Osmotic stress decreases PIP aquaporin transcripts in barley roots but H₂O₂ is not involved in this process
Previous reports indicate that salt stress reduces the root hydraulic conductance and the expression of plasmamembrane-type aquaporins (PIPs). As a molecular mechanism for this phenomenon, the present study found evidence that the osmotic component, but probably not an ion-specific component, decrea...
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| Veröffentlicht in: | Journal of plant research 2014-11, Vol.127 (6), p.787-792 |
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| creator | Katsuhara, Maki Tsuji, Nobuya Shibasaka, Mineo Panda, Sanjib Kumar |
| description | Previous reports indicate that salt stress reduces the root hydraulic conductance and the expression of plasmamembrane-type aquaporins (PIPs). As a molecular mechanism for this phenomenon, the present study found evidence that the osmotic component, but probably not an ion-specific component, decreases PIP transcripts. Eight of ten PIP transcripts were reduced to less than half by 360 mM mannitol treatment for 12 h in comparison with control samples. A large decrease of HvPIP2;1 protein was also recorded. This reduction of both transcripts and proteins of HvPIP2s should be physiologically effective for preventing or reducing dehydration at an initial phase of severe salt/osmotic stress. Root cell sap osmolality increased from 278 to 372 mOsm 24 h after 360 mM mannitol treatment. These steps can secure survival and growth recovery with water reabsorption in barley. Our data also suggest that H₂O₂ seems not to be the main cause of osmotic stress-induced transcriptional down-regulation within the concentrations (20–500 μM) and time periods (24 h) examined, although H₂O₂ was previously proposed to be involved in the mechanisms of salinity/osmotic tolerance. |
| doi_str_mv | 10.1007/s10265-014-0662-y |
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As a molecular mechanism for this phenomenon, the present study found evidence that the osmotic component, but probably not an ion-specific component, decreases PIP transcripts. Eight of ten PIP transcripts were reduced to less than half by 360 mM mannitol treatment for 12 h in comparison with control samples. A large decrease of HvPIP2;1 protein was also recorded. This reduction of both transcripts and proteins of HvPIP2s should be physiologically effective for preventing or reducing dehydration at an initial phase of severe salt/osmotic stress. Root cell sap osmolality increased from 278 to 372 mOsm 24 h after 360 mM mannitol treatment. These steps can secure survival and growth recovery with water reabsorption in barley. Our data also suggest that H₂O₂ seems not to be the main cause of osmotic stress-induced transcriptional down-regulation within the concentrations (20–500 μM) and time periods (24 h) examined, although H₂O₂ was previously proposed to be involved in the mechanisms of salinity/osmotic tolerance.</description><identifier>ISSN: 0918-9440</identifier><identifier>EISSN: 1618-0860</identifier><identifier>DOI: 10.1007/s10265-014-0662-y</identifier><identifier>PMID: 25193635</identifier><language>eng</language><publisher>Tokyo: Springer-Verlag</publisher><subject>Abiotic stress ; aquaporins ; Aquaporins - genetics ; Aquaporins - metabolism ; Barley ; Biomedical and Life Sciences ; Dehydration ; Gene Expression Regulation, Plant - drug effects ; Hordeum - drug effects ; Hordeum - genetics ; Hordeum - metabolism ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Life Sciences ; mannitol ; osmolality ; Osmosis ; Osmotic Pressure ; osmotic stress ; osmotolerance ; Plant Biochemistry ; Plant Ecology ; Plant Physiology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots - drug effects ; Plant Roots - metabolism ; Plant Sciences ; Plant tolerance ; Regular Paper ; root hydraulic conductivity ; roots ; Salinity ; salt stress ; sap ; Sodium Chloride - metabolism ; Stress, Physiological - drug effects ; transcription (genetics) ; Transcription factors ; Water - metabolism</subject><ispartof>Journal of plant research, 2014-11, Vol.127 (6), p.787-792</ispartof><rights>The Botanical Society of Japan and Springer Japan 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-b501fd85db8f5912224b307c6c778e760620ba506aaa231572d08439be5c28bc3</citedby><cites>FETCH-LOGICAL-c396t-b501fd85db8f5912224b307c6c778e760620ba506aaa231572d08439be5c28bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10265-014-0662-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10265-014-0662-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25193635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katsuhara, Maki</creatorcontrib><creatorcontrib>Tsuji, Nobuya</creatorcontrib><creatorcontrib>Shibasaka, Mineo</creatorcontrib><creatorcontrib>Panda, Sanjib Kumar</creatorcontrib><title>Osmotic stress decreases PIP aquaporin transcripts in barley roots but H₂O₂ is not involved in this process</title><title>Journal of plant research</title><addtitle>J Plant Res</addtitle><addtitle>J Plant Res</addtitle><description>Previous reports indicate that salt stress reduces the root hydraulic conductance and the expression of plasmamembrane-type aquaporins (PIPs). As a molecular mechanism for this phenomenon, the present study found evidence that the osmotic component, but probably not an ion-specific component, decreases PIP transcripts. Eight of ten PIP transcripts were reduced to less than half by 360 mM mannitol treatment for 12 h in comparison with control samples. A large decrease of HvPIP2;1 protein was also recorded. This reduction of both transcripts and proteins of HvPIP2s should be physiologically effective for preventing or reducing dehydration at an initial phase of severe salt/osmotic stress. Root cell sap osmolality increased from 278 to 372 mOsm 24 h after 360 mM mannitol treatment. These steps can secure survival and growth recovery with water reabsorption in barley. Our data also suggest that H₂O₂ seems not to be the main cause of osmotic stress-induced transcriptional down-regulation within the concentrations (20–500 μM) and time periods (24 h) examined, although H₂O₂ was previously proposed to be involved in the mechanisms of salinity/osmotic tolerance.