A Comparison of the Lipid-lowering Effects of Four Different n-3 Highly Unsaturated Fatty Acids in HepG2 Cells
The effects on lipid metabolism of four different n-3 highly unsaturated fatty acids (n-3HUFA) including eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and tetracosahexaenoic acid (THA, 24:6n-3) were compared in the HepG2 cell model....
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| Veröffentlicht in: | Journal of Oleo Science 2014, Vol.63(10), pp.979-985 |
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| creator | Nagao, Koji Nakamitsu, Kazusa Ishida, Hiroki Yoshinaga, Kazuaki Nagai, Toshiharu Mizobe, Hoyo Kojima, Koichi Yanagita, Teruyoshi Beppu, Fumiaki Gotoh, Naohiro |
| description | The effects on lipid metabolism of four different n-3 highly unsaturated fatty acids (n-3HUFA) including eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and tetracosahexaenoic acid (THA, 24:6n-3) were compared in the HepG2 cell model. None of the n-3HUFAs affected the viability of the cells. THA exerted the strongest suppression on the synthesis of triacylglycerol and cholesteryl ester (ChE), and the order of the strength of suppression was found to be THA > DHA > DPA > EPA. The mRNA level of fatty acid synthase was suppressed by the n-3HUFAs and the order of the strength of suppression by n-3HUFAs was the same in both triacylglycerol and ChE synthesis. These findings support previous animal test results using EPA, DPA, and DHA. In conclusion, both the number of carbon atoms and double bonds in an n-3HUFA structure has an effect on lipid metabolism in HepG2 cells. |
| doi_str_mv | 10.5650/jos.ess14118 |
| format | Article |
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None of the n-3HUFAs affected the viability of the cells. THA exerted the strongest suppression on the synthesis of triacylglycerol and cholesteryl ester (ChE), and the order of the strength of suppression was found to be THA > DHA > DPA > EPA. The mRNA level of fatty acid synthase was suppressed by the n-3HUFAs and the order of the strength of suppression by n-3HUFAs was the same in both triacylglycerol and ChE synthesis. These findings support previous animal test results using EPA, DPA, and DHA. In conclusion, both the number of carbon atoms and double bonds in an n-3HUFA structure has an effect on lipid metabolism in HepG2 cells.</description><identifier>ISSN: 1345-8957</identifier><identifier>EISSN: 1347-3352</identifier><identifier>DOI: 10.5650/jos.ess14118</identifier><identifier>PMID: 25213447</identifier><language>eng</language><publisher>Japan: Japan Oil Chemists' Society</publisher><subject>Cell Survival - drug effects ; Cholesterol Esters - biosynthesis ; Depression, Chemical ; docosahexaenoic acid ; Docosahexaenoic Acids - chemistry ; Docosahexaenoic Acids - pharmacology ; docosapentaenoic acid ; eicosapentaenoic acid ; Eicosapentaenoic Acid - chemistry ; Eicosapentaenoic Acid - pharmacology ; Fatty Acid Synthases - genetics ; Fatty Acid Synthases - metabolism ; Fatty Acids, Omega-3 - chemistry ; Fatty Acids, Omega-3 - pharmacology ; Fatty Acids, Unsaturated - chemistry ; Fatty Acids, Unsaturated - pharmacology ; Hep G2 Cells ; HepG2 cell ; Humans ; lipid metabolism ; Lipid Metabolism - drug effects ; n-3 highly unsaturated fatty acid ; RNA, Messenger - metabolism ; Structure-Activity Relationship ; tetracosahexaenoic acid ; Triglycerides - biosynthesis</subject><ispartof>Journal of Oleo Science, 