A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance
Anti-lipopolysaccharide factors (ALFs) are basic components of the crustacean immune system that defend against a range of pathogens. The cDNA sequence of a new ALF, designated nLvALF2, with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the cons...
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| Veröffentlicht in: | Chinese journal of oceanology and limnology 2014-11, Vol.32 (6), p.1232-1247 |
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| creator | Liu, Jingwen Yu, Yang Li, Fuhua Zhang, Xiaojun Xiang, Jianhai |
| description | Anti-lipopolysaccharide factors (ALFs) are basic components of the crustacean immune system that defend against a range of pathogens. The cDNA sequence of a new ALF, designated nLvALF2, with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the conserved functional domain of ALFs, the LPS binding domain (LBD). Its genomic sequence consisted of three exons and four introns. nLvALF2 was mainly expressed in the Oka organ and gills of shrimps. The transcriptional level of nLvALF2 increased significantly after white spot syndrome virus (WSSV) infection, suggesting its important roles in protecting shrimps from WSSV. Single nucleotide polymorphisms (SNPs) were found in the genomic sequence of nLvALF2, of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV. The loci g.2422 A>G, g.2466 T>C, and g.2529 G>A were significantly associated with the resistance to WSSV (P |
| doi_str_mv | 10.1007/s00343-015-4010-4 |
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The cDNA sequence of a new ALF, designated nLvALF2, with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the conserved functional domain of ALFs, the LPS binding domain (LBD). Its genomic sequence consisted of three exons and four introns. nLvALF2 was mainly expressed in the Oka organ and gills of shrimps. The transcriptional level of nLvALF2 increased significantly after white spot syndrome virus (WSSV) infection, suggesting its important roles in protecting shrimps from WSSV. Single nucleotide polymorphisms (SNPs) were found in the genomic sequence of nLvALF2, of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV. The loci g.2422 A>G, g.2466 T>C, and g.2529 G>A were significantly associated with the resistance to WSSV (P<0.05). These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei.</description><identifier>ISSN: 0254-4059</identifier><identifier>ISSN: 2096-5508</identifier><identifier>EISSN: 1993-5005</identifier><identifier>EISSN: 2523-3521</identifier><identifier>DOI: 10.1007/s00343-015-4010-4</identifier><language>eng</language><publisher>Heidelberg: Springer-Verlag</publisher><subject>ALF ; Amino acid sequence ; Amino acid sequences ; Amino acids ; Animal diseases ; Aquatic crustaceans ; Biology ; cDNA序列 ; Complementary DNA ; Conserved sequence ; Crustaceans ; Decapoda ; Earth and Environmental Science ; Earth Sciences ; Exons ; Genomics ; Gills ; Immune system ; Immunity ; Introns ; Lipopolysaccharides ; Litopenaeus vannamei ; loci ; Marine biology ; Nucleotides ; Oceanography ; open reading frames ; Pathogens ; Polymorphism ; Resistance factors ; Sequencing ; shrimp ; Shrimps ; Single-nucleotide polymorphism ; transcription (genetics) ; White spot syndrome ; White spot syndrome virus ; WSSV ; 凡纳滨对虾 ; 单核苷酸多态性 ; 基因组序列 ; 氨基酸序列 ; 白斑综合症病毒</subject><ispartof>Chinese journal of oceanology and limnology, 2014-11, Vol.32 (6), p.1232-1247</ispartof><rights>Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2014</rights><rights>Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2014.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-58a8bc4bda5782200e0da194fa5fadaf80762fd1417179cad4b81759dd18d6fa3</citedby><cites>FETCH-LOGICAL-c424t-58a8bc4bda5782200e0da194fa5fadaf80762fd1417179cad4b81759dd18d6fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84119X/84119X.jpg</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1618066999/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1618066999?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>315,781,785,21390,21391,21392,21393,23258,27926,27927,33532,33705,33746,34007,34316,43661,43789,43807,43955,44069,64387,64391,72471,74106,74285,74304,74475,74592</link.rule.ids></links><search><creatorcontrib>Liu, Jingwen</creatorcontrib><creatorcontrib>Yu, Yang</creatorcontrib><creatorcontrib>Li, Fuhua</creatorcontrib><creatorcontrib>Zhang, Xiaojun</creatorcontrib><creatorcontrib>Xiang, Jianhai</creatorcontrib><title>A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance</title><title>Chinese journal of oceanology and limnology</title><addtitle>Chin. J. Ocean. Limnol</addtitle><addtitle>Chinese Journal of Oceanology and Limnology</addtitle><description>Anti-lipopolysaccharide factors (ALFs) are basic components of the crustacean immune system that defend against a range of pathogens. The cDNA sequence of a new ALF, designated nLvALF2, with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the conserved functional domain of ALFs, the LPS binding domain (LBD). Its genomic sequence consisted of three exons and four introns. nLvALF2 was mainly expressed in the Oka organ and gills of shrimps. The transcriptional level of nLvALF2 increased significantly after white spot syndrome virus (WSSV) infection, suggesting its important roles in protecting shrimps from WSSV. Single nucleotide polymorphisms (SNPs) were found in the genomic sequence of nLvALF2, of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV. The loci g.2422 A>G, g.2466 T>C, and g.2529 G>A were significantly associated with the resistance to WSSV (P<0.05). These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei.