010–2012 from three different altitudinal locations (Nainital, Almora and Bageshwar districts) of Kumaon region, Uttarakhand, India. The results obtained from Q-TOF LC/MS analysis demonstrated the presence of four bioactive metabolites known for osteogenic activity in bark and stem samples round the year. Syntheses of bioactive metabolites in leaf samples were significantly affected by sampling season as well as altitudinal locations respectively. Callus derived from leaf explants was maintained in Murashige and Skoog medium containing (0.25 mg l⁻¹) 2,4-Dichlorophenoxy-acetic acid (2,4-D) + (0.05 mg l⁻¹) Kinetin and (0.25 mg l⁻¹) α-Naphthaleneacetic acid + (0.5 mg l⁻¹) 6-Benzylaminopurine in liquid phase showed optimal conditions for accumulating biomass and synthesis of identified bioactive metabolites. |
| doi_str_mv | 10.1007/s10725-014-9956-2 |
| format | Article |
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Plant samples were collected during December–March, April–July and August–November in the year 2010–2012 from three different altitudinal locations (Nainital, Almora and Bageshwar districts) of Kumaon region, Uttarakhand, India. The results obtained from Q-TOF LC/MS analysis demonstrated the presence of four bioactive metabolites known for osteogenic activity in bark and stem samples round the year. Syntheses of bioactive metabolites in leaf samples were significantly affected by sampling season as well as altitudinal locations respectively. Callus derived from leaf explants was maintained in Murashige and Skoog medium containing (0.25 mg l⁻¹) 2,4-Dichlorophenoxy-acetic acid (2,4-D) + (0.05 mg l⁻¹) Kinetin and (0.25 mg l⁻¹) α-Naphthaleneacetic acid + (0.5 mg l⁻¹) 6-Benzylaminopurine in liquid phase showed optimal conditions for accumulating biomass and synthesis of identified bioactive metabolites.</description><identifier>ISSN: 0167-6903</identifier><identifier>EISSN: 1573-5087</identifier><identifier>DOI: 10.1007/s10725-014-9956-2</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>2,4-D ; Acetic acid ; Agriculture ; Bark ; benzyladenine ; biomass production ; Biomedical and Life Sciences ; callus ; callus culture ; Endangered plants ; explants ; kinetin ; Leaves ; Life Sciences ; Liquid chromatography ; Mass spectroscopy ; Medicinal plants ; medicinal properties ; Metabolites ; naphthaleneacetic acid ; Original Paper ; Plant Anatomy/Development ; Plant Physiology ; Plant Sciences ; Secondary metabolites ; spectroscopy ; Ulmus wallichiana</subject><ispartof>Plant growth regulation, 2015-01, Vol.75 (1), p.331-340</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><rights>Springer Science+Business Media Dordrecht 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-be3fe1e1bf8b2d34bd26ce85568f0a4d64c898dcacde46c67dc485112f3b8f8e3</citedby><cites>FETCH-LOGICAL-c340t-be3fe1e1bf8b2d34bd26ce85568f0a4d64c898dcacde46c67dc485112f3b8f8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10725-014-9956-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10725-014-9956-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Kumari, Tejaswita</creatorcontrib><creatorcontrib>Sharma, Chetan</creatorcontrib><creatorcontrib>Bajpai, Vikas</creatorcontrib><creatorcontrib>Kumar, Brijesh</creatorcontrib><creatorcontrib>Srivastava, Mukesh</creatorcontrib><creatorcontrib>Arya, K. R</creatorcontrib><title>Qualitative determination of bioactive metabolites through Q-TOF LC/MS in different parts and undifferentiated cultures of Ulmus wallichiana Planchon</title><title>Plant growth regulation</title><addtitle>Plant Growth Regul</addtitle><description>Ulmus wallichiana is an endangered and folk medicinal plant used for healing fractures in India. Recently, the secondary metabolites isolated and identified from its bark revealed osteogenic activity. Considering the medicinal properties of its bark and the source for its identified and isolated metabolites, the requirement for production of alternative pathways was crucial. This study aimed to determine its bioactive metabolite using Quadrupole time-of-flight liquid chromatography-mass spectroscopy (Q-TOF LC/MS) in different parts of this plant with seasonal and altitudinal variability pertaining with the development of a protocol for induction of callus cultures and biomass production. Plant samples were collected during December–March, April–July and August–November in the year 2010–2012 from three different altitudinal locations (Nainital, Almora