Simultaneous Quantification of Glibenclamide, Simvastatin, and Quercetin by Using LC-UV Method and Its Application to Pharmacokinetic Study in Rats
A sensitive, precise, and simple LC method for the simultaneous quantification of glibenclamide, simvastatin, and quercetin in rat plasma has been developed and validated. The chromatographic separation was achieved on a cyano column (250 mm × 4.6 mm, 5 µm) maintained at room temperature, using isoc...
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| Veröffentlicht in: | Chromatography research international 2013-01, Vol.2013 (2013), p.1-6 |
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| container_title | Chromatography research international |
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| creator | Dash, Ranjeet Prasad Duppala, Vijay Jivrajani, Mehul N. Thakur, Sandeep Kumar Ravat, Nirav M. Nivsarkar, Manish |
| description | A sensitive, precise, and simple LC method for the simultaneous quantification of glibenclamide, simvastatin, and quercetin in rat plasma has been developed and validated. The chromatographic separation was achieved on a cyano column (250 mm × 4.6 mm, 5 µm) maintained at room temperature, using isocratic elution with methanol : acetonitrile : 10 mM potassium dihydrogen orthophosphate, pH adjusted to 4.5 with o-phosphoric acid (8 : 32 : 60, v/v) and detected using UV-VIS detector. Plasma samples were deproteinated with 0.1% perchloric acid and acetonitrile for extraction of the glibenclamide, simvastatin, and quercetin which resulted in their high recoveries. LC calibration curves based on the extracts from the rat plasma were linear in the range of 50–1000 ng mL−1 for all the three drugs. The limit of quantification was 50 ng mL−1. The described method was successfully applied to study the pharmacokinetics of glibenclamide, simvastatin, and quercetin following oral administration, in combination to Sprague-Dawley rats. |
| doi_str_mv | 10.1155/2013/768160 |
| format | Article |
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The chromatographic separation was achieved on a cyano column (250 mm × 4.6 mm, 5 µm) maintained at room temperature, using isocratic elution with methanol : acetonitrile : 10 mM potassium dihydrogen orthophosphate, pH adjusted to 4.5 with o-phosphoric acid (8 : 32 : 60, v/v) and detected using UV-VIS detector. Plasma samples were deproteinated with 0.1% perchloric acid and acetonitrile for extraction of the glibenclamide, simvastatin, and quercetin which resulted in their high recoveries. LC calibration curves based on the extracts from the rat plasma were linear in the range of 50–1000 ng mL−1 for all the three drugs. The limit of quantification was 50 ng mL−1. The described method was successfully applied to study the pharmacokinetics of glibenclamide, simvastatin, and quercetin following oral administration, in combination to Sprague-Dawley rats.</description><identifier>ISSN: 2090-3502</identifier><identifier>EISSN: 2090-3510</identifier><identifier>DOI: 10.1155/2013/768160</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Accuracy ; Bioavailability ; Chromatography ; Diabetes ; Drug dosages ; Health education ; Pharmaceutical sciences ; Pharmaceuticals ; Plasma ; Potassium ; Vortices</subject><ispartof>Chromatography research international, 2013-01, Vol.2013 (2013), p.1-6</ispartof><rights>Copyright © 2013 Vijay Duppala et al.</rights><rights>Copyright © 2013 Vijay Duppala et al. Vijay Duppala et al. 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The described method was successfully applied to study the pharmacokinetics of glibenclamide, simvastatin, and quercetin following oral administration, in combination to Sprague-Dawley rats.</description><subject>Accuracy</subject><subject>Bioavailability</subject><subject>Chromatography</subject><subject>Diabetes</subject><subject>Drug dosages</subject><subject>Health education</subject><subject>Pharmaceutical sciences</subject><subject>Pharmaceuticals</subject><subject>Plasma</subject><subject>Potassium</subject><subject>Vortices</subject><issn>2090-3502</issn><issn>2090-3510</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqF0E9vFCEYBvCJ0cSm9uTZhMSbdtoXGJjh2Gy0NtmmtnW9Tt7lj0udhXVgNPs5-oWlTturXIDwex-Sp6reUjihVIhTBpSftrKjEl5UBwwU1FxQePl8Bva6OkrpDsoSrVJtc1Dd3_rtNGQMNk6JXE8YsndeY_YxkOjI-eDXNugBt97YY1L0b0y5PIdjgsGUCTtqW65kvSer5MMPslzUq-_k0uZNNP_MRU7kbLcbnmJzJF83OG5Rx58-lGFNbvNk9qSk3GBOb6pXDodkjx73w2r1-dO3xZd6eXV-sThb1shYC3XDnWGGa-jUWkqtuRbYsFaiaU2jrADJQQmpWOdgbdF1AIxp4ZgwLXZo-GH1fs7djfHXZFPu7-I0hvJlT4WUgioFXVEfZ6XHmNJoXb8b_RbHfU-hfyi-fyi-n4sv-sOsNz4Y_OP_g9_N2BZiHT7jRrWMcv4XRdeMhg</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Dash, Ranjeet Prasad</creator><creator>Duppala, Vijay</creator><creator>Jivrajani, Mehul N.</creator><creator>Thakur, Sandeep Kumar</creator><creator>Ravat, Nirav M.</creator><creator>Nivsarkar, Manish</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20130101</creationdate><title>Simultaneous Quantification of Glibenclamide, Simvastatin, and Quercetin by Using LC-UV Method and Its Application to Pharmacokinetic Study in Rats</title><author>Dash, Ranjeet Prasad ; 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The chromatographic separation was achieved on a cyano column (250 mm × 4.6 mm, 5 µm) maintained at room temperature, using isocratic elution with methanol : acetonitrile : 10 mM potassium dihydrogen orthophosphate, pH adjusted to 4.5 with o-phosphoric acid (8 : 32 : 60, v/v) and detected using UV-VIS detector. Plasma samples were deproteinated with 0.1% perchloric acid and acetonitrile for extraction of the glibenclamide, simvastatin, and quercetin which resulted in their high recoveries. LC calibration curves based on the extracts from the rat plasma were linear in the range of 50–1000 ng mL−1 for all the three drugs. The limit of quantification was 50 ng mL−1. 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| subjects | Accuracy Bioavailability Chromatography Diabetes Drug dosages Health education Pharmaceutical sciences Pharmaceuticals Plasma Potassium Vortices |
| title | Simultaneous Quantification of Glibenclamide, Simvastatin, and Quercetin by Using LC-UV Method and Its Application to Pharmacokinetic Study in Rats |
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