Gene microarray analysis of CD4-positive T cells in chronic rhinosinusitis (CRS)

Gene microarray analysis of CD4-positive T cells in chronic rhinosinusitis (CRS)

CRS is a common chronic disease, though knowledge of its pathophysiology is limited. CD4-positive T cells regulate a variety of inflammatory processes, infiltrate the sinus mucosa in CRS, and may play a pivotal role in the disease process. We analyzed gene transcription in peripheral blood CD4-posit...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of allergy and clinical immunology 2004-02, Vol.113 (2), p.S332-S332
Hauptverfasser: Davis, L.J.B., Ponikau, J., Sherris, D.A., Plager, D.A., Kita, H.
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page S332
container_issue 2
container_start_page S332
container_title Journal of allergy and clinical immunology
container_volume 113
creator Davis, L.J.B.
Ponikau, J.
Sherris, D.A.
Plager, D.A.
Kita, H.
description CRS is a common chronic disease, though knowledge of its pathophysiology is limited. CD4-positive T cells regulate a variety of inflammatory processes, infiltrate the sinus mucosa in CRS, and may play a pivotal role in the disease process. We analyzed gene transcription in peripheral blood CD4-positive T cells purified from 3 CRS patients using the Affymetrix U133A GeneChip microarray. Three allergic rhinitis patients (AR), and 4 normal individuals (NC) were controls. Levels of gene transcription were normalized and compared statistically. CRS patients demonstrated >3 months sinus symptoms, negative skin prick tests, and mucosal thickening by rhinoscopy and CT scan. Among 14,036 genes analyzed, 43 transcripts were uniquely (not in AR or NC) and significantly (p
doi_str_mv 10.1016/j.jaci.2004.01.702
format Article
fullrecord <record><control><sourceid>proquest_elsev</sourceid><recordid>TN_cdi_proquest_journals_1504858603</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0091674904007419</els_id><sourcerecordid>3239719211</sourcerecordid><originalsourceid>FETCH-LOGICAL-e813-4c30d2a15799d17f0d2e01b615725a4991ed58a699d844f465a63b1458cbb0313</originalsourceid><addsrcrecordid>eNotkF9LwzAUxYMoOKdfwKeAL_rQem_zpyn4IlWnMFB07yFNM5ZS25lsg317U_Tpcu45HA4_Qq4RcgSU913eGevzAoDngHkJxQmZIVRlJlUhTskMoMJMlrw6JxcxdpA0U9WMfCzc4Oi3t2E0IZgjNYPpj9FHOq5p_cSz7Rj9zh8cXVHr-j5SP1C7CePgLQ0bPyR72E-RSG_rz6-7S3K2Nn10V_93TlYvz6v6NVu-L97qx2XmFLKMWwZtYVCUVdViuU7CATYyPQpheFWha4UyMrmK8zWXwkjWIBfKNg0wZHNy81e7DePP3sWd7sZ9SNujRgFcCSWBpdTDX8qlJQfvgo7Wu8G61gdnd7odvUbQE0Ld6QmhnhBqQJ0Qsl_4omQu</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1504858603</pqid></control><display><type>article</type><title>Gene microarray analysis of CD4-positive T cells in chronic rhinosinusitis (CRS)</title><source>Elsevier ScienceDirect Journals Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Davis, L.J.B. ; Ponikau, J. ; Sherris, D.A. ; Plager, D.A. ; Kita, H.</creator><creatorcontrib>Davis, L.J.B. ; Ponikau, J. ; Sherris, D.A. ; Plager, D.A. ; Kita, H.</creatorcontrib><description>CRS is a common chronic disease, though knowledge of its pathophysiology is limited. CD4-positive T cells regulate a variety of inflammatory processes, infiltrate the sinus mucosa in CRS, and may play a pivotal role in the disease process. We analyzed gene transcription in peripheral blood CD4-positive T cells purified from 3 CRS patients using the Affymetrix U133A GeneChip microarray. Three allergic rhinitis patients (AR), and 4 normal individuals (NC) were controls. Levels of gene transcription were normalized and compared statistically. CRS patients demonstrated &gt;3 months sinus symptoms, negative skin prick tests, and