Genotoxicity of all-transretinoic acid (ATRA) and its steroidal analogue EA-4 in human lymphocytes and mouse cellsin vitro
The aim of our study is to: (a) investigate whether ATRA and its steroidal analogue EA-4 enhance micronucleation in human lymphocytes and mouse cellsin vitroand clarify the micronucleation mechanism by FISH and CREST analysis respectively, and (b) analyze their effect on spindle organization by immu...
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| Veröffentlicht in: | Cancer letters 2011-07, Vol.306 (1), p.15 |
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| creator | Alakhras, Raghda S Stephanou, Georgia Demopoulos, Nikos A Nikolaropoulos, Sotiris S |
| description | The aim of our study is to: (a) investigate whether ATRA and its steroidal analogue EA-4 enhance micronucleation in human lymphocytes and mouse cellsin vitroand clarify the micronucleation mechanism by FISH and CREST analysis respectively, and (b) analyze their effect on spindle organization by immunofluorescence of β- and γ-tubulin in mouse cells. We found that they: (a) induce micronucleation mainly via chromosome breakage and chromosome delay in a lesser extent, (b) disturb microtubule network, chromosome orientation and centrosome duplication/separation, (c) accumulate cell cycle at ana-telophases, which exert micronucleation, multiple γ-tubulin signals, nucleoplasmic bridges and multinucleation, and (d) generate multinucleated and multimicronucleated interphase cells. |
| doi_str_mv | 10.1016/j.canlet.2011.02.010 |
| format | Article |
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We found that they: (a) induce micronucleation mainly via chromosome breakage and chromosome delay in a lesser extent, (b) disturb microtubule network, chromosome orientation and centrosome duplication/separation, (c) accumulate cell cycle at ana-telophases, which exert micronucleation, multiple γ-tubulin signals, nucleoplasmic bridges and multinucleation, and (d) generate multinucleated and multimicronucleated interphase cells.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2011.02.010</identifier><language>eng</language><publisher>Clare: Elsevier Limited</publisher><subject>Acids ; Cancer ; Cancer therapies ; Cell division ; Chemical compounds ; Chromosomes ; Cytotoxicity ; Gender differences ; Gene expression ; Hybridization ; Lymphocytes ; Rodents ; Vitamin A</subject><ispartof>Cancer letters, 2011-07, Vol.306 (1), p.15</ispartof><rights>Copyright Elsevier Limited Jul 1, 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Alakhras, Raghda S</creatorcontrib><creatorcontrib>Stephanou, Georgia</creatorcontrib><creatorcontrib>Demopoulos, Nikos A</creatorcontrib><creatorcontrib>Nikolaropoulos, Sotiris S</creatorcontrib><title>Genotoxicity of all-transretinoic acid (ATRA) and its steroidal analogue EA-4 in human lymphocytes and mouse cellsin vitro</title><title>Cancer letters</title><description>The aim of our study is to: (a) investigate whether ATRA and its steroidal analogue EA-4 enhance micronucleation in human lymphocytes and mouse cellsin vitroand clarify the micronucleation mechanism by FISH and CREST analysis respectively, and (b) analyze their effect on spindle organization by immunofluorescence of β- and γ-tubulin in mouse cells. We found that they: (a) induce micronucleation mainly via chromosome breakage and chromosome delay in a lesser extent, (b) disturb microtubule network, chromosome orientation and centrosome duplication/separation, (c) accumulate cell cycle at ana-telophases, which exert micronucleation, multiple γ-tubulin signals, nucleoplasmic bridges and multinucleation, and (d) generate multinucleated and multimicronucleated interphase cells.</description><subject>Acids</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Cell division</subject><subject>Chemical compounds</subject><subject>Chromosomes</subject><subject>Cytotoxicity</subject><subject>Gender differences</subject><subject>Gene expression</subject><subject>Hybridization</subject><subject>Lymphocytes</subject><subject>Rodents</subject><subject>Vitamin A</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqNjs1KxDAUhYMoWEffwMUFN7pI56Y_trMsMupaZj-ENOOkpLljbirWp7eID-DqwOH7DkeIW4W5QvW4HnKjg7cpL1CpHIscFZ6JTLVNIZtNi-ciwxIrWbZlfSmumAdErKumzsT3iw2U6MsZl2agA2jvZYo6cLTJBXIGtHE93He7t-4BdOjBJQZONpLrtV8a7el9srDtZAUuwHEadQA_j6cjmTlZ_pVGmtiCsd7zwny6FOlaXBy0Z3vzlytx97zdPb3KU6SPyXLaDzTFZZ73ajlbVsWmrcr_UT80JVYQ</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Alakhras, Raghda S</creator><creator>Stephanou, Georgia</creator><creator>Demopoulos, Nikos A</creator><creator>Nikolaropoulos, Sotiris S</creator><general>Elsevier Limited</general><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope></search><sort><creationdate>20110701</creationdate><title>Genotoxicity of all-transretinoic acid (ATRA) and its steroidal analogue EA-4 in human lymphocytes and mouse cellsin vitro</title><author>Alakhras, Raghda S ; Stephanou, Georgia ; Demopoulos, Nikos A ; Nikolaropoulos, Sotiris S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_15473429843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acids</topic><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Cell division</topic><topic>Chemical compounds</topic><topic>Chromosomes</topic><topic>Cytotoxicity</topic><topic>Gender differences</topic><topic>Gene expression</topic><topic>Hybridization</topic><topic>Lymphocytes</topic><topic>Rodents</topic><topic>Vitamin A</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alakhras, Raghda S</creatorcontrib><creatorcontrib>Stephanou, Georgia</creatorcontrib><creatorcontrib>Demopoulos, Nikos A</creatorcontrib><creatorcontrib>Nikolaropoulos, Sotiris S</creatorcontrib><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alakhras, Raghda S</au><au>Stephanou, Georgia</au><au>Demopoulos, Nikos A</au><au>Nikolaropoulos, Sotiris S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotoxicity of all-transretinoic acid (ATRA) and its steroidal analogue EA-4 in human lymphocytes and mouse cellsin vitro</atitle><jtitle>Cancer letters</jtitle><date>2011-07-01</date><risdate>2011</risdate><volume>306</volume><issue>1</issue><spage>15</spage><pages>15-</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>The aim of our study is to: (a) investigate whether ATRA and its steroidal analogue EA-4 enhance micronucleation in human lymphocytes and mouse cellsin vitroand clarify the micronucleation mechanism by FISH and CREST analysis respectively, and (b) analyze their effect on spindle organization by immunofluorescence of β- and γ-tubulin in mouse cells. We found that they: (a) induce micronucleation mainly via chromosome breakage and chromosome delay in a lesser extent, (b) disturb microtubule network, chromosome orientation and centrosome duplication/separation, (c) accumulate cell cycle at ana-telophases, which exert micronucleation, multiple γ-tubulin signals, nucleoplasmic bridges and multinucleation, and (d) generate multinucleated and multimicronucleated interphase cells.</abstract><cop>Clare</cop><pub>Elsevier Limited</pub><doi>10.1016/j.canlet.2011.02.010</doi></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Acids Cancer Cancer therapies Cell division Chemical compounds Chromosomes Cytotoxicity Gender differences Gene expression Hybridization Lymphocytes Rodents Vitamin A |
| title | Genotoxicity of all-transretinoic acid (ATRA) and its steroidal analogue EA-4 in human lymphocytes and mouse cellsin vitro |
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