Heterologous protection in pigs induced by a plasmid-cured andcrpgene-deletedSalmonella choleraesuislive vaccine
In this study, we exploited acrp(cAMP receptor protein) gene-deleted, virulence plasmid-curedSalmonella choleraesuismutant with decreased carbon source utilization, designatedS.C.-Δcrp/vpl-, as a live vaccine strain. Normal weight gain with no clinical signs was observed in pigs immunized with high...
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| Veröffentlicht in: | Vaccine 2007-10, Vol.25 (41), p.7031 |
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| container_issue | 41 |
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| container_title | Vaccine |
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| creator | Chu, Chun-Yen Wang, Shiang-Yiu Chen, Zeng-Weng Chien, Maw-Sheng Huang, Ji-Ping Chen, Jen-Jin Hong, Li-Shian Shiau, Ai-Li Tsai, Jeng-Liang Wu, Chao-Liang |
| description | In this study, we exploited acrp(cAMP receptor protein) gene-deleted, virulence plasmid-curedSalmonella choleraesuismutant with decreased carbon source utilization, designatedS.C.-Δcrp/vpl-, as a live vaccine strain. Normal weight gain with no clinical signs was observed in pigs immunized with high doses ofS.C.-Δcrp/vpl-live vaccine. Vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets fromSalmonellainfection. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Furthermore, the peripheral blood mononuclear cells from immunized piglets also developedSalmonellaspecific T-cell proliferative responsein vitro. Our results indicate that immunogenic antigens inS.C.-Δcrp/vpl-can induce adequate immunity to protect pigs against challenge with a heterologous virulent strain. Thus, this mutant holds promise for the development of a new liveS. choleraesuisvaccine. |
| doi_str_mv | 10.1016/j.vaccine.2007.07.063 |
| format | Article |
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Normal weight gain with no clinical signs was observed in pigs immunized with high doses ofS.C.-Δcrp/vpl-live vaccine. Vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets fromSalmonellainfection. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Furthermore, the peripheral blood mononuclear cells from immunized piglets also developedSalmonellaspecific T-cell proliferative responsein vitro. Our results indicate that immunogenic antigens inS.C.-Δcrp/vpl-can induce adequate immunity to protect pigs against challenge with a heterologous virulent strain. Thus, this mutant holds promise for the development of a new liveS. choleraesuisvaccine.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2007.07.063</identifier><language>eng</language><publisher>Kidlington: Elsevier Limited</publisher><subject>Bacteria ; Carbon sources ; Genes ; Genetic engineering ; Hogs ; Identification systems ; Immunization ; Laboratory animals ; Mortality ; Swine ; Vaccines</subject><ispartof>Vaccine, 2007-10, Vol.25 (41), p.7031</ispartof><rights>Copyright Elsevier Limited Oct 10, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1558789205?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,64362,64366,72216</link.rule.ids></links><search><creatorcontrib>Chu, Chun-Yen</creatorcontrib><creatorcontrib>Wang, Shiang-Yiu</creatorcontrib><creatorcontrib>Chen, Zeng-Weng</creatorcontrib><creatorcontrib>Chien, Maw-Sheng</creatorcontrib><creatorcontrib>Huang, Ji-Ping</creatorcontrib><creatorcontrib>Chen, Jen-Jin</creatorcontrib><creatorcontrib>Hong, Li-Shian</creatorcontrib><creatorcontrib>Shiau, Ai-Li</creatorcontrib><creatorcontrib>Tsai, Jeng-Liang</creatorcontrib><creatorcontrib>Wu, Chao-Liang</creatorcontrib><title>Heterologous protection in pigs induced by a plasmid-cured andcrpgene-deletedSalmonella choleraesuislive vaccine</title><title>Vaccine</title><description>In this study, we exploited acrp(cAMP receptor protein) gene-deleted, virulence plasmid-curedSalmonella choleraesuismutant with decreased carbon source utilization, designatedS.C.-Δcrp/vpl-, as a live vaccine strain. Normal weight gain with no clinical signs was observed in pigs immunized with high doses ofS.C.-Δcrp/vpl-live vaccine. Vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets fromSalmonellainfection. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Furthermore, the peripheral blood mononuclear cells from immunized piglets also developedSalmonellaspecific T-cell proliferative responsein vitro. Our results indicate that immunogenic antigens inS.C.-Δcrp/vpl-can induce adequate immunity to protect pigs against challenge with a heterologous virulent strain. Thus, this mutant holds promise for the development of a new liveS. choleraesuisvaccine.</description><subject>Bacteria</subject><subject>Carbon sources</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Hogs</subject><subject>Identification systems</subject><subject>Immunization</subject><subject>Laboratory 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| source | Elsevier ScienceDirect Journals; ProQuest Central UK/Ireland |
| subjects | Bacteria Carbon sources Genes Genetic engineering Hogs Identification systems Immunization Laboratory animals Mortality Swine Vaccines |
| title | Heterologous protection in pigs induced by a plasmid-cured andcrpgene-deletedSalmonella choleraesuislive vaccine |
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