Mycobacterium tuberculosisarabinomannan-protein conjugates protect against tuberculosis
Lipoarabinomannan (LAM) is a major structural surface component of mycobacteria. Arabinomannan (AM) oligosaccharides derived from LAM ofMycobacterium tuberculosisH37Rv were isolated and covalently conjugated to tetanus toxoid (TT) or to short-term culture filtrate proteins (antigen 85B (Ag85B) or a...
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| Veröffentlicht in: | Vaccine 2003-09, Vol.21 (25-26), p.4081 |
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| container_title | Vaccine |
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| creator | Hamasur, Beston Haile, Melles Pawlowski, Andrzej Schröder, Ulf Williams, Ann Hatch, Graham Hall, Graham Marsh, Philip Källenius, Gunilla Svenson, Stefan B |
| description | Lipoarabinomannan (LAM) is a major structural surface component of mycobacteria. Arabinomannan (AM) oligosaccharides derived from LAM ofMycobacterium tuberculosisH37Rv were isolated and covalently conjugated to tetanus toxoid (TT) or to short-term culture filtrate proteins (antigen 85B (Ag85B) or a 75kDa protein) fromM. tuberculosisstrain Harlingen. The different AM oligosaccharide (AMOs)-protein conjugate vaccine candidates proved to be highly immunogenic, inducing boosterable IgG responses against the AMOs portion of the conjugates in rabbits and guinea-pigs. Proliferation of T-cells from C57BL/6 mice immunized with the conjugates was seen upon in vitro stimulation with PPD. In C57BL/6 mice subcutaneous immunization with the AMOs-antigen 85B conjugate in alum provided significant protection compared to sham (alum only) immunized mice (P |
| doi_str_mv | 10.1016/S0264-410X(03)00274-3 |
| format | Article |
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Arabinomannan (AM) oligosaccharides derived from LAM ofMycobacterium tuberculosisH37Rv were isolated and covalently conjugated to tetanus toxoid (TT) or to short-term culture filtrate proteins (antigen 85B (Ag85B) or a 75kDa protein) fromM. tuberculosisstrain Harlingen. The different AM oligosaccharide (AMOs)-protein conjugate vaccine candidates proved to be highly immunogenic, inducing boosterable IgG responses against the AMOs portion of the conjugates in rabbits and guinea-pigs. Proliferation of T-cells from C57BL/6 mice immunized with the conjugates was seen upon in vitro stimulation with PPD. In C57BL/6 mice subcutaneous immunization with the AMOs-antigen 85B conjugate in alum provided significant protection compared to sham (alum only) immunized mice (P<0.021) as estimated by long term survival against intravenous challenge with 105M. tuberculosisH37Rv. Subcutaneous immunization followed by nasal boost with an AMOs-TT conjugate in Eurocine(TM) L3 adjuvant provided high (P<0.025) protection as determined by long term survival after intranasal challenge with 105virulentM. tuberculosisstrain Harlingen. This level of protection was comparable to that obtained with the conventional live attenuated BCG vaccine. In guinea-pigs, immunization with AMOs-Ag85B in Eurocine(TM) L3 adjuvant followed by aerogenic challenge withM. tuberculosisH37Rv resulted in increased survival and reduced pathology in lungs and spleens relative to non-immunized animals.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(03)00274-3</identifier><language>eng</language><publisher>Kidlington: Elsevier Limited</publisher><subject>Bacteria ; Candidates ; Carbohydrates ; Cell growth ; Chromatography ; Filtrate ; Immunization ; Lipids ; Molecular weight ; Proteins ; Survival ; Tuberculosis ; Vaccines</subject><ispartof>Vaccine, 2003-09, Vol.21 (25-26), p.4081</ispartof><rights>Copyright Elsevier Limited Sep 8, 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1549332242?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,64364,64368,72218</link.rule.ids></links><search><creatorcontrib>Hamasur, Beston</creatorcontrib><creatorcontrib>Haile, Melles</creatorcontrib><creatorcontrib>Pawlowski, Andrzej</creatorcontrib><creatorcontrib>Schröder, Ulf</creatorcontrib><creatorcontrib>Williams, Ann</creatorcontrib><creatorcontrib>Hatch, Graham</creatorcontrib><creatorcontrib>Hall, Graham</creatorcontrib><creatorcontrib>Marsh, Philip</creatorcontrib><creatorcontrib>Källenius, Gunilla</creatorcontrib><creatorcontrib>Svenson, Stefan B</creatorcontrib><title>Mycobacterium tuberculosisarabinomannan-protein conjugates protect against tuberculosis</title><title>Vaccine</title><description>Lipoarabinomannan (LAM) is a major structural surface component of mycobacteria. Arabinomannan (AM) oligosaccharides derived from LAM ofMycobacterium tuberculosisH37Rv were isolated and covalently conjugated to tetanus toxoid (TT) or to short-term culture filtrate proteins (antigen 85B (Ag85B) or a 75kDa protein) fromM. tuberculosisstrain Harlingen. The different AM oligosaccharide (AMOs)-protein conjugate vaccine candidates proved to be highly immunogenic, inducing boosterable IgG responses against the AMOs portion of the conjugates in rabbits and guinea-pigs. Proliferation of T-cells from C57BL/6 mice immunized with the conjugates was seen upon in vitro stimulation with PPD. In C57BL/6 mice subcutaneous immunization with the AMOs-antigen 85B conjugate in alum provided significant protection compared to sham (alum only) immunized mice (P<0.021) as estimated by long term survival against intravenous challenge with 105M. tuberculosisH37Rv. Subcutaneous immunization followed by nasal boost with an AMOs-TT conjugate in Eurocine(TM) L3 adjuvant provided high (P<0.025) protection as determined by long term survival after intranasal challenge with 105virulentM. tuberculosisstrain Harlingen. This level of protection was comparable to that obtained with the conventional live attenuated BCG vaccine. 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| language | eng |
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| source | Elsevier ScienceDirect Journals; ProQuest Central UK/Ireland |
| subjects | Bacteria Candidates Carbohydrates Cell growth Chromatography Filtrate Immunization Lipids Molecular weight Proteins Survival Tuberculosis Vaccines |
| title | Mycobacterium tuberculosisarabinomannan-protein conjugates protect against tuberculosis |
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