</description><subject>ALF</subject><subject>Amino acid sequence</subject><subject>Amino acid sequences</subject><subject>Amino acids</subject><subject>Animal diseases</subject><subject>Aquatic crustaceans</subject><subject>Biology</subject><subject>cDNA序列</subject><subject>Complementary DNA</subject><subject>Conserved sequence</subject><subject>Crustaceans</subject><subject>Decapoda</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>Exons</subject><subject>Genomics</subject><subject>Gills</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Introns</subject><subject>Lipopolysaccharides</subject><subject>Litopenaeus vannamei</subject><subject>loci</subject><subject>Marine biology</subject><subject>Nucleotides</subject><subject>Oceanography</subject><subject>open reading frames</subject><subject>Pathogens</subject><subject>Polymorphism</subject><subject>Resistance factors</subject><subject>Sequencing</subject><subject>shrimp</subject><subject>Shrimps</subject><subject>Single-nucleotide polymorphism</subject><subject>transcription (genetics)</subject><subject>White spot syndrome</subject><subject>White spot syndrome virus</subject><subject>WSSV</subject><subject>凡纳滨对虾</subject><subject>单核苷酸多态性</subject><subject>基因组序列</subject><subject>氨基酸序列</subject><subject>白斑综合症病毒</subject><issn>0254-4059</issn><issn>2096-5508</issn><issn>1993-5005</issn><issn>2523-3521</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kEFvEzEQhS0EEiHwAzhhwYXLwozX3rWPUdUCIgKkUDhak7Udtkrs1N6A-Pc42gohDj1ZI3_vzZvH2HOENwjQvy0ArWwbQNVIQGjkA7ZAY9pGAaiHbAFCyfqjzGP2pJSbShsJZsE-rnj0v_hqfcVDTge-Hqd09JH8qfCfFCMd_MgpOj5OhW8-fSk8-z1N3vEp8e-bzbc6l7FMFAf_lD0KtC_-2d27ZNdXl18v3jfrz-8-XKzWzSCFnBqlSW8HuXWkei0EgAdHaGQgFchR0NB3IjiU2GNvBnJyq7FXxjnUrgvULtnr2feY0-3Jl8kexjL4_Z6iT6disRO90QqlrOir_9CbdMqxpqsUaug6U0taMpypIadSsg_2mMcD5d8WwZ7rtXO9ttZrz_Xas7OYNaWycefzP873iF7MokDJ0i6PxV5vRAWg8rJFfS-B9SJRiZd3YX-kuLutu_-m7Tphut6orv0DPz2YbQ</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Liu, 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SNPs related to WSSV resistance</title><author>Liu, Jingwen ; Yu, Yang ; Li, Fuhua ; Zhang, Xiaojun ; Xiang, Jianhai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-58a8bc4bda5782200e0da194fa5fadaf80762fd1417179cad4b81759dd18d6fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>ALF</topic><topic>Amino acid sequence</topic><topic>Amino acid sequences</topic><topic>Amino acids</topic><topic>Animal diseases</topic><topic>Aquatic crustaceans</topic><topic>Biology</topic><topic>cDNA序列</topic><topic>Complementary DNA</topic><topic>Conserved sequence</topic><topic>Crustaceans</topic><topic>Decapoda</topic><topic>Earth and Environmental Science</topic><topic>Earth Sciences</topic><topic>Exons</topic><topic>Genomics</topic><topic>Gills</topic><topic>Immune system</topic><topic>Immunity</topic><topic>Introns</topic><topic>Lipopolysaccharides</topic><topic>Litopenaeus 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Fuhua</au><au>Zhang, Xiaojun</au><au>Xiang, Jianhai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance</atitle><jtitle>Chinese journal of oceanology and limnology</jtitle><stitle>Chin. J. Ocean. Limnol</stitle><addtitle>Chinese Journal of Oceanology and Limnology</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>32</volume><issue>6</issue><spage>1232</spage><epage>1247</epage><pages>1232-1247</pages><issn>0254-4059</issn><issn>2096-5508</issn><eissn>1993-5005</eissn><eissn>2523-3521</eissn><abstract>Anti-lipopolysaccharide factors (ALFs) are basic components of the crustacean immune system that defend against a range of pathogens. The cDNA sequence of a new ALF, designated nLvALF2, with an open reading frame encoding 132 amino acids was cloned. Its deduced amino acid sequence contained the conserved functional domain of ALFs, the LPS binding domain (LBD). Its genomic sequence consisted of three exons and four introns. nLvALF2 was mainly expressed in the Oka organ and gills of shrimps. The transcriptional level of nLvALF2 increased significantly after white spot syndrome virus (WSSV) infection, suggesting its important roles in protecting shrimps from WSSV. Single nucleotide polymorphisms (SNPs) were found in the genomic sequence of nLvALF2, of which 38 were analyzed for associations with the susceptibility/resistance of shrimps to WSSV. The loci g.2422 A>G, g.2466 T>C, and g.2529 G>A were significantly associated with the resistance to WSSV (P<0.05). These SNP loci could be developed as markers for selection of WSSV-resistant varieties of Litopenaeus vannamei.</abstract><cop>Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s00343-015-4010-4</doi><tpages>16</tpages></addata></record> |
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| subjects | ALF Amino acid sequence Amino acid sequences Amino acids Animal diseases Aquatic crustaceans Biology cDNA序列 Complementary DNA Conserved sequence Crustaceans Decapoda Earth and Environmental Science Earth Sciences Exons Genomics Gills Immune system Immunity Introns Lipopolysaccharides Litopenaeus vannamei loci Marine biology Nucleotides Oceanography open reading frames Pathogens Polymorphism Resistance factors Sequencing shrimp Shrimps Single-nucleotide polymorphism transcription (genetics) White spot syndrome White spot syndrome virus WSSV 凡纳滨对虾 单核苷酸多态性 基因组序列 氨基酸序列 白斑综合症病毒 |
| title | A new ALF from Litopenaeus vannamei and its SNPs related to WSSV resistance |
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