Modification of the brain proteome of Africanized honeybees (Apis mellifera) exposed to a sub‐lethal doses of the insecticide fipronil
Fipronil is a phenylpyrazole insecticide that is widely used in Brazilian agriculture for pest control. Although honeybees are not targets of fipronil, studies indicate that this pesticide can be harmful to honeybees. To assess the effects of fipronil in the brain of Africanized Apis mellifera worke...
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| Veröffentlicht in: | Ecotoxicology (London) 2014-11, Vol.23 (9), p.1659-1670 |
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| creator | Roat, T. C. dos Santos-Pinto, J. R. A. dos Santos, L. D. Santos, K. S. Malaspina, O. Palma, M. S. |
| description | Fipronil is a phenylpyrazole insecticide that is widely used in Brazilian agriculture for pest control. Although honeybees are not targets of fipronil, studies indicate that this pesticide can be harmful to honeybees. To assess the effects of fipronil in the brain of Africanized
Apis mellifera
workers, this study focused on the toxico-proteome profiling of the brain of newly emerged and aged honeybee workers that were exposed to a sub-lethal dose (10 pg fipronil per day. i.e.
1
/
100
of LD
50
/bee/day during 5 days) of the insecticide. Proteomic analysis identified 25 proteins that were differentially up-regulated or down-regulated when the fipronil-exposed and non-exposed groups were compared. These proteins are potentially related to pathogen susceptibility, neuronal chemical stress, neuronal protein misfolding, and occurrence of apoptosis, ischemia, visual impairment, damaged synapse formation, brain degeneration, memory and learning impairment. The exposure of honeybees to a very low dose of fipronil, even for a short period of time (5 days), was sufficient to cause a series of important neuroproteomic changes in the brains of honeybees. |
| doi_str_mv | 10.1007/s10646-014-1305-8 |
| format | Article |
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Apis mellifera
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1
/
100
of LD
50
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Apis mellifera
workers, this study focused on the toxico-proteome profiling of the brain of newly emerged and aged honeybee workers that were exposed to a sub-lethal dose (10 pg fipronil per day. i.e.
1
/
100
of LD
50
/bee/day during 5 days) of the insecticide. Proteomic analysis identified 25 proteins that were differentially up-regulated or down-regulated when the fipronil-exposed and non-exposed groups were compared. These proteins are potentially related to pathogen susceptibility, neuronal chemical stress, neuronal protein misfolding, and occurrence of apoptosis, ischemia, visual impairment, damaged synapse formation, brain degeneration, memory and learning impairment. The exposure of honeybees to a very low dose of fipronil, even for a short period of time (5 days), was sufficient to cause a series of important neuroproteomic changes in the brains of honeybees.</description><subject>Analysis</subject><subject>Animals</subject><subject>Bees</subject><subject>Brain - drug effects</subject><subject>Brain - metabolism</subject><subject>Brain damage</subject><subject>Brazil</subject><subject>Earth and Environmental Science</subject><subject>Ecology</subject><subject>Ecotoxicology</subject><subject>Environment</subject><subject>Environmental Management</subject><subject>Fipronil</subject><subject>Honeybee</subject><subject>Insecticides</subject><subject>Insecticides - toxicity</subject><subject>Lethal Dose 50</subject><subject>Occupational exposure</subject><subject>Pest control</subject><subject>Pesticides</subject><subject>Proteins</subject><subject>Proteome - drug effects</subject><subject>Pyrazoles - toxicity</subject><subject>Toxicity Tests, Subacute</subject><issn>0963-9292</issn><issn>1573-3017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kc1u1DAUhS1ERaeFB2CDLLGBRcq9sRPHy1EFFKkVG1hbjnPdcZXEg52RKCuWLHlGngSPpuVHAnlh6fo759g-jD1FOEMA9SojtLKtAGWFApqqe8BW2ChRCUD1kK1At6LSta6P2UnONwCglYRH7LhuUGgQsGLfruIQfHB2CXHm0fNlQ7xPNsx8m-JCcaL9dO1TYebwhQa-iTPd9kSZv1hvQ-YTjWPwlOxLTp-3MRdkidzyvOt_fP0-0rKxIx_KPN_7hzmTW4ILA3EfSs4cxsfsyNsx05O7_ZR9fPP6w_lFdfn-7bvz9WXlRKe6yqreylYNTQvgEazXHSB6cOBa3TWdsqidrIXQSF0NjRzICdfUVjp0Wllxyp4ffEvspx3lxdzEXZpLpMEWNLRQI_ymru1IJsw-Lsm6KWRn1gqFkloKUaizf1BlDTQFV77JhzL_S4AHgUsx50TebFOYbLo1CGZfqTlUakqlZl-p6Yrm2d2Fd_1Ewy_FfYcFqA9ALkfzNaU_XvRf158HKquC</recordid><startdate>201411</startdate><enddate>201411</enddate><creator>Roat, T. 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C.</au><au>dos Santos-Pinto, J. R. A.</au><au>dos Santos, L. D.</au><au>Santos, K. S.</au><au>Malaspina, O.</au><au>Palma, M. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modification of the brain proteome of Africanized honeybees (Apis mellifera) exposed to a sub‐lethal doses of the insecticide fipronil</atitle><jtitle>Ecotoxicology (London)</jtitle><stitle>Ecotoxicology</stitle><addtitle>Ecotoxicology</addtitle><date>2014-11</date><risdate>2014</risdate><volume>23</volume><issue>9</issue><spage>1659</spage><epage>1670</epage><pages>1659-1670</pages><issn>0963-9292</issn><eissn>1573-3017</eissn><coden>ECOTEL</coden><abstract>Fipronil is a phenylpyrazole insecticide that is widely used in Brazilian agriculture for pest control. Although honeybees are not targets of fipronil, studies indicate that this pesticide can be harmful to honeybees. To assess the effects of fipronil in the brain of Africanized
Apis mellifera
workers, this study focused on the toxico-proteome profiling of the brain of newly emerged and aged honeybee workers that were exposed to a sub-lethal dose (10 pg fipronil per day. i.e.
1
/
100
of LD
50
/bee/day during 5 days) of the insecticide. Proteomic analysis identified 25 proteins that were differentially up-regulated or down-regulated when the fipronil-exposed and non-exposed groups were compared. These proteins are potentially related to pathogen susceptibility, neuronal chemical stress, neuronal protein misfolding, and occurrence of apoptosis, ischemia, visual impairment, damaged synapse formation, brain degeneration, memory and learning impairment. The exposure of honeybees to a very low dose of fipronil, even for a short period of time (5 days), was sufficient to cause a series of important neuroproteomic changes in the brains of honeybees.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>25139030</pmid><doi>10.1007/s10646-014-1305-8</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0963-9292 |
| ispartof | Ecotoxicology (London), 2014-11, Vol.23 (9), p.1659-1670 |
| issn | 0963-9292 1573-3017 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Analysis Animals Bees Brain - drug effects Brain - metabolism Brain damage Brazil Earth and Environmental Science Ecology Ecotoxicology Environment Environmental Management Fipronil Honeybee Insecticides Insecticides - toxicity Lethal Dose 50 Occupational exposure Pest control Pesticides Proteins Proteome - drug effects Pyrazoles - toxicity Toxicity Tests, Subacute |
| title | Modification of the brain proteome of Africanized honeybees (Apis mellifera) exposed to a sub‐lethal doses of the insecticide fipronil |
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