and Bageshwar districts) of Kumaon region, Uttarakhand, India. The results obtained from Q-TOF LC/MS analysis demonstrated the presence of four bioactive metabolites known for osteogenic activity in bark and stem samples round the year. Syntheses of bioactive metabolites in leaf samples were significantly affected by sampling season as well as altitudinal locations respectively. Callus derived from leaf explants was maintained in Murashige and Skoog medium containing (0.25 mg l⁻¹) 2,4-Dichlorophenoxy-acetic acid (2,4-D) + (0.05 mg l⁻¹) Kinetin and (0.25 mg l⁻¹) α-Naphthaleneacetic acid + (0.5 mg l⁻¹) 6-Benzylaminopurine in liquid phase showed optimal conditions for accumulating biomass and synthesis of identified bioactive metabolites.</description><subject>2,4-D</subject><subject>Acetic acid</subject><subject>Agriculture</subject><subject>Bark</subject><subject>benzyladenine</subject><subject>biomass production</subject><subject>Biomedical and Life Sciences</subject><subject>callus</subject><subject>callus culture</subject><subject>Endangered plants</subject><subject>explants</subject><subject>kinetin</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Mass spectroscopy</subject><subject>Medicinal plants</subject><subject>medicinal properties</subject><subject>Metabolites</subject><subject>naphthaleneacetic acid</subject><subject>Original Paper</subject><subject>Plant Anatomy/Development</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Secondary metabolites</subject><subject>spectroscopy</subject><subject>Ulmus wallichiana</subject><issn>0167-6903</issn><issn>1573-5087</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kMtuFDEQRS0EEkPgA1hhibWJ3-1eohEBpEEhSmZtuf2YcdRjD7Y7iA_hf3FohFixKlXVvadUF4DXBL8jGA-XleCBCoQJR-MoJKJPwIaIgSGB1fAUbDCRA5IjZs_Bi1rvMcZKCbIBP28WM8dmWnzw0Pnmyymm3uUEc4BTzMb-Xp18M1PuSl9hO5a8HI7wBt1dX8Hd9vLLLYwJuhiCLz41eDalVWiSg0v6O42meQftMreldEin7-fTUuF3M8_RHqNJBn6dTbLHnF6CZ8HM1b_6Uy_A_urD3fYT2l1__Lx9v0OWcdzQ5FnwxJMpqIk6xidHpfVKCKkCNtxJbtWonDXWeS6tHJzl_WtCA5tUUJ5dgLcr91zyt8XXpu_zUlI_qYnklHBMRtZVZFXZkmstPuhziSdTfmiC9WP6ek1f9_T1Y_qadg9dPbVr08GXf8j_Mb1ZTcFkbQ4lVr2_pZgIjImSQhD2C__VlIc</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Kumari, Tejaswita</creator><creator>Sharma, Chetan</creator><creator>Bajpai, Vikas</creator><creator>Kumar, Brijesh</creator><creator>Srivastava, Mukesh</creator><creator>Arya, K. 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This study aimed to determine its bioactive metabolite using Quadrupole time-of-flight liquid chromatography-mass spectroscopy (Q-TOF LC/MS) in different parts of this plant with seasonal and altitudinal variability pertaining with the development of a protocol for induction of callus cultures and biomass production. Plant samples were collected during December–March, April–July and August–November in the year 2010–2012 from three different altitudinal locations (Nainital, Almora and Bageshwar districts) of Kumaon region, Uttarakhand, India. The results obtained from Q-TOF LC/MS analysis demonstrated the presence of four bioactive metabolites known for osteogenic activity in bark and stem samples round the year. Syntheses of bioactive metabolites in leaf samples were significantly affected by sampling season as well as altitudinal locations respectively. Callus derived from leaf explants was maintained in Murashige and Skoog medium containing (0.25 mg l⁻¹) 2,4-Dichlorophenoxy-acetic acid (2,4-D) + (0.05 mg l⁻¹) Kinetin and (0.25 mg l⁻¹) α-Naphthaleneacetic acid + (0.5 mg l⁻¹) 6-Benzylaminopurine in liquid phase showed optimal conditions for accumulating biomass and synthesis of identified bioactive metabolites.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><doi>10.1007/s10725-014-9956-2</doi><tpages>10</tpages></addata></record> |
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| subjects | 2,4-D Acetic acid Agriculture Bark benzyladenine biomass production Biomedical and Life Sciences callus callus culture Endangered plants explants kinetin Leaves Life Sciences Liquid chromatography Mass spectroscopy Medicinal plants medicinal properties Metabolites naphthaleneacetic acid Original Paper Plant Anatomy/Development Plant Physiology Plant Sciences Secondary metabolites spectroscopy Ulmus wallichiana |
| title | Qualitative determination of bioactive metabolites through Q-TOF LC/MS in different parts and undifferentiated cultures of Ulmus wallichiana Planchon |
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