Radiolabeled RGD Tracer Kinetics Annotates Differential [alpha]^sub v^[beta]^sub 3^ Integrin Expression Linked to Cell Intrinsic and Vessel Expression

Radiolabeled RGD Tracer Kinetics Annotates Differential [alpha]^sub v^[beta]^sub 3^ Integrin Expression Linked to Cell Intrinsic and Vessel Expression

The purpose of this paper is to study the association between RGD binding kinetics and [alpha]^sub v^[beta]^sub 3^ integrin receptor density in the complex tumor milieu. We assessed [alpha]^sub v^[beta]^sub 3^ in vitro and by ^sup 68^Ga-DOTA-[c(RGDfK)]^sub 2^ positron emission tomography (PET) in tu...

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Veröffentlicht in:Molecular imaging and biology 2014-08, Vol.16 (4), p.558
Hauptverfasser: Alam, Israt S, Witney, Timothy H, Tomasi, Giampaolo, Carroll, Laurence, Twyman, Frazer J, Nguyen, Quang-dé, Aboagye, Eric O
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container_title Molecular imaging and biology
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creator Alam, Israt S
Witney, Timothy H
Tomasi, Giampaolo
Carroll, Laurence
Twyman, Frazer J
Nguyen, Quang-dé
Aboagye, Eric O
description The purpose of this paper is to study the association between RGD binding kinetics and [alpha]^sub v^[beta]^sub 3^ integrin receptor density in the complex tumor milieu. We assessed [alpha]^sub v^[beta]^sub 3^ in vitro and by ^sup 68^Ga-DOTA-[c(RGDfK)]^sub 2^ positron emission tomography (PET) in tumors with varying [alpha]^sub v^[beta]^sub 3^. Intrinsic [alpha]^sub v^[beta]^sub 3^ expression decreased in the order of M21>>>MDA-MB-231>M21L in cells. Tumor volume of distribution by PET, V ^sub T^, was significantly higher in M21 compared to isogenic M21L tumors (0.40±0.01 versus 0.25±0.02; p
doi_str_mv 10.1007/s11307-013-0710-3
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We assessed [alpha]^sub v^[beta]^sub 3^ in vitro and by ^sup 68^Ga-DOTA-[c(RGDfK)]^sub 2^ positron emission tomography (PET) in tumors with varying [alpha]^sub v^[beta]^sub 3^. Intrinsic [alpha]^sub v^[beta]^sub 3^ expression decreased in the order of M21&gt;&gt;&gt;MDA-MB-231&gt;M21L in cells. Tumor volume of distribution by PET, V ^sub T^, was significantly higher in M21 compared to isogenic M21L tumors (0.40±0.01 versus 0.25±0.02; p&lt;0.01) despite similar microvessel density (MVD) likely due to higher [alpha]^sub v^[beta]^sub 3^. V ^sub T^ for MDA-MB-231 (0.40±0.04) was comparable to M21 despite lower [alpha]^sub v^[beta]^sub 3^ but in keeping with the higher MVD, suggesting superior tracer distribution. 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title Radiolabeled RGD Tracer Kinetics Annotates Differential [alpha]^sub v^[beta]^sub 3^ Integrin Expression Linked to Cell Intrinsic and Vessel Expression
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