sup 99m^Tc-cAbVCAM1-5 Imaging Is a Sensitive and Reproducible Tool for the Detection of Inflamed Atherosclerotic Lesions in Mice
...Tc-cAbVCAM1-5, a single-domain antibody fragment directed against mouse or human vascular cell adhesion molecule 1 (VCAM-1), recently has been proposed as a new imaging agent for the detection of inflamed atherosclerotic lesions. Indeed, in a mouse model of atherosclerosis, ...Tc-cAbVCAM1-5 speci...
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| Veröffentlicht in: | The Journal of nuclear medicine (1978) 2014-10, Vol.55 (10), p.1678 |
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| container_title | The Journal of nuclear medicine (1978) |
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| creator | Broisat, Alexis Toczek, Jakub Dumas, Laurent S Ahmadi, Mitra Bacot, Sandrine Perret, Pascale Slimani, Lotfi Barone-Rochette, Gilles Soubies, Audrey Devoogdt, Nick Lahoutte, Tony Fagret, Daniel Riou, Laurent M Ghezzi, Catherine |
| description | ...Tc-cAbVCAM1-5, a single-domain antibody fragment directed against mouse or human vascular cell adhesion molecule 1 (VCAM-1), recently has been proposed as a new imaging agent for the detection of inflamed atherosclerotic lesions. Indeed, in a mouse model of atherosclerosis, ...Tc-cAbVCAM1-5 specifically bound to VCAM-1-positive lesions, thereby allowing their identification on SPECT images. The purpose of the present study was to investigate ...Tc-cAbVCAM1-5 imaging sensitivity using a reference statin therapy. Thirty apolipoprotein E-deficient mice were fed a western-type diet. First, the relationship between the level of VCAM-1 expression and ...Tc-cAbVCAM1-5 uptake was evaluated in 18 mice using immunohistochemistry and autoradiography. Second, longitudinal SPECT/CT imaging was performed on control (n = 9) or atorvastatin-treated mice (0.01% w/w, n = 9). ...Tc-cAbVCAM1-5 uptake in atherosclerotic lesions correlated with the level of VCAM-1 expression (P < 0.05). Atorvastatin exerted significant antiatherogenic effects, and ...Tc-cAbVCAM1-5 lesion uptake was significantly reduced in 35-wk-old atorvastatin-treated mice, as indicated by ex vivo ...-well counting and autoradiography (P < 0.05). SPECT imaging quantification based on contrast-enhanced CT was reproducible (interexperimenter intraclass correlation coefficient, 0.97; intraexperimenter intraclass correlation coefficient, 0.90), and yielded results that were highly correlated with tracer biodistribution (r = 0.83; P < 0.0001). Therefore, reduced ...Tc-cAbVCAM1-5 uptake in atorvastatin-treated mice was successfully monitored noninvasively by SPECT/CT imaging (0.87 ± 0.06 vs. 1.11 ± 0.09 percentage injected dose per cubic centimeter in control group, P < 0.05). ...Tc-cAbVCAM1-5 imaging allowed the specific, sensitive, and reproducible quantification of VCAM-1 expression in mouse atherosclerotic lesions. ...Tc-cAbVCAM1-5 therefore exhibits suitable characteristics for the evaluation of novel antiatherogenic agents. (ProQuest: ... denotes formulae/symbols omitted.) |
| format | Article |
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Indeed, in a mouse model of atherosclerosis, ...Tc-cAbVCAM1-5 specifically bound to VCAM-1-positive lesions, thereby allowing their identification on SPECT images. The purpose of the present study was to investigate ...Tc-cAbVCAM1-5 imaging sensitivity using a reference statin therapy. Thirty apolipoprotein E-deficient mice were fed a western-type diet. First, the relationship between the level of VCAM-1 expression and ...Tc-cAbVCAM1-5 uptake was evaluated in 18 mice using immunohistochemistry and autoradiography. Second, longitudinal SPECT/CT imaging was performed on control (n = 9) or atorvastatin-treated mice (0.01% w/w, n = 9). ...Tc-cAbVCAM1-5 uptake in atherosclerotic lesions correlated with the level of VCAM-1 expression (P < 0.05). Atorvastatin exerted significant antiatherogenic effects, and ...Tc-cAbVCAM1-5 lesion uptake was significantly reduced in 35-wk-old atorvastatin-treated mice, as indicated by ex vivo ...-well counting and autoradiography (P < 0.05). SPECT imaging quantification based on contrast-enhanced CT was reproducible (interexperimenter intraclass correlation coefficient, 0.97; intraexperimenter intraclass correlation coefficient, 0.90), and yielded results that were highly correlated with tracer biodistribution (r = 0.83; P < 0.0001). Therefore, reduced ...Tc-cAbVCAM1-5 uptake in atorvastatin-treated mice was successfully monitored noninvasively by SPECT/CT imaging (0.87 ± 0.06 vs. 1.11 ± 0.09 percentage injected dose per cubic centimeter in control group, P < 0.05). ...Tc-cAbVCAM1-5 imaging allowed the specific, sensitive, and reproducible quantification of VCAM-1 expression in mouse atherosclerotic lesions. ...Tc-cAbVCAM1-5 therefore exhibits suitable characteristics for the evaluation of novel antiatherogenic agents. 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Indeed, in a mouse model of atherosclerosis, ...Tc-cAbVCAM1-5 specifically bound to VCAM-1-positive lesions, thereby allowing their identification on SPECT images. The purpose of the present study was to investigate ...Tc-cAbVCAM1-5 imaging sensitivity using a reference statin therapy. Thirty apolipoprotein E-deficient mice were fed a western-type diet. First, the relationship between the level of VCAM-1 expression and ...Tc-cAbVCAM1-5 uptake was evaluated in 18 mice using immunohistochemistry and autoradiography. Second, longitudinal SPECT/CT imaging was performed on control (n = 9) or atorvastatin-treated mice (0.01% w/w, n = 9). ...Tc-cAbVCAM1-5 uptake in atherosclerotic lesions correlated with the level of VCAM-1 expression (P < 0.05). Atorvastatin exerted significant antiatherogenic effects, and ...Tc-cAbVCAM1-5 lesion uptake was significantly reduced in 35-wk-old atorvastatin-treated mice, as indicated by ex vivo ...-well counting and autoradiography (P < 0.05). SPECT imaging quantification based on contrast-enhanced CT was reproducible (interexperimenter intraclass correlation coefficient, 0.97; intraexperimenter intraclass correlation coefficient, 0.90), and yielded results that were highly correlated with tracer biodistribution (r = 0.83; P < 0.0001). Therefore, reduced ...Tc-cAbVCAM1-5 uptake in atorvastatin-treated mice was successfully monitored noninvasively by SPECT/CT imaging (0.87 ± 0.06 vs. 1.11 ± 0.09 percentage injected dose per cubic centimeter in control group, P < 0.05). ...Tc-cAbVCAM1-5 imaging allowed the specific, sensitive, and reproducible quantification of VCAM-1 expression in mouse atherosclerotic lesions. ...Tc-cAbVCAM1-5 therefore exhibits suitable characteristics for the evaluation of novel antiatherogenic agents. (ProQuest: ... denotes formulae/symbols omitted.)</description><subject>Apolipoproteins</subject><subject>Atherosclerosis</subject><subject>Immunohistochemistry</subject><subject>Medical imaging</subject><subject>Nuclear medicine</subject><subject>Rodents</subject><subject>Statins</subject><issn>0161-5505</issn><issn>1535-5667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNj7FuwjAURS1EJQLlH57UOZKj1MEZI0rVSLC0UUeQcV7AyLEhz-ncT8cDH8By73COdHUnLMlELlJRFKspS3hWZKkQXMzYnOjCOS-klAn7p_EKZdnvG53q6vi7rnbRg7pXJ-NOUBMo-EFHJpg_BOVa-Mbr4NtRm6NFaLy30PkBwhnhAwPqYLwD30HtOqt6bKGKaPCkbcxgNGyRokJgHOyMxlf20ilLuHz0gr19bpr1VxpXbiNSOFz8OLiIDvFC_l6uhJT5c9YdqzdPzg</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Broisat, Alexis</creator><creator>Toczek, Jakub</creator><creator>Dumas, Laurent S</creator><creator>Ahmadi, Mitra</creator><creator>Bacot, Sandrine</creator><creator>Perret, Pascale</creator><creator>Slimani, Lotfi</creator><creator>Barone-Rochette, Gilles</creator><creator>Soubies, Audrey</creator><creator>Devoogdt, Nick</creator><creator>Lahoutte, Tony</creator><creator>Fagret, Daniel</creator><creator>Riou, Laurent M</creator><creator>Ghezzi, Catherine</creator><general>Society of Nuclear Medicine</general><scope>4T-</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>NAPCQ</scope><scope>P64</scope></search><sort><creationdate>20141001</creationdate><title>sup 99m^Tc-cAbVCAM1-5 Imaging Is a Sensitive and Reproducible Tool for the Detection of Inflamed Atherosclerotic Lesions in Mice</title><author>Broisat, Alexis ; Toczek, Jakub ; Dumas, Laurent S ; Ahmadi, Mitra ; Bacot, Sandrine ; Perret, Pascale ; Slimani, Lotfi ; Barone-Rochette, Gilles ; Soubies, Audrey ; Devoogdt, Nick ; Lahoutte, Tony ; Fagret, Daniel ; Riou, Laurent M ; Ghezzi, Catherine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_16134975883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Apolipoproteins</topic><topic>Atherosclerosis</topic><topic>Immunohistochemistry</topic><topic>Medical imaging</topic><topic>Nuclear medicine</topic><topic>Rodents</topic><topic>Statins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Broisat, Alexis</creatorcontrib><creatorcontrib>Toczek, Jakub</creatorcontrib><creatorcontrib>Dumas, Laurent S</creatorcontrib><creatorcontrib>Ahmadi, Mitra</creatorcontrib><creatorcontrib>Bacot, Sandrine</creatorcontrib><creatorcontrib>Perret, Pascale</creatorcontrib><creatorcontrib>Slimani, Lotfi</creatorcontrib><creatorcontrib>Barone-Rochette, Gilles</creatorcontrib><creatorcontrib>Soubies, Audrey</creatorcontrib><creatorcontrib>Devoogdt, Nick</creatorcontrib><creatorcontrib>Lahoutte, Tony</creatorcontrib><creatorcontrib>Fagret, Daniel</creatorcontrib><creatorcontrib>Riou, Laurent M</creatorcontrib><creatorcontrib>Ghezzi, Catherine</creatorcontrib><collection>Docstoc</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>The Journal of nuclear medicine (1978)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Broisat, Alexis</au><au>Toczek, Jakub</au><au>Dumas, Laurent S</au><au>Ahmadi, Mitra</au><au>Bacot, Sandrine</au><au>Perret, Pascale</au><au>Slimani, Lotfi</au><au>Barone-Rochette, Gilles</au><au>Soubies, Audrey</au><au>Devoogdt, Nick</au><au>Lahoutte, Tony</au><au>Fagret, Daniel</au><au>Riou, Laurent M</au><au>Ghezzi, Catherine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>sup 99m^Tc-cAbVCAM1-5 Imaging Is a Sensitive and Reproducible Tool for the Detection of Inflamed Atherosclerotic Lesions in Mice</atitle><jtitle>The Journal of nuclear medicine (1978)</jtitle><date>2014-10-01</date><risdate>2014</risdate><volume>55</volume><issue>10</issue><spage>1678</spage><pages>1678-</pages><issn>0161-5505</issn><eissn>1535-5667</eissn><coden>JNMEAQ</coden><abstract>...Tc-cAbVCAM1-5, a single-domain antibody fragment directed against mouse or human vascular cell adhesion molecule 1 (VCAM-1), recently has been proposed as a new imaging agent for the detection of inflamed atherosclerotic lesions. Indeed, in a mouse model of atherosclerosis, ...Tc-cAbVCAM1-5 specifically bound to VCAM-1-positive lesions, thereby allowing their identification on SPECT images. The purpose of the present study was to investigate ...Tc-cAbVCAM1-5 imaging sensitivity using a reference statin therapy. Thirty apolipoprotein E-deficient mice were fed a western-type diet. First, the relationship between the level of VCAM-1 expression and ...Tc-cAbVCAM1-5 uptake was evaluated in 18 mice using immunohistochemistry and autoradiography. Second, longitudinal SPECT/CT imaging was performed on control (n = 9) or atorvastatin-treated mice (0.01% w/w, n = 9). ...Tc-cAbVCAM1-5 uptake in atherosclerotic lesions correlated with the level of VCAM-1 expression (P < 0.05). Atorvastatin exerted significant antiatherogenic effects, and ...Tc-cAbVCAM1-5 lesion uptake was significantly reduced in 35-wk-old atorvastatin-treated mice, as indicated by ex vivo ...-well counting and autoradiography (P < 0.05). SPECT imaging quantification based on contrast-enhanced CT was reproducible (interexperimenter intraclass correlation coefficient, 0.97; intraexperimenter intraclass correlation coefficient, 0.90), and yielded results that were highly correlated with tracer biodistribution (r = 0.83; P < 0.0001). Therefore, reduced ...Tc-cAbVCAM1-5 uptake in atorvastatin-treated mice was successfully monitored noninvasively by SPECT/CT imaging (0.87 ± 0.06 vs. 1.11 ± 0.09 percentage injected dose per cubic centimeter in control group, P < 0.05). ...Tc-cAbVCAM1-5 imaging allowed the specific, sensitive, and reproducible quantification of VCAM-1 expression in mouse atherosclerotic lesions. ...Tc-cAbVCAM1-5 therefore exhibits suitable characteristics for the evaluation of novel antiatherogenic agents. (ProQuest: ... denotes formulae/symbols omitted.)</abstract><cop>New York</cop><pub>Society of Nuclear Medicine</pub></addata></record> |
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| subjects | Apolipoproteins Atherosclerosis Immunohistochemistry Medical imaging Nuclear medicine Rodents Statins |
| title | sup 99m^Tc-cAbVCAM1-5 Imaging Is a Sensitive and Reproducible Tool for the Detection of Inflamed Atherosclerotic Lesions in Mice |
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