Confocal Raman spectroscopy to monitor intracellular penetration of TiO2 nanoparticles
Confocal Raman microscopy, a noninvasive, label‐free, and high‐spatial resolution imaging technique, in combination with K‐mean cluster analysis and a correlation coefficient map, was employed to trace titanium dioxide (TiO2) nanoparticles in living MCF‐7 and TERT cells. The penetration of TiO2 nano...
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| Veröffentlicht in: | Journal of Raman spectroscopy 2014-09, Vol.45 (9), p.807-813 |
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| creator | Salehi, Hamideh Calas-Bennasar, Isabelle Durand, Jean-Cédric Middendorp, Elodie Valcarcel, Jean Larroque, Christian Nagy, Katalin Turzó K, Kinga Dekany, Imre Cuisinier, Frédéric J. G. |
| description | Confocal Raman microscopy, a noninvasive, label‐free, and high‐spatial resolution imaging technique, in combination with K‐mean cluster analysis and a correlation coefficient map, was employed to trace titanium dioxide (TiO2) nanoparticles in living MCF‐7 and TERT cells. The penetration of TiO2 nanoparticles into cells revealed a gradual time‐dependent diffusion of nanoparticles over the entire cell. Cell apoptosis was monitored by tracing cytochrome c diffusion into the cytoplasm. A comparison with the mitochondrial clustering indicated that cytochrome c was inside the mitochondria for TiO2 concentration of 2 µg ml−1. This result demonstrates that the presence of TiO2 particles within a cell does not induce apoptosis. We demonstrated that confocal Raman microscopy allow to follow penetration of TiO2 particles in cell and to monitor the apoptotic status of the penetrated cells. Copyright © 2014 John Wiley & Sons, Ltd.
Titanium dioxide (TiO2) nanoparticles in living MCF‐7 and TERT cells were traced by confocal Raman microscopy in combination with K‐mean cluster analysis and a correlation coefficient. A gradual time‐dependent diffusion of nanoparticles over the entire cell was observed. Cell apoptosis was monitored by tracing cytochrome c diffusion from mitochondria into the cytoplasm. The concentration of 2 µg ml−1 TiO2 particles within a cell does not induce apoptosis. |
| doi_str_mv | 10.1002/jrs.4561 |
| format | Article |
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Titanium dioxide (TiO2) nanoparticles in living MCF‐7 and TERT cells were traced by confocal Raman microscopy in combination with K‐mean cluster analysis and a correlation coefficient. A gradual time‐dependent diffusion of nanoparticles over the entire cell was observed. Cell apoptosis was monitored by tracing cytochrome c diffusion from mitochondria into the cytoplasm. The concentration of 2 µg ml−1 TiO2 particles within a cell does not induce apoptosis.</description><identifier>ISSN: 0377-0486</identifier><identifier>EISSN: 1097-4555</identifier><identifier>DOI: 10.1002/jrs.4561</identifier><identifier>CODEN: JRSPAF</identifier><language>eng</language><publisher>Bognor Regis: Blackwell Publishing Ltd</publisher><subject>cell imaging ; correlation coefficient ; K-mean cluster ; Raman microscopy ; titanium dioxide</subject><ispartof>Journal of Raman spectroscopy, 2014-09, Vol.45 (9), p.807-813</ispartof><rights>Copyright © 2014 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjrs.4561$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjrs.4561$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Salehi, Hamideh</creatorcontrib><creatorcontrib>Calas-Bennasar, Isabelle</creatorcontrib><creatorcontrib>Durand, Jean-Cédric</creatorcontrib><creatorcontrib>Middendorp, Elodie</creatorcontrib><creatorcontrib>Valcarcel, Jean</creatorcontrib><creatorcontrib>Larroque, Christian</creatorcontrib><creatorcontrib>Nagy, Katalin</creatorcontrib><creatorcontrib>Turzó K, Kinga</creatorcontrib><creatorcontrib>Dekany, Imre</creatorcontrib><creatorcontrib>Cuisinier, Frédéric J. G.</creatorcontrib><title>Confocal Raman spectroscopy to monitor intracellular penetration of TiO2 nanoparticles</title><title>Journal of Raman spectroscopy</title><addtitle>J. Raman Spectrosc</addtitle><description>Confocal Raman microscopy, a noninvasive, label‐free, and high‐spatial resolution imaging technique, in combination with K‐mean cluster analysis and a correlation coefficient map, was employed to trace titanium dioxide (TiO2) nanoparticles in living MCF‐7 and TERT cells. The penetration of TiO2 nanoparticles into cells revealed a gradual time‐dependent diffusion of nanoparticles over the entire cell. Cell apoptosis was monitored by tracing cytochrome c diffusion into the cytoplasm. A comparison with the mitochondrial clustering indicated that cytochrome c was inside the mitochondria for TiO2 concentration of 2 µg ml−1. This result demonstrates that the presence of TiO2 particles within a cell does not induce apoptosis. We demonstrated that confocal Raman microscopy allow to follow penetration of TiO2 particles in cell and to monitor the apoptotic status of the penetrated cells. Copyright © 2014 John Wiley & Sons, Ltd.