2014, Vol.63(10), pp.979-985</ispartof><rights>2014 by Japan Oil Chemists' Society</rights><rights>Copyright Japan Science and Technology Agency 2014</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c622t-5961ebe7b4741e8451c2b822cd42fd02e776501f0716f3581d61cc6e47ef26f23</citedby><cites>FETCH-LOGICAL-c622t-5961ebe7b4741e8451c2b822cd42fd02e776501f0716f3581d61cc6e47ef26f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25213447$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nagao, Koji</creatorcontrib><creatorcontrib>Nakamitsu, Kazusa</creatorcontrib><creatorcontrib>Ishida, Hiroki</creatorcontrib><creatorcontrib>Yoshinaga, Kazuaki</creatorcontrib><creatorcontrib>Nagai, Toshiharu</creatorcontrib><creatorcontrib>Mizobe, Hoyo</creatorcontrib><creatorcontrib>Kojima, Koichi</creatorcontrib><creatorcontrib>Yanagita, Teruyoshi</creatorcontrib><creatorcontrib>Beppu, Fumiaki</creatorcontrib><creatorcontrib>Gotoh, Naohiro</creatorcontrib><creatorcontrib>Department of Food Science and Technology</creatorcontrib><creatorcontrib>Saga University</creatorcontrib><creatorcontrib>Department of Health and Nutrition Science</creatorcontrib><creatorcontrib>Nishikyushu University</creatorcontrib><creatorcontrib>Department of Applied Biochemistry and Food Science</creatorcontrib><creatorcontrib>Tokyo University of Marine Science and Technology</creatorcontrib><creatorcontrib>Tsukishima Foods industry Co.Ltd</creatorcontrib><title>A Comparison of the Lipid-lowering Effects of Four Different n-3 Highly Unsaturated Fatty Acids in HepG2 Cells</title><title>Journal of Oleo Science</title><addtitle>J Oleo Sci</addtitle><description>The effects on lipid metabolism of four different n-3 highly unsaturated fatty acids (n-3HUFA) including eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and tetracosahexaenoic acid (THA, 24:6n-3) were compared in the HepG2 cell model. None of the n-3HUFAs affected the viability of the cells. THA exerted the strongest suppression on the synthesis of triacylglycerol and cholesteryl ester (ChE), and the order of the strength of suppression was found to be THA > DHA > DPA > EPA. The mRNA level of fatty acid synthase was suppressed by the n-3HUFAs and the order of the strength of suppression by n-3HUFAs was the same in both triacylglycerol and ChE synthesis. These findings support previous animal test results using EPA, DPA, and DHA. In conclusion, both the number of carbon atoms and double bonds in an n-3HUFA structure has an effect on lipid metabolism in HepG2 cells.</description><subject>Cell Survival - drug effects</subject><subject>Cholesterol Esters - biosynthesis</subject><subject>Depression, Chemical</subject><subject>docosahexaenoic acid</subject><subject>Docosahexaenoic Acids - chemistry</subject><subject>Docosahexaenoic Acids - pharmacology</subject><subject>docosapentaenoic acid</subject><subject>eicosapentaenoic acid</subject><subject>Eicosapentaenoic Acid - chemistry</subject><subject>Eicosapentaenoic Acid - pharmacology</subject><subject>Fatty Acid Synthases - genetics</subject><subject>Fatty Acid Synthases - metabolism</subject><subject>Fatty Acids, Omega-3 - chemistry</subject><subject>Fatty Acids, Omega-3 - pharmacology</subject><subject>Fatty Acids, Unsaturated - chemistry</subject><subject>Fatty Acids, Unsaturated - pharmacology</subject><subject>Hep G2 Cells</subject><subject>HepG2 cell</subject><subject>Humans</subject><subject>lipid metabolism</subject><subject>Lipid Metabolism - drug effects</subject><subject>n-3 