</description><subject>Abiotic stress</subject><subject>aquaporins</subject><subject>Aquaporins - genetics</subject><subject>Aquaporins - metabolism</subject><subject>Barley</subject><subject>Biomedical and Life Sciences</subject><subject>Dehydration</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Hordeum - drug effects</subject><subject>Hordeum - genetics</subject><subject>Hordeum - metabolism</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Life Sciences</subject><subject>mannitol</subject><subject>osmolality</subject><subject>Osmosis</subject><subject>Osmotic Pressure</subject><subject>osmotic stress</subject><subject>osmotolerance</subject><subject>Plant Biochemistry</subject><subject>Plant Ecology</subject><subject>Plant Physiology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Roots - drug effects</subject><subject>Plant Roots - metabolism</subject><subject>Plant Sciences</subject><subject>Plant tolerance</subject><subject>Regular Paper</subject><subject>root hydraulic conductivity</subject><subject>roots</subject><subject>Salinity</subject><subject>salt stress</subject><subject>sap</subject><subject>Sodium Chloride - metabolism</subject><subject>Stress, Physiological - drug effects</subject><subject>transcription (genetics)</subject><subject>Transcription factors</subject><subject>Water - metabolism</subject><issn>0918-9440</issn><issn>1618-0860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kM1q3DAUhUVpaCZpH6CbVtC10yvJ-vGyhDYJBCaQZi0kWU4dZqyJrhyYbR61T1INTktWXQjpXn3nHDiEfGRwxgD0V2TAlWyAtQ0oxZv9G7JiipkGjIK3ZAVdfXdtC8fkBPEBgGnZmXfkmEvWCSXkiqQ1blMZA8WSIyLtY8jRYUR6c3VD3ePsdimPEy3ZTRjyuCtI6-hd3sQ9zSnV2c-FXv5-fl7XQ0ekUyqVeUqbp9gf4PKrLnc5hRrwnhwNboPxw8t9Su5-fP95ftlcry-uzr9dN0F0qjReAht6I3tvBtkxznnrBeiggtYmagWKg3cSlHOOCyY178G0ovNRBm58EKfky-Jbcx_niMU-pDlPNdIeGgKlDWeVYgsVckLMcbC7PG5d3lsG9lCxXSq2tWJ7qNjuq-bTi_Pst7H_p_jbaQX4AmD9mu5jfhX9H9fPi2hwybr7PKK9u-XAJABIJYUWfwCV15Ix</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Katsuhara, Maki</creator><creator>Tsuji, Nobuya</creator><creator>Shibasaka, Mineo</creator><creator>Panda, Sanjib Kumar</creator><general>Springer-Verlag</general><general>Springer Japan</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7ST</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope></search><sort><creationdate>20141101</creationdate><title>Osmotic stress decreases PIP aquaporin transcripts in barley roots but H₂O₂ is not involved in this process</title><author>Katsuhara, Maki ; Tsuji, Nobuya ; Shibasaka, Mineo ; Panda, Sanjib Kumar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-b501fd85db8f5912224b307c6c778e760620ba506aaa231572d08439be5c28bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Abiotic stress</topic><topic>aquaporins</topic><topic>Aquaporins - 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As a molecular mechanism for this phenomenon, the present study found evidence that the osmotic component, but probably not an ion-specific component, decreases PIP transcripts. Eight of ten PIP transcripts were reduced to less than half by 360 mM mannitol treatment for 12 h in comparison with control samples. A large decrease of HvPIP2;1 protein was also recorded. This reduction of both transcripts and proteins of HvPIP2s should be physiologically effective for preventing or reducing dehydration at an initial phase of severe salt/osmotic stress. Root cell sap osmolality increased from 278 to 372 mOsm 24 h after 360 mM mannitol treatment. These steps can secure survival and growth recovery with water reabsorption in barley. Our data also suggest that H₂O₂ seems not to be the main cause of osmotic stress-induced transcriptional down-regulation within the concentrations (20–500 μM) and time periods (24 h) examined, although H₂O₂ was previously proposed to be involved in the mechanisms of salinity/osmotic tolerance.</abstract><cop>Tokyo</cop><pub>Springer-Verlag</pub><pmid>25193635</pmid><doi>10.1007/s10265-014-0662-y</doi><tpages>6</tpages></addata></record> |
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| subjects | Abiotic stress aquaporins Aquaporins - genetics Aquaporins - metabolism Barley Biomedical and Life Sciences Dehydration Gene Expression Regulation, Plant - drug effects Hordeum - drug effects Hordeum - genetics Hordeum - metabolism Hydrogen peroxide Hydrogen Peroxide - metabolism Life Sciences mannitol osmolality Osmosis Osmotic Pressure osmotic stress osmotolerance Plant Biochemistry Plant Ecology Plant Physiology Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - drug effects Plant Roots - metabolism Plant Sciences Plant tolerance Regular Paper root hydraulic conductivity roots Salinity salt stress sap Sodium Chloride - metabolism Stress, Physiological - drug effects transcription (genetics) Transcription factors Water - metabolism |
| title | Osmotic stress decreases PIP aquaporin transcripts in barley roots but H₂O₂ is not involved in this process |
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