Evaluation of High-resolution Melting Curve Analysis as a New Tool for Root-knot Nematode Diagnostics
In the detection of plant pests, speed and accuracy are vital. High‐resolution melting curve (HRMC) analysis was therefore evaluated as a new tool for the identification of root‐knot nematodes (Meloidogyne spp.). On the basis of the second intergenic spacer (IGS2) region of the ribosomal DNA cistron...
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| Veröffentlicht in: | Journal of phytopathology 2012-02, Vol.160 (2), p.59-66 |
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| creator | Holterman, Martijn H. M. Oggenfuss, Markus Frey, Juerg Ernst Kiewnick, Sebastian |
| description | In the detection of plant pests, speed and accuracy are vital. High‐resolution melting curve (HRMC) analysis was therefore evaluated as a new tool for the identification of root‐knot nematodes (Meloidogyne spp.). On the basis of the second intergenic spacer (IGS2) region of the ribosomal DNA cistron, Meloidogyne chitwoodi, M. fallax and M. hapla were successfully distinguished from each other and the group of the three tropical species, M. incognita, M. arenaria and M. javanica. Conversely, it was shown that the IGS2 region is not suitable for the tropical species M. enterolobii (senior synonym of M. mayaguensis) as the amplification of multiple fragments of different lengths prevented a reliable HRMC analysis. However, the obtained results provide a proof of principle that HRMC analysis can be a suitable single‐tube assay for fast and accurate root‐knot nematode identification. |
| doi_str_mv | 10.1111/j.1439-0434.2011.01859.x |
| format | Article |
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Conversely, it was shown that the IGS2 region is not suitable for the tropical species M. enterolobii (senior synonym of M. mayaguensis) as the amplification of multiple fragments of different lengths prevented a reliable HRMC analysis. However, the obtained results provide a proof of principle that HRMC analysis can be a suitable single‐tube assay for fast and accurate root‐knot nematode identification.</description><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>high-resolution melting curve analysis</subject><subject>IGS2</subject><subject>Meloidogyne spp</subject><subject>molecular diagnostics</subject><subject>Phytopathology. Animal pests. 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M.</creatorcontrib><creatorcontrib>Oggenfuss, Markus</creatorcontrib><creatorcontrib>Frey, Juerg Ernst</creatorcontrib><creatorcontrib>Kiewnick, Sebastian</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holterman, Martijn H. 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| subjects | Biological and medical sciences Fundamental and applied biological sciences. Psychology high-resolution melting curve analysis IGS2 Meloidogyne spp molecular diagnostics Phytopathology. Animal pests. Plant and forest protection ribosomal DNA root-knot nematode species identification |
| title | Evaluation of High-resolution Melting Curve Analysis as a New Tool for Root-knot Nematode Diagnostics |
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