Rational design and immunogenicity of liposome-based diepitope constructs: Application to synthetic oligosaccharides mimicking theShigella flexneri2a O-antigen
We have designed chemically defined diepitope constructs consisting of liposomes displaying at their surface synthetic oligosaccharides mimicking the O-antigen of theShigella flexneri2a lipopolysaccharide (B-cell epitope) and influenza hemagglutinin peptide HA 307-319 (Th epitope). Using well contro...
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| Veröffentlicht in: | Vaccine 2009-08, Vol.27 (39), p.5419 |
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| container_issue | 39 |
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| container_title | Vaccine |
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| creator | Said Hassane, Fatouma Phalipon, Armelle Tanguy, Myriam Guerreiro, Catherine Bélot, Frédéric Frisch, Benoît Mulard, Laurence A Schuber, Francis |
| description | We have designed chemically defined diepitope constructs consisting of liposomes displaying at their surface synthetic oligosaccharides mimicking the O-antigen of theShigella flexneri2a lipopolysaccharide (B-cell epitope) and influenza hemagglutinin peptide HA 307-319 (Th epitope). Using well controlled and high-yielding covalent bioconjugation reactions, the two structurally independent epitopes were coupled to the lipopeptide Pam3CAG, i.e. a TLR2 ligand known for its adjuvant properties, anchored in preformed vesicles. The synthetic construct containing a pentadecasaccharide corresponding to three O-antigen repeating units triggered T-dependent anti-oligosaccharide and anti-S. flexneri2a LPS antibody responses when administered i.m. to BALB/c mice. Moreover, the long-lasting anti-LPS antibody response afforded protection against aS. flexneri2a challenge. These results show that liposome diepitope constructs could be attractive alternatives in the development of synthetic carbohydrate-based vaccines. |
| doi_str_mv | 10.1016/j.vaccine.2009.06.031 |
| format | Article |
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Using well controlled and high-yielding covalent bioconjugation reactions, the two structurally independent epitopes were coupled to the lipopeptide Pam3CAG, i.e. a TLR2 ligand known for its adjuvant properties, anchored in preformed vesicles. The synthetic construct containing a pentadecasaccharide corresponding to three O-antigen repeating units triggered T-dependent anti-oligosaccharide and anti-S. flexneri2a LPS antibody responses when administered i.m. to BALB/c mice. Moreover, the long-lasting anti-LPS antibody response afforded protection against aS. flexneri2a challenge. 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Using well controlled and high-yielding covalent bioconjugation reactions, the two structurally independent epitopes were coupled to the lipopeptide Pam3CAG, i.e. a TLR2 ligand known for its adjuvant properties, anchored in preformed vesicles. The synthetic construct containing a pentadecasaccharide corresponding to three O-antigen repeating units triggered T-dependent anti-oligosaccharide and anti-S. flexneri2a LPS antibody responses when administered i.m. to BALB/c mice. Moreover, the long-lasting anti-LPS antibody response afforded protection against aS. flexneri2a challenge. These results show that liposome diepitope constructs could be attractive alternatives in the development of synthetic carbohydrate-based vaccines.</description><subject>Antigens</subject><subject>Carbohydrates</subject><subject>Design</subject><subject>Immune system</subject><subject>Immunogenicity</subject><subject>Lymphocytes</subject><subject>Peptides</subject><subject>Proteins</subject><subject>Studies</subject><subject>Vaccines</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNjs1KxDAUhYMoOP48gnDBdWuSznRadyKKO0FduBtietvemt7EJhXnaXxVi_gArg4cPs75hLhQMldSlVdD_mmsJcZcS1nnssxloQ7ESlXbItMbVR2KldTlOlsr-XosTmIcpJSbQtUr8f1kEnk2DhqM1DEYboDGcWbfIZOltAffgqPgox8xezMRG2gIAyUfEKznmKbZpngNNyE4sr97kDzEPaceE1nwjjofF8feTLT8wEgj2XfiDhbiuacOnTPQOvxinEgbeMwMp6XmM3HUGhfx_C9PxeX93cvtQxYm_zFjTLvBz9PiH3eqVFUttVbb4n_UDzA-Zt8</recordid><startdate>20090827</startdate><enddate>20090827</enddate><creator>Said 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Using well controlled and high-yielding covalent bioconjugation reactions, the two structurally independent epitopes were coupled to the lipopeptide Pam3CAG, i.e. a TLR2 ligand known for its adjuvant properties, anchored in preformed vesicles. The synthetic construct containing a pentadecasaccharide corresponding to three O-antigen repeating units triggered T-dependent anti-oligosaccharide and anti-S. flexneri2a LPS antibody responses when administered i.m. to BALB/c mice. Moreover, the long-lasting anti-LPS antibody response afforded protection against aS. flexneri2a challenge. These results show that liposome diepitope constructs could be attractive alternatives in the development of synthetic carbohydrate-based vaccines.</abstract><cop>Kidlington</cop><pub>Elsevier Limited</pub><doi>10.1016/j.vaccine.2009.06.031</doi></addata></record> |
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| source | ScienceDirect Journals (5 years ago - present); ProQuest Central UK/Ireland |
| subjects | Antigens Carbohydrates Design Immune system Immunogenicity Lymphocytes Peptides Proteins Studies Vaccines |
| title | Rational design and immunogenicity of liposome-based diepitope constructs: Application to synthetic oligosaccharides mimicking theShigella flexneri2a O-antigen |
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