VvMATE1 and VvMATE2 encode putative proanthocyanidin transporters expressed during berry development in Vitis vinifera L
KEY MESSAGE : VvMATE1 and VvMATE2 encode putative PA transporters expressed during seed development in grapevine. The subcellular localization of these MATE proteins suggests different routes for the intracellular transport of PAs. Proanthocyanidins (PAs), also called condensed tannins, protect plan...
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| Veröffentlicht in: | Plant cell reports 2014-07, Vol.33 (7), p.1147-1159 |
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| creator | Pérez-Díaz, Ricardo Ryngajllo, Malgorzata Pérez-Díaz, Jorge Peña-Cortés, Hugo Casaretto, José A González-Villanueva, Enrique Ruiz-Lara, Simón |
| description | KEY MESSAGE : VvMATE1 and VvMATE2 encode putative PA transporters expressed during seed development in grapevine. The subcellular localization of these MATE proteins suggests different routes for the intracellular transport of PAs. Proanthocyanidins (PAs), also called condensed tannins, protect plants against herbivores and are important quality components of many fruits. PAs biosynthesis is part of the flavonoid pathway that also produces anthocyanins and flavonols. In grape fruits, PAs are present in seeds and skin tissues. PAs are synthesized in the cytoplasm and accumulated into the vacuole and apoplast; however, little is known about the mechanisms involved in the transport of these compounds to such cellular compartments. A gene encoding a Multidrug And Toxic compound Extrusion (MATE) family protein suggested to transport anthocyanins—named VvMATE1—was used to identify a second gene of the MATE family, VvMATE2. Analysis of their deduced amino acid sequences and the phylogenetic relationship with other MATE-like proteins indicated that VvMATE1 and VvMATE2 encode putative PA transporters. Subcellular localization assays in Arabidopsis protoplasts transformed with VvMATE–GFP fusion constructs along with organelle-specific markers revealed that VvMATE1 is localized in the tonoplast whereas VvMATE2 is localized in the Golgi complex. Major expression of both genes occurs during the early stages of seed development concomitant with the accumulation of PAs. Both genes are poorly expressed in the skin of berries while VvMATE2 is also expressed in leaves. The presence of putative cis-acting elements in the promoters of VvMATE1 and VvMATE2 may explain the differential transcriptional regulation of these genes in grapevine. Altogether, these results suggest that these MATE proteins could mediate the transport and accumulation of PAs in grapevine through different routes and cellular compartments. |
| doi_str_mv | 10.1007/s00299-014-1604-9 |
| format | Article |
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The subcellular localization of these MATE proteins suggests different routes for the intracellular transport of PAs. Proanthocyanidins (PAs), also called condensed tannins, protect plants against herbivores and are important quality components of many fruits. PAs biosynthesis is part of the flavonoid pathway that also produces anthocyanins and flavonols. In grape fruits, PAs are present in seeds and skin tissues. PAs are synthesized in the cytoplasm and accumulated into the vacuole and apoplast; however, little is known about the mechanisms involved in the transport of these compounds to such cellular compartments. A gene encoding a Multidrug And Toxic compound Extrusion (MATE) family protein suggested to transport anthocyanins—named VvMATE1—was used to identify a second gene of the MATE family, VvMATE2. Analysis of their deduced amino acid sequences and the phylogenetic relationship with other MATE-like proteins indicated that VvMATE1 and VvMATE2 encode putative PA transporters. Subcellular localization assays in Arabidopsis protoplasts transformed with VvMATE–GFP fusion constructs along with organelle-specific markers revealed that VvMATE1 is localized in the tonoplast whereas VvMATE2 is localized in the Golgi complex. Major expression of both genes occurs during the early stages of seed development concomitant with the accumulation of PAs. Both genes are poorly expressed in the skin of berries while VvMATE2 is also expressed in leaves. The presence of putative cis-acting elements in the promoters of VvMATE1 and VvMATE2 may explain the differential transcriptional regulation of these genes in grapevine. Altogether, these results suggest that these MATE proteins could mediate the transport and accumulation of PAs in grapevine through different routes and cellular compartments.