highly unsaturated fatty acid</subject><subject>RNA, Messenger - metabolism</subject><subject>Structure-Activity Relationship</subject><subject>tetracosahexaenoic acid</subject><subject>Triglycerides - biosynthesis</subject><issn>1345-8957</issn><issn>1347-3352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUUFv2yAYRdWmtmt723lC2nXuABuwj5HXJJMi7bKeLYI_EiwHPCCa8u-Hmya7fKDve7z3eB9Cnyl55oKT74OPzxAjrSitb9A9LStZlCVnH97uvKgbLu_QpxgHQnKfy1t0xzjLs0reI7fArT9MKtjoHfYGpz3gjZ1sX4z-LwTrdvjFGNApztOlPwb8w-ZGAJewK0q8trv9eMKvLqp0DCpBj5cqpRNeaNtHbB1ew7RiuIVxjI_oo1FjhKf38wG9Ll9-t-ti82v1s11sCi0YSwVvBIUtyG0lKwp1xalm25ox3VfM9ISBlPnr1BBJhSl5TXtBtRZQSTBMGFY-oK9n3in4P0eIqRuyc5clO8qFaHIAfEZ9O6N08DEGMN0U7EGFU0dJN4ebX8XuEm6Gf3knPW4P0F_BlzQzYHUG5KnVavRutA7-S_eD9CNkSkZo1REiyqyTt9KRRja51NkTyzubpdoz0xCT2sFVSoVk9QhvvkQ528z1YvA61XsVOnDlP3rOoxc</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Nagao, Koji</creator><creator>Nakamitsu, Kazusa</creator><creator>Ishida, Hiroki</creator><creator>Yoshinaga, Kazuaki</creator><creator>Nagai, Toshiharu</creator><creator>Mizobe, Hoyo</creator><creator>Kojima, Koichi</creator><creator>Yanagita, Teruyoshi</creator><creator>Beppu, Fumiaki</creator><creator>Gotoh, Naohiro</creator><general>Japan Oil Chemists' Society</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>20141001</creationdate><title>A Comparison of the Lipid-lowering Effects of Four Different n-3 Highly Unsaturated Fatty Acids in HepG2 Cells</title><author>Nagao, Koji ; Nakamitsu, Kazusa ; Ishida, Hiroki ; Yoshinaga, Kazuaki ; Nagai, Toshiharu ; Mizobe, Hoyo ; Kojima, Koichi ; Yanagita, Teruyoshi ; Beppu, Fumiaki ; Gotoh, Naohiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c622t-5961ebe7b4741e8451c2b822cd42fd02e776501f0716f3581d61cc6e47ef26f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Cell Survival - drug effects</topic><topic>Cholesterol Esters - biosynthesis</topic><topic>Depression, Chemical</topic><topic>docosahexaenoic acid</topic><topic>Docosahexaenoic Acids - chemistry</topic><topic>Docosahexaenoic Acids - pharmacology</topic><topic>docosapentaenoic acid</topic><topic>eicosapentaenoic acid</topic><topic>Eicosapentaenoic Acid - chemistry</topic><topic>Eicosapentaenoic Acid - pharmacology</topic><topic>Fatty Acid Synthases - genetics</topic><topic>Fatty Acid Synthases - metabolism</topic><topic>Fatty Acids, Omega-3 - chemistry</topic><topic>Fatty Acids, Omega-3 - pharmacology</topic><topic>Fatty Acids, Unsaturated - chemistry</topic><topic>Fatty Acids, Unsaturated - pharmacology</topic><topic>Hep G2 Cells</topic><topic>HepG2 cell</topic><topic>Humans</topic><topic>lipid metabolism</topic><topic>Lipid Metabolism - drug effects</topic><topic>n-3 highly unsaturated fatty acid</topic><topic>RNA, Messenger - metabolism</topic><topic>Structure-Activity Relationship</topic><topic>tetracosahexaenoic acid</topic><topic>Triglycerides - biosynthesis</topic><toplevel>online_resources</toplevel><creatorcontrib>Nagao, Koji</creatorcontrib><creatorcontrib>Nakamitsu, Kazusa</creatorcontrib><creatorcontrib>Ishida, Hiroki</creatorcontrib><creatorcontrib>Yoshinaga, Kazuaki</creatorcontrib><creatorcontrib>Nagai, Toshiharu</creatorcontrib><creatorcontrib>Mizobe, Hoyo</creatorcontrib><creatorcontrib>Kojima, Koichi</creatorcontrib><creatorcontrib>Yanagita, Teruyoshi</creatorcontrib><creatorcontrib>Beppu, Fumiaki</creatorcontrib><creatorcontrib>Gotoh, Naohiro</creatorcontrib><creatorcontrib>Department of Food Science and Technology</creatorcontrib><creatorcontrib>Saga