Titanium dioxide (TiO2) nanoparticles in living MCF‐7 and TERT cells were traced by confocal Raman microscopy in combination with K‐mean cluster analysis and a correlation coefficient. A gradual time‐dependent diffusion of nanoparticles over the entire cell was observed. Cell apoptosis was monitored by tracing cytochrome c diffusion from mitochondria into the cytoplasm. The concentration of 2 µg ml−1 TiO2 particles within a cell does not induce apoptosis.</description><subject>cell imaging</subject><subject>correlation coefficient</subject><subject>K-mean cluster</subject><subject>Raman microscopy</subject><subject>titanium dioxide</subject><issn>0377-0486</issn><issn>1097-4555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNpFkE1LAzEURYMoWKvgTwi4Hn357iylWLWUFmqty5CGBFKnyZhM0f57pyi6urzL4V04CF0TuCUA9G6byy0XkpygAYFaVVwIcYoGwJSqgI_kObooZQsAdS3JAK3HKfpkTYOXZmciLq2zXU7FpvaAu4R3KYYuZRxil411TbNvTMati66_u5AiTh6vwoLiaGJqTe6CbVy5RGfeNMVd_eYQvU4eVuOnarZ4fB7fz6pAa0Yq5qySykguasGEFMZbw_vSUHDSW07oyHujqHNiA4x5b2u_8VQQDsYqADZENz9_25w-9q50epv2OfaTmvQShGQUSE9VP9RnaNxBtznsTD5oAvqoTPfK9FGZni5fjvnPh9K5rz_e5HctFVNCv80f9XI6ZxM-W-s5-wYZ6nDl</recordid><startdate>201409</startdate><enddate>201409</enddate><creator>Salehi, Hamideh</creator><creator>Calas-Bennasar, Isabelle</creator><creator>Durand, Jean-Cédric</creator><creator>Middendorp, Elodie</creator><creator>Valcarcel, Jean</creator><creator>Larroque, Christian</creator><creator>Nagy, Katalin</creator><creator>Turzó K, Kinga</creator><creator>Dekany, Imre</creator><creator>Cuisinier, Frédéric J. 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G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Confocal Raman spectroscopy to monitor intracellular penetration of TiO2 nanoparticles</atitle><jtitle>Journal of Raman spectroscopy</jtitle><addtitle>J. Raman Spectrosc</addtitle><date>2014-09</date><risdate>2014</risdate><volume>45</volume><issue>9</issue><spage>807</spage><epage>813</epage><pages>807-813</pages><issn>0377-0486</issn><eissn>1097-4555</eissn><coden>JRSPAF</coden><abstract>Confocal Raman microscopy, a noninvasive, label‐free, and high‐spatial resolution imaging technique, in combination with K‐mean cluster analysis and a correlation coefficient map, was employed to trace titanium dioxide (TiO2) nanoparticles in living MCF‐7 and TERT cells. The penetration of TiO2 nanoparticles into cells revealed a gradual time‐dependent diffusion of nanoparticles over the entire cell. Cell apoptosis was monitored by tracing cytochrome c diffusion into the cytoplasm. A comparison with the mitochondrial clustering indicated that cytochrome c was inside the mitochondria for TiO2 concentration of 2 µg ml−1. This result demonstrates that the presence of TiO2 particles within a cell does not induce apoptosis. We demonstrated that confocal Raman microscopy allow to follow penetration of TiO2 particles in cell and to monitor the apoptotic status of the penetrated cells. Copyright © 2014 John Wiley & Sons, Ltd.
Titanium dioxide (TiO2) nanoparticles in living MCF‐7 and TERT cells were traced by confocal Raman microscopy in combination with K‐mean cluster analysis and a correlation coefficient. A gradual time‐dependent diffusion of nanoparticles over the entire cell was observed. Cell apoptosis was monitored by tracing cytochrome c diffusion from mitochondria into the cytoplasm. The concentration of 2 µg ml−1 TiO2 particles within a cell does not induce apoptosis.</abstract><cop>Bognor Regis</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1002/jrs.4561</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | cell imaging correlation coefficient K-mean cluster Raman microscopy titanium dioxide |
| title | Confocal Raman spectroscopy to monitor intracellular penetration of TiO2 nanoparticles |
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