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-014-1604-9</identifier><identifier>PMID: 24700246</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Amino acids ; anthocyanins ; apoplast ; Arabidopsis ; Arabidopsis - genetics ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cell Biology ; extrusion ; flavonols ; Fruit - genetics ; Fruit - growth & development ; Fruits ; Gene Expression Regulation, Plant ; genes ; Golgi apparatus ; Golgi Apparatus - metabolism ; grapes ; Herbivores ; leaves ; Life Sciences ; Molecular Sequence Data ; Original Paper ; Phylogeny ; Plant Biochemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Sciences ; proanthocyanidins ; Proanthocyanidins - metabolism ; protoplasts ; seed development ; seeds ; Seeds - genetics ; Seeds - growth & development ; Sequence Homology, Amino Acid ; small fruits ; transcription (genetics) ; transporters ; vacuoles ; Vitis - genetics ; Vitis - growth & development ; Vitis vinifera</subject><ispartof>Plant cell reports, 2014-07, Vol.33 (7), p.1147-1159</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-f85f07040294007c490d8e377f1600e716f212651ac8e21499c66e09c260d89e3</citedby><cites>FETCH-LOGICAL-c462t-f85f07040294007c490d8e377f1600e716f212651ac8e21499c66e09c260d89e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-014-1604-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-014-1604-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24700246$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pérez-Díaz, Ricardo</creatorcontrib><creatorcontrib>Ryngajllo, Malgorzata</creatorcontrib><creatorcontrib>Pérez-Díaz, Jorge</creatorcontrib><creatorcontrib>Peña-Cortés, Hugo</creatorcontrib><creatorcontrib>Casaretto, José A</creatorcontrib><creatorcontrib>González-Villanueva, Enrique</creatorcontrib><creatorcontrib>Ruiz-Lara, Simón</creatorcontrib><title>VvMATE1 and VvMATE2 encode putative proanthocyanidin transporters expressed during berry development in Vitis vinifera L</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>KEY MESSAGE : VvMATE1 and VvMATE2 encode putative PA transporters expressed during seed development in grapevine. The subcellular localization of these MATE proteins suggests different routes for the intracellular transport of PAs. Proanthocyanidins (PAs), also called condensed tannins, protect plants against herbivores and are important quality components of many fruits. PAs biosynthesis is part of the flavonoid pathway that also produces anthocyanins and flavonols. In grape fruits, PAs are present in seeds and skin tissues. PAs are synthesized in the cytoplasm and accumulated into the vacuole and apoplast; however, little is known about the mechanisms involved in the transport of these compounds to such cellular compartments. A gene encoding a Multidrug And Toxic compound Extrusion (MATE) family protein suggested to transport anthocyanins—named VvMATE1—was used to identify a second gene of the MATE family, VvMATE2. Analysis of their deduced amino acid sequences and the phylogenetic relationship with other MATE-like proteins indicated that VvMATE1 and VvMATE2 encode putative PA transporters. Subcellular localization assays in Arabidopsis protoplasts transformed with VvMATE–GFP fusion constructs along with organelle-specific markers revealed that VvMATE1 is localized in the tonoplast whereas VvMATE2 is localized in the Golgi complex. Major expression of both genes occurs during the early stages of seed development concomitant with the accumulation of PAs. Both genes are poorly expressed in the skin of berries while VvMATE2 is also expressed in leaves. The presence of putative cis-acting elements in the promoters of VvMATE1 and VvMATE2 may explain the differential transcriptional regulation of these genes in grapevine. Altogether, these results suggest that these MATE proteins could mediate the transport and accumulation of PAs in grapevine through different routes and cellular compartments.