Efficient [Phi]C31 integrase-mediated site-specific germline transformation of Anopheles gambiae

Current transgenic methodology developed for mosquitoes has not been applied widely to the major malaria vector Anopheles gambiae, which has proved more difficult to genetically manipulate than other mosquito species and dipteran insects. In this protocol, we describe ΦC31-mediated site-specific int...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Nature protocols 2014-07, Vol.9 (7), p.1698
Hauptverfasser:	Pondeville, Emilie, Puchot, Nicolas, Meredith, Janet M, Lynd, Amy, Vernick, Kenneth D, Lycett, Gareth J, Eggleston, Paul, Bourgouin, Catherine
Format:	Artikel
Sprache:	eng
Schlagworte:	Aquatic insects Embryos Gene expression Genetic engineering Genomes Injection Insects Larvae Malaria Mosquitoes Parasites Protocol Vector-borne diseases
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!