Establishment ofSchistosoma japonicumcalpain-specific mouse T cell hybridomas and identification of a T cell epitope that stimulates IFN[gamma] production
Calpain is a calcium-dependent cystein protease, and the homologues of schistosome are known as one of vaccine candidate molecules against schistosomiasis. Here, we established two IL-2 producing T cell hybridoma cell lines specific forSchistosoma japonicumcalpain, to identify T cell epitope(s) on t...
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| Veröffentlicht in: | Vaccine 2005-04, Vol.23 (21), p.2813 |
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| container_title | Vaccine |
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| creator | Osada, Yoshio Kumagai, Takashi Hato, Mariko Suzuki, Takashi El-Malky, Mohamed Asahi, Hiroko Kanazawa, Tamotsu Ohta, Nobuo |
| description | Calpain is a calcium-dependent cystein protease, and the homologues of schistosome are known as one of vaccine candidate molecules against schistosomiasis. Here, we established two IL-2 producing T cell hybridoma cell lines specific forSchistosoma japonicumcalpain, to identify T cell epitope(s) on the molecule. Overlapping 15mer oligopeptides of calpain were synthesized and tested for their stimulatory abilities to the hybridomas. As a result, epitopes recognized by the two hybridoma lines were the same: EQLKIYAQRC. Spleen cells from calpain multiple antigenic peptide (MAP)-immunized BALB/c mice produced IFNγ upon stimulation with MAP or soluble worm antigen preparation (SWAP). The identification of the T cell epitope to stimulate Th1 response will contribute to the proper design of synthetic vaccines, evaluation of their protective potentials and elucidation of protective mechanisms in murine experimental schistosomiasis. |
| doi_str_mv | 10.1016/j.vaccine.2004.10.042 |
| format | Article |
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Here, we established two IL-2 producing T cell hybridoma cell lines specific forSchistosoma japonicumcalpain, to identify T cell epitope(s) on the molecule. Overlapping 15mer oligopeptides of calpain were synthesized and tested for their stimulatory abilities to the hybridomas. As a result, epitopes recognized by the two hybridoma lines were the same: EQLKIYAQRC. Spleen cells from calpain multiple antigenic peptide (MAP)-immunized BALB/c mice produced IFNγ upon stimulation with MAP or soluble worm antigen preparation (SWAP). The identification of the T cell epitope to stimulate Th1 response will contribute to the proper design of synthetic vaccines, evaluation of their protective potentials and elucidation of protective mechanisms in murine experimental schistosomiasis.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2004.10.042</identifier><language>eng</language><publisher>Kidlington: Elsevier Limited</publisher><subject>Experiments ; Immunization ; Infections ; Laboratories ; Lymphocytes ; Mollusks ; Peptides ; Proteins ; Schistosomiasis ; Tropical diseases ; Vaccines</subject><ispartof>Vaccine, 2005-04, Vol.23 (21), p.2813</ispartof><rights>Copyright Elsevier Limited Apr 15, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1559074411?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,64385,64389,72469</link.rule.ids></links><search><creatorcontrib>Osada, Yoshio</creatorcontrib><creatorcontrib>Kumagai, Takashi</creatorcontrib><creatorcontrib>Hato, Mariko</creatorcontrib><creatorcontrib>Suzuki, Takashi</creatorcontrib><creatorcontrib>El-Malky, Mohamed</creatorcontrib><creatorcontrib>Asahi, Hiroko</creatorcontrib><creatorcontrib>Kanazawa, Tamotsu</creatorcontrib><creatorcontrib>Ohta, Nobuo</creatorcontrib><title>Establishment ofSchistosoma japonicumcalpain-specific mouse T cell hybridomas and identification of a T cell epitope that stimulates IFN[gamma] production</title><title>Vaccine</title><description>Calpain is a calcium-dependent cystein protease, and the homologues of schistosome are known as one of vaccine candidate molecules against schistosomiasis. 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Here, we established two IL-2 producing T cell hybridoma cell lines specific forSchistosoma japonicumcalpain, to identify T cell epitope(s) on the molecule. Overlapping 15mer oligopeptides of calpain were synthesized and tested for their stimulatory abilities to the hybridomas. As a result, epitopes recognized by the two hybridoma lines were the same: EQLKIYAQRC. Spleen cells from calpain multiple antigenic peptide (MAP)-immunized BALB/c mice produced IFNγ upon stimulation with MAP or soluble worm antigen preparation (SWAP). The identification of the T cell epitope to stimulate Th1 response will contribute to the proper design of synthetic vaccines, evaluation of their protective potentials and elucidation of protective mechanisms in murine experimental schistosomiasis.</abstract><cop>Kidlington</cop><pub>Elsevier Limited</pub><doi>10.1016/j.vaccine.2004.10.042</doi></addata></record> |
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| source | ScienceDirect Journals (5 years ago - present); ProQuest Central UK/Ireland |
| subjects | Experiments Immunization Infections Laboratories Lymphocytes Mollusks Peptides Proteins Schistosomiasis Tropical diseases Vaccines |
| title | Establishment ofSchistosoma japonicumcalpain-specific mouse T cell hybridomas and identification of a T cell epitope that stimulates IFN[gamma] production |
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