The Toxic Effect of Lithium Ion on Neurons (PC12 cells) and A[beta]^sub 42^ Molecules
In this study, the neurotoxicity of Li ion and its effect on the morphologies of A[beta]^sub 42^ molecules were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, fluorescence (FL), atomic force microscopy (AFM), and circular dichroism (CD) spectroscopy. MTT assa...
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| Veröffentlicht in: | Biological trace element research 2014-06, Vol.159 (1-3), p.410 |
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| creator | Yang, Wang Yan, Zhang Hongjing, Zhang Dexiang, Liu Zengxun, Liu Xiaojing, Cheng Yi, Lu Jichao, Jia Jintong, Liu Fang, Pan |
| description | In this study, the neurotoxicity of Li ion and its effect on the morphologies of A[beta]^sub 42^ molecules were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, fluorescence (FL), atomic force microscopy (AFM), and circular dichroism (CD) spectroscopy. MTT assays show that Li ion with a dosage level lower than 50 mg/l did not show detectable cytotoxicity on pheochromocytoma (PC12) cells whereas a dosage level higher than 100 mg/l resulted in significant cytotoxicity. The interaction between A[beta]^sub 42^ and Li ion occurs, and the quenching effect of Li ion on the fluorescence emission of A[Beta]^sub 42^ is found to be concentration dependent, suggesting that Li ion can bind to the A[beta]^sub 42^ molecules. CD results suggest that a more incompact conformation state will be adopted upon the interaction between A[beta]^sub 42^ and Li ion. According to AFM images, Li ion could induce the formation of the fibrils after incubation for 3 or 5 days. The formation of the oligomer and fibrils originates from the strong interactions between A[beta]^sub 42^ and Li ion. Li ion could accelerate the random coil A[beta]^sub 42^ monomers aggregating into the [beta]-sheet fibrils, which would induce the neurotoxic effect.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1007/s12011-014-9949-z |
| format | Article |
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MTT assays show that Li ion with a dosage level lower than 50 mg/l did not show detectable cytotoxicity on pheochromocytoma (PC12) cells whereas a dosage level higher than 100 mg/l resulted in significant cytotoxicity. The interaction between A[beta]^sub 42^ and Li ion occurs, and the quenching effect of Li ion on the fluorescence emission of A[Beta]^sub 42^ is found to be concentration dependent, suggesting that Li ion can bind to the A[beta]^sub 42^ molecules. CD results suggest that a more incompact conformation state will be adopted upon the interaction between A[beta]^sub 42^ and Li ion. According to AFM images, Li ion could induce the formation of the fibrils after incubation for 3 or 5 days. The formation of the oligomer and fibrils originates from the strong interactions between A[beta]^sub 42^ and Li ion. Li ion could accelerate the random coil A[beta]^sub 42^ monomers aggregating into the [beta]-sheet fibrils, which would induce the neurotoxic effect.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-014-9949-z</identifier><language>eng</language><publisher>Clifton: Springer Nature B.V</publisher><subject>Cytotoxicity ; Fluorescence ; Lithium ; Neurons ; Neurotoxicity</subject><ispartof>Biological trace element research, 2014-06, Vol.159 (1-3), p.410</ispartof><rights>Springer Science+Business Media New York 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Yang, Wang</creatorcontrib><creatorcontrib>Yan, Zhang</creatorcontrib><creatorcontrib>Hongjing, Zhang</creatorcontrib><creatorcontrib>Dexiang, Liu</creatorcontrib><creatorcontrib>Zengxun, Liu</creatorcontrib><creatorcontrib>Xiaojing, Cheng</creatorcontrib><creatorcontrib>Yi, Lu</creatorcontrib><creatorcontrib>Jichao, Jia</creatorcontrib><creatorcontrib>Jintong, Liu</creatorcontrib><creatorcontrib>Fang, Pan</creatorcontrib><title>The Toxic Effect of Lithium Ion on Neurons (PC12 cells) and A[beta]^sub 42^ Molecules</title><title>Biological trace element research</title><description>In this study, the neurotoxicity of Li ion and its effect on the morphologies of A[beta]^sub 42^ molecules were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, fluorescence (FL), atomic force microscopy (AFM), and circular dichroism (CD) spectroscopy. MTT assays show that Li ion with a dosage level lower than 50 mg/l did not show detectable cytotoxicity on pheochromocytoma (PC12) cells whereas a dosage level higher than 100 mg/l resulted in significant cytotoxicity. The interaction between A[beta]^sub 42^ and Li ion occurs, and the quenching effect of Li ion on the fluorescence emission of A[Beta]^sub 42^ is found to be concentration dependent, suggesting that Li ion can bind to the A[beta]^sub 42^ molecules. CD results suggest that a more incompact conformation state will be adopted upon the interaction between A[beta]^sub 42^ and Li ion. According to AFM images, Li ion could induce the formation of the fibrils after incubation for 3 or 5 days. The formation of the oligomer and fibrils originates from the strong interactions between A[beta]^sub 42^ and Li ion. Li ion could accelerate the random coil A[beta]^sub 42^ monomers aggregating into the [beta]-sheet fibrils, which would induce the neurotoxic effect.[PUBLICATION ABSTRACT]</description><subject>Cytotoxicity</subject><subject>Fluorescence</subject><subject>Lithium</subject><subject>Neurons</subject><subject>Neurotoxicity</subject><issn>0163-4984</issn><issn>1559-0720</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNjs1Kw0AURgepYPx5AHcXumkXo_dOJo2zlNJSQcVFXIktabyhKWNGczNQfPpm4QMUPjiLcxafUreEd4SY3wsZJNJIVjtnnf47UwllmdOYGxypBGmWause7IW6FNkjUm5cmqj3YsdQhENTwaKuueoh1PDc9LsmfsNTaGHYK8cutAKTtzkZqNh7mULZfsHjx5b78nMtcQvWrOEleK6iZ7lW53XphW_-eaXGy0UxX-mfLvxGln6zD7FrB7WhLLXW5rPh32nVEU1yRNg</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Yang, Wang</creator><creator>Yan, Zhang</creator><creator>Hongjing, Zhang</creator><creator>Dexiang, Liu</creator><creator>Zengxun, Liu</creator><creator>Xiaojing, Cheng</creator><creator>Yi, Lu</creator><creator>Jichao, Jia</creator><creator>Jintong, Liu</creator><creator>Fang, Pan</creator><general>Springer Nature B.V</general><scope>3V.</scope><scope>7QH</scope><scope>7QP</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20140601</creationdate><title>The Toxic Effect of Lithium Ion on Neurons (PC12 cells) and A[beta]^sub 42^ Molecules</title><author>Yang, Wang ; 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MTT assays show that Li ion with a dosage level lower than 50 mg/l did not show detectable cytotoxicity on pheochromocytoma (PC12) cells whereas a dosage level higher than 100 mg/l resulted in significant cytotoxicity. The interaction between A[beta]^sub 42^ and Li ion occurs, and the quenching effect of Li ion on the fluorescence emission of A[Beta]^sub 42^ is found to be concentration dependent, suggesting that Li ion can bind to the A[beta]^sub 42^ molecules. CD results suggest that a more incompact conformation state will be adopted upon the interaction between A[beta]^sub 42^ and Li ion. According to AFM images, Li ion could induce the formation of the fibrils after incubation for 3 or 5 days. The formation of the oligomer and fibrils originates from the strong interactions between A[beta]^sub 42^ and Li ion. Li ion could accelerate the random coil A[beta]^sub 42^ monomers aggregating into the [beta]-sheet fibrils, which would induce the neurotoxic effect.[PUBLICATION ABSTRACT]</abstract><cop>Clifton</cop><pub>Springer Nature B.V</pub><doi>10.1007/s12011-014-9949-z</doi></addata></record> |
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| subjects | Cytotoxicity Fluorescence Lithium Neurons Neurotoxicity |
| title | The Toxic Effect of Lithium Ion on Neurons (PC12 cells) and A[beta]^sub 42^ Molecules |
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