Platelet-derived growth factor-BB induces matrix metalloproteinase-2 expression and rat vascular smooth muscle cell migration via ROCK and ERK/p38 MAPK pathways
Matrix metalloproteinases (MMP) play a pivotal role in the pathogenesis of cardiovascular diseases. Their expressions are altered in response to a variety of stimuli, including growth factors, inflammatory markers, and cytokines. In this study, we demonstrated that platelet-derived growth factor-BB...
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| Veröffentlicht in: | Molecular and cellular biochemistry 2014-08, Vol.393 (1-2), p.255-263 |
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| creator | Cui, Ying Sun, Yin-Wei Lin, Hai-Shuang Su, Wei-Min Fang, Yan Zhao, Ying Wei, Xiao-Qing Qin, Yuan-Hua Kohama, Kazuhiro Gao, Ying |
| description | Matrix metalloproteinases (MMP) play a pivotal role in the pathogenesis of cardiovascular diseases. Their expressions are altered in response to a variety of stimuli, including growth factors, inflammatory markers, and cytokines. In this study, we demonstrated that platelet-derived growth factor-BB (PDGF-BB) induces a dose- and time-dependent increase in MMP-2 expression in rat vascular smooth muscle cells (VSMC). Treatment with either the Rho-associated protein kinase (ROCK) inhibitor Y-27632 or suppression of ROCK-1/2 by small interfering RNA technology significantly reduced the MMP-2 expression, thus suggesting that ROCK regulates such expression. Similar results were observed when VSMC were pretreated with either U0126 or SB203580, which are selective inhibitors of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase, respectively, thus suggesting that these kinases are important for the induction of MMP-2 expression by PDGF-BB. In conclusion, these results described a novel mechanism in atherosclerosis through PDGF-BB signaling in VSMC, in which MMP-2 expression is induced via extracellular signal-regulated kinases and p38 mitogen-activated protein kinase phosphorylation, as well as ROCK. |
| doi_str_mv | 10.1007/s11010-014-2068-5 |
| format | Article |
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Their expressions are altered in response to a variety of stimuli, including growth factors, inflammatory markers, and cytokines. In this study, we demonstrated that platelet-derived growth factor-BB (PDGF-BB) induces a dose- and time-dependent increase in MMP-2 expression in rat vascular smooth muscle cells (VSMC). Treatment with either the Rho-associated protein kinase (ROCK) inhibitor Y-27632 or suppression of ROCK-1/2 by small interfering RNA technology significantly reduced the MMP-2 expression, thus suggesting that ROCK regulates such expression. Similar results were observed when VSMC were pretreated with either U0126 or SB203580, which are selective inhibitors of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase, respectively, thus suggesting that these kinases are important for the induction of MMP-2 expression by PDGF-BB. In conclusion, these results described a novel mechanism in atherosclerosis through PDGF-BB signaling in VSMC, in which MMP-2 expression is induced via extracellular signal-regulated kinases and p38 mitogen-activated protein kinase phosphorylation, as well as ROCK.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-014-2068-5</identifier><identifier>PMID: 24792035</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject><![CDATA[Amides - administration & dosage ; Animals ; Aorta - cytology ; Aorta - metabolism ; Atherosclerosis ; Atherosclerosis - etiology ; Atherosclerosis - metabolism ; Atherosclerosis - pathology ; Biochemistry ; Biomedical and Life Sciences ; Butadienes - administration & dosage ; Cardiology ; Cardiovascular diseases ; Cell adhesion & migration ; Cell Line ; Cell Movement - drug effects ; Cytokines ; Gene Expression Regulation - drug effects ; Growth factors ; Humans ; Imidazoles - administration & dosage ; Life Sciences ; Matrix Metalloproteinase 2 - biosynthesis ; Matrix Metalloproteinase 2 - metabolism ; Medical Biochemistry ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - metabolism ; Nitriles - administration & dosage ; Oncology ; p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors ; p38 Mitogen-Activated Protein Kinases - metabolism ; Phosphotransferases ; Platelet-derived growth factor ; Protein expression ; Proto-Oncogene Proteins c-sis - administration & dosage ; Proto-Oncogene Proteins c-sis - metabolism ; Pyridines - administration & dosage ; Rats ; rho-Associated Kinases - antagonists & inhibitors ; rho-Associated Kinases - metabolism ; RNA ; Rocks ; Rodents ; Signal transduction ; Smooth muscle]]></subject><ispartof>Molecular and cellular biochemistry, 2014-08, Vol.393 (1-2), p.255-263</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>COPYRIGHT 2014 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-35af43dd4eee3fe8fafc5bb15004f3d0b0c49c27675725dccadd8ae5cee60c4d3</citedby><cites>FETCH-LOGICAL-c538t-35af43dd4eee3fe8fafc5bb15004f3d0b0c49c27675725dccadd8ae5cee60c4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11010-014-2068-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11010-014-2068-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24792035$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cui, Ying</creatorcontrib><creatorcontrib>Sun, Yin-Wei</creatorcontrib><creatorcontrib>Lin, Hai-Shuang</creatorcontrib><creatorcontrib>Su, Wei-Min</creatorcontrib><creatorcontrib>Fang, Yan</creatorcontrib><creatorcontrib>Zhao, Ying</creatorcontrib><creatorcontrib>Wei, Xiao-Qing</creatorcontrib><creatorcontrib>Qin, Yuan-Hua</creatorcontrib><creatorcontrib>Kohama, Kazuhiro</creatorcontrib><creatorcontrib>Gao, Ying</creatorcontrib><title>Platelet-derived growth factor-BB induces matrix metalloproteinase-2 expression and rat vascular smooth muscle cell migration via ROCK and ERK/p38 MAPK pathways</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><addtitle>Mol Cell Biochem</addtitle><description>Matrix metalloproteinases (MMP) play a pivotal role in the pathogenesis of cardiovascular diseases. Their expressions are altered in response to a variety of stimuli, including growth factors, inflammatory markers, and cytokines. In this study, we demonstrated that platelet-derived growth factor-BB (PDGF-BB) induces a dose- and time-dependent increase in MMP-2 expression in rat vascular smooth muscle cells (VSMC). Treatment with either the Rho-associated protein kinase (ROCK) inhibitor Y-27632 or suppression of ROCK-1/2 by small interfering RNA technology significantly reduced the MMP-2 expression, thus suggesting that ROCK regulates such expression. Similar results were observed when VSMC were pretreated with either U0126 or SB203580, which are selective inhibitors of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase, respectively, thus suggesting that these kinases are important for the induction of MMP-2 expression by PDGF-BB. In conclusion, these results described a novel mechanism in atherosclerosis through PDGF-BB signaling in VSMC, in which MMP-2 expression is induced via extracellular signal-regulated kinases and p38 mitogen-activated protein kinase phosphorylation, as well as ROCK.</description><subject>Amides - administration & dosage</subject><subject>Animals</subject><subject>Aorta - cytology</subject><subject>Aorta - metabolism</subject><subject>Atherosclerosis</subject><subject>Atherosclerosis - etiology</subject><subject>Atherosclerosis - metabolism</subject><subject>Atherosclerosis - pathology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Butadienes - administration & dosage</subject><subject>Cardiology</subject><subject>Cardiovascular diseases</subject><subject>Cell adhesion & migration</subject><subject>Cell Line</subject><subject>Cell Movement - drug effects</subject><subject>Cytokines</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Imidazoles - administration & dosage</subject><subject>Life Sciences</subject><subject>Matrix Metalloproteinase 2 - biosynthesis</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Medical Biochemistry</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Nitriles - administration & dosage</subject><subject>Oncology</subject><subject>p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors</subject><subject>p38 Mitogen-Activated Protein Kinases - metabolism</subject><subject>Phosphotransferases</subject><subject>Platelet-derived growth factor</subject><subject>Protein expression</subject><subject>Proto-Oncogene Proteins c-sis - administration & dosage</subject><subject>Proto-Oncogene Proteins c-sis - metabolism</subject><subject>Pyridines - administration & dosage</subject><subject>Rats</subject><subject>rho-Associated Kinases - antagonists & inhibitors</subject><subject>rho-Associated Kinases - metabolism</subject><subject>RNA</subject><subject>Rocks</subject><subject>Rodents</subject><subject>Signal transduction</subject><subject>Smooth 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growth factor-BB induces matrix metalloproteinase-2 expression and rat vascular smooth muscle cell migration via ROCK and ERK/p38 MAPK pathways</title><author>Cui, Ying ; Sun, Yin-Wei ; Lin, Hai-Shuang ; Su, Wei-Min ; Fang, Yan ; Zhao, Ying ; Wei, Xiao-Qing ; Qin, Yuan-Hua ; Kohama, Kazuhiro ; Gao, Ying</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-35af43dd4eee3fe8fafc5bb15004f3d0b0c49c27675725dccadd8ae5cee60c4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Amides - administration & dosage</topic><topic>Animals</topic><topic>Aorta - cytology</topic><topic>Aorta - metabolism</topic><topic>Atherosclerosis</topic><topic>Atherosclerosis - etiology</topic><topic>Atherosclerosis - metabolism</topic><topic>Atherosclerosis - pathology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Butadienes - administration & dosage</topic><topic>Cardiology</topic><topic>Cardiovascular diseases</topic><topic>Cell adhesion & migration</topic><topic>Cell Line</topic><topic>Cell Movement - drug effects</topic><topic>Cytokines</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Imidazoles - administration & dosage</topic><topic>Life Sciences</topic><topic>Matrix Metalloproteinase 2 - biosynthesis</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Medical Biochemistry</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Nitriles - administration & dosage</topic><topic>Oncology</topic><topic>p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>Phosphotransferases</topic><topic>Platelet-derived growth factor</topic><topic>Protein expression</topic><topic>Proto-Oncogene 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Biochem</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>393</volume><issue>1-2</issue><spage>255</spage><epage>263</epage><pages>255-263</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Matrix metalloproteinases (MMP) play a pivotal role in the pathogenesis of cardiovascular diseases. Their expressions are altered in response to a variety of stimuli, including growth factors, inflammatory markers, and cytokines. In this study, we demonstrated that platelet-derived growth factor-BB (PDGF-BB) induces a dose- and time-dependent increase in MMP-2 expression in rat vascular smooth muscle cells (VSMC). Treatment with either the Rho-associated protein kinase (ROCK) inhibitor Y-27632 or suppression of ROCK-1/2 by small interfering RNA technology significantly reduced the MMP-2 expression, thus suggesting that ROCK regulates such expression. Similar results were observed when VSMC were pretreated with either U0126 or SB203580, which are selective inhibitors of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase, respectively, thus suggesting that these kinases are important for the induction of MMP-2 expression by PDGF-BB. In conclusion, these results described a novel mechanism in atherosclerosis through PDGF-BB signaling in VSMC, in which MMP-2 expression is induced via extracellular signal-regulated kinases and p38 mitogen-activated protein kinase phosphorylation, as well as ROCK.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>24792035</pmid><doi>10.1007/s11010-014-2068-5</doi><tpages>9</tpages></addata></record> |
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| ispartof | Molecular and cellular biochemistry, 2014-08, Vol.393 (1-2), p.255-263 |
| issn | 0300-8177 1573-4919 |
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| subjects | Amides - administration & dosage Animals Aorta - cytology Aorta - metabolism Atherosclerosis Atherosclerosis - etiology Atherosclerosis - metabolism Atherosclerosis - pathology Biochemistry Biomedical and Life Sciences Butadienes - administration & dosage Cardiology Cardiovascular diseases Cell adhesion & migration Cell Line Cell Movement - drug effects Cytokines Gene Expression Regulation - drug effects Growth factors Humans Imidazoles - administration & dosage Life Sciences Matrix Metalloproteinase 2 - biosynthesis Matrix Metalloproteinase 2 - metabolism Medical Biochemistry Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Nitriles - administration & dosage Oncology p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors p38 Mitogen-Activated Protein Kinases - metabolism Phosphotransferases Platelet-derived growth factor Protein expression Proto-Oncogene Proteins c-sis - administration & dosage Proto-Oncogene Proteins c-sis - metabolism Pyridines - administration & dosage Rats rho-Associated Kinases - antagonists & inhibitors rho-Associated Kinases - metabolism RNA Rocks Rodents Signal transduction Smooth muscle |
| title | Platelet-derived growth factor-BB induces matrix metalloproteinase-2 expression and rat vascular smooth muscle cell migration via ROCK and ERK/p38 MAPK pathways |
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