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Amino acids</subject><subject>anthocyanins</subject><subject>apoplast</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Biology</subject><subject>extrusion</subject><subject>flavonols</subject><subject>Fruit - genetics</subject><subject>Fruit - growth & development</subject><subject>Fruits</subject><subject>Gene Expression Regulation, Plant</subject><subject>genes</subject><subject>Golgi apparatus</subject><subject>Golgi Apparatus - metabolism</subject><subject>grapes</subject><subject>Herbivores</subject><subject>leaves</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>Original Paper</subject><subject>Phylogeny</subject><subject>Plant Biochemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Sciences</subject><subject>proanthocyanidins</subject><subject>Proanthocyanidins - metabolism</subject><subject>protoplasts</subject><subject>seed development</subject><subject>seeds</subject><subject>Seeds - genetics</subject><subject>Seeds - growth & development</subject><subject>Sequence Homology, Amino Acid</subject><subject>small fruits</subject><subject>transcription (genetics)</subject><subject>transporters</subject><subject>vacuoles</subject><subject>Vitis - genetics</subject><subject>Vitis - growth & development</subject><subject>Vitis vinifera</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1P3DAQxS1UVLbQP4ALWOo5Zew49vqIEP2QFnHgQ9wsk0yoEeuktrNi_3tmFVr11JPn8Htv_N4wdizgqwAwZxlAWluBUJXQoCq7xxZC1bKSUD98YAswUlTGCHXAPuX8DATWRn9kB1IZkiq9YK_3m6vz20vBfez4PEuOsR065ONUfAkbGtLgY_k1tFsfQxciL8nHPA6pYMocX8eEOWPHuymF-MQfMaUt73CDL8O4xlg4Se5DCZlvQgw9Js9XR2y_9y8ZP7-_h-zu2-XtxY9qdf3958X5qmqVlqXql00PBhQFVRS5VRa6JdbG9JQY0AjdSyF1I3y7RCmUta3WCLaVmkCL9SH7MvtSiN8T5uKehylFWulEUzfCNgI0UWKm2jTknLB3Ywprn7ZOgNt17eauHVXodl07S5qTd-fpcY3dX8WfcgmQM5DHXS-Y_ln9H9fTWdT7wfmnFLK7u5EE0PWAfgr1G8eokj4</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Pérez-Díaz, Ricardo</creator><creator>Ryngajllo, Malgorzata</creator><creator>Pérez-Díaz, Jorge</creator><creator>Peña-Cortés, Hugo</creator><creator>Casaretto, José A</creator><creator>González-Villanueva, Enrique</creator><creator>Ruiz-Lara, Simón</creator><general>Springer-Verlag</general><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>20140701</creationdate><title>VvMATE1 and VvMATE2 encode putative proanthocyanidin transporters expressed during berry development in Vitis vinifera L</title><author>Pérez-Díaz, Ricardo ; Ryngajllo, Malgorzata ; Pérez-Díaz, Jorge ; Peña-Cortés, Hugo ; Casaretto, José A ; González-Villanueva, Enrique ; Ruiz-Lara, Simón</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-f85f07040294007c490d8e377f1600e716f212651ac8e21499c66e09c260d89e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Amino acids</topic><topic>anthocyanins</topic><topic>apoplast</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Biology</topic><topic>extrusion</topic><topic>flavonols</topic><topic>Fruit - genetics</topic><topic>Fruit - growth & development</topic><topic>Fruits</topic><topic>Gene Expression Regulation, Plant</topic><topic>genes</topic><topic>Golgi apparatus</topic><topic>Golgi Apparatus - metabolism</topic><topic>grapes</topic><topic>Herbivores</topic><topic>leaves</topic><topic>Life Sciences</topic><topic>Molecular Sequence Data</topic><topic>Original Paper</topic><topic>Phylogeny</topic><topic>Plant Biochemistry</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Sciences</topic><topic>proanthocyanidins</topic><topic>Proanthocyanidins - metabolism</topic><topic>protoplasts</topic><topic>seed development</topic><topic>seeds</topic><topic>Seeds - genetics</topic><topic>Seeds - growth & development</topic><topic>Sequence Homology, Amino Acid</topic><topic>small fruits</topic><topic>transcription (genetics)</topic><topic>transporters</topic><topic>vacuoles</topic><topic>Vitis - genetics</topic><topic>Vitis - growth & development</topic><topic>Vitis vinifera</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pérez-Díaz, Ricardo</creatorcontrib><creatorcontrib>Ryngajllo, Malgorzata</creatorcontrib><creatorcontrib>Pérez-Díaz, Jorge</creatorcontrib><creatorcontrib>Peña-Cortés, Hugo</creatorcontrib><creatorcontrib>Casaretto, José A</creatorcontrib><creatorcontrib>González-Villanueva, Enrique</creatorcontrib><creatorcontrib>Ruiz-Lara, Simón</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pérez-Díaz, Ricardo</au><au>Ryngajllo, Malgorzata</au><au>Pérez-Díaz, Jorge</au><au>Peña-Cortés, Hugo</au><au>Casaretto, José A</au><au>González-Villanueva, Enrique</au><au>Ruiz-Lara, Simón</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>VvMATE1 and VvMATE2 encode putative proanthocyanidin transporters expressed during berry development in Vitis vinifera L</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>33</volume><issue>7</issue><spage>1147</spage><epage>1159</epage><pages>1147-1159</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>KEY MESSAGE : VvMATE1 and VvMATE2 encode putative PA transporters expressed during seed development in grapevine. The subcellular localization of these MATE proteins suggests different routes for the intracellular transport of PAs. Proanthocyanidins (PAs), also called condensed tannins, protect plants against herbivores and are important quality components of many fruits. PAs biosynthesis is part of the flavonoid pathway that also produces anthocyanins and flavonols. In grape fruits, PAs are present in seeds and skin tissues. PAs are synthesized in the cytoplasm and accumulated into the vacuole and apoplast; however, little is known about the mechanisms involved in the transport of these compounds to such cellular compartments. A gene encoding a Multidrug And Toxic compound Extrusion (MATE) family protein suggested to transport anthocyanins—named VvMATE1—was used to identify a second gene of the MATE family, VvMATE2. Analysis of their deduced amino acid sequences and the phylogenetic relationship with other MATE-like proteins indicated that VvMATE1 and VvMATE2 encode putative PA transporters. Subcellular localization assays in Arabidopsis protoplasts transformed with VvMATE–GFP fusion constructs along with organelle-specific markers revealed that VvMATE1 is localized in the tonoplast whereas VvMATE2 is localized in the Golgi complex. Major expression of both genes occurs during the early stages of seed development concomitant with the accumulation of PAs. Both genes are poorly expressed in the skin of berries while VvMATE2 is also expressed in leaves. The presence of putative cis-acting elements in the promoters of VvMATE1 and VvMATE2 may explain the differential transcriptional regulation of these genes in grapevine. Altogether, these results suggest that these MATE proteins could mediate the transport and accumulation of PAs in grapevine through different routes and cellular compartments.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>24700246</pmid><doi>10.1007/s00299-014-1604-9</doi><tpages>13</tpages></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences Amino acids anthocyanins apoplast Arabidopsis Arabidopsis - genetics Biomedical and Life Sciences Biosynthesis Biotechnology Carrier Proteins - genetics Carrier Proteins - metabolism Cell Biology extrusion flavonols Fruit - genetics Fruit - growth & development Fruits Gene Expression Regulation, Plant genes Golgi apparatus Golgi Apparatus - metabolism grapes Herbivores leaves Life Sciences Molecular Sequence Data Original Paper Phylogeny Plant Biochemistry Plant Proteins - genetics Plant Proteins - metabolism Plant Sciences proanthocyanidins Proanthocyanidins - metabolism protoplasts seed development seeds Seeds - genetics Seeds - growth & development Sequence Homology, Amino Acid small fruits transcription (genetics) transporters vacuoles Vitis - genetics Vitis - growth & development Vitis vinifera |
| title | VvMATE1 and VvMATE2 encode putative proanthocyanidin transporters expressed during berry development in Vitis vinifera L |
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