Induction, purification and characterization of malolactic enzyme from Oenococcus oeni SD-2a

The aim of this study was to purify a malolactic enzyme (MLE) from Oenococcus oeni (O. oeni) strain and determine its properties in detail. O. oeni SD-2a was cultivated in the ATB broth supplemented with 7 g/L L-malic acid for harvesting the cells. After harvest, the cells were washed and disrupted...

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Veröffentlicht in:	European food research & technology 2014-11, Vol.239 (5), p.827-835
Hauptverfasser:	Wang, Panxue, Li, Aixia, Dong, Mei, Fan, Mingtao
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Agriculture Analytical Chemistry Anion exchange anion exchange chromatography Biosynthesis Biotechnology Chemistry Chemistry and Materials Science Chromatography Enzymatic activity enzyme activity Enzymes Ethanol Factorial experiments Fermentation Food Food Science Forestry gel chromatography half life harvesting Life sciences manganese Mass spectrometry molecular weight Oenococcus oeni Original Paper Proteins Sodium chloride Studies temperature Wines
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