Preparation of reversibly immobilized Jack bean urease on microchannel surface and application for enzyme inhibition assay

We have developed a sensitive enzyme inhibition assay on microfluidic system. The analysis was carried out by immobilizing enzyme through amide-bond or disulfide-bond formation with surface. Followed by detection of reaction product through fluorescent density were evaluated reusability, stability a...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Microfluidics and nanofluidics 2014-10, Vol.17 (4), p.721-728
Hauptverfasser:	Tang, Xiuwen, Liu, Sufang, Wang, Sifeng, Zhang, Qin, Cheng, Zhiyi
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Bacteriology Bioassays Biological and medical sciences Biomedical Engineering and Bioengineering Biotechnology Engineering Engineering Fluid Dynamics Enzymatic activity Enzyme inhibitors Fundamental and applied biological sciences. Psychology Metabolism. Enzymes Microbiology Nanotechnology and Microengineering Research Paper
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!