University</creatorcontrib><creatorcontrib>Department of Health and Nutrition Science</creatorcontrib><creatorcontrib>Nishikyushu University</creatorcontrib><creatorcontrib>Department of Applied Biochemistry and Food Science</creatorcontrib><creatorcontrib>Tokyo University of Marine Science and Technology</creatorcontrib><creatorcontrib>Tsukishima Foods industry Co.Ltd</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of Oleo Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagao, Koji</au><au>Nakamitsu, Kazusa</au><au>Ishida, Hiroki</au><au>Yoshinaga, Kazuaki</au><au>Nagai, Toshiharu</au><au>Mizobe, Hoyo</au><au>Kojima, Koichi</au><au>Yanagita, Teruyoshi</au><au>Beppu, Fumiaki</au><au>Gotoh, Naohiro</au><aucorp>Department of Food Science and Technology</aucorp><aucorp>Saga University</aucorp><aucorp>Department of Health and Nutrition Science</aucorp><aucorp>Nishikyushu University</aucorp><aucorp>Department of Applied Biochemistry and Food Science</aucorp><aucorp>Tokyo University of Marine Science and Technology</aucorp><aucorp>Tsukishima Foods industry Co.Ltd</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Comparison of the Lipid-lowering Effects of Four Different n-3 Highly Unsaturated Fatty Acids in HepG2 Cells</atitle><jtitle>Journal of Oleo Science</jtitle><addtitle>J Oleo Sci</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>63</volume><issue>10</issue><spage>979</spage><epage>985</epage><pages>979-985</pages><issn>1345-8957</issn><eissn>1347-3352</eissn><abstract>The effects on lipid metabolism of four different n-3 highly unsaturated fatty acids (n-3HUFA) including eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and tetracosahexaenoic acid (THA, 24:6n-3) were compared in the HepG2 cell model. None of the n-3HUFAs affected the viability of the cells. THA exerted the strongest suppression on the synthesis of triacylglycerol and cholesteryl ester (ChE), and the order of the strength of suppression was found to be THA > DHA > DPA > EPA. The mRNA level of fatty acid synthase was suppressed by the n-3HUFAs and the order of the strength of suppression by n-3HUFAs was the same in both triacylglycerol and ChE synthesis. These findings support previous animal test results using EPA, DPA, and DHA. In conclusion, both the number of carbon atoms and double bonds in an n-3HUFA structure has an effect on lipid metabolism in HepG2 cells.</abstract><cop>Japan</cop><pub>Japan Oil Chemists' Society</pub><pmid>25213447</pmid><doi>10.5650/jos.ess14118</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Cell Survival - drug effects Cholesterol Esters - biosynthesis Depression, Chemical docosahexaenoic acid Docosahexaenoic Acids - chemistry Docosahexaenoic Acids - pharmacology docosapentaenoic acid eicosapentaenoic acid Eicosapentaenoic Acid - chemistry Eicosapentaenoic Acid - pharmacology Fatty Acid Synthases - genetics Fatty Acid Synthases - metabolism Fatty Acids, Omega-3 - chemistry Fatty Acids, Omega-3 - pharmacology Fatty Acids, Unsaturated - chemistry Fatty Acids, Unsaturated - pharmacology Hep G2 Cells HepG2 cell Humans lipid metabolism Lipid Metabolism - drug effects n-3 highly unsaturated fatty acid RNA, Messenger - metabolism Structure-Activity Relationship tetracosahexaenoic acid Triglycerides - biosynthesis |
| title | A Comparison of the Lipid-lowering Effects of Four Different n-3 Highly Unsaturated Fatty Acids in HepG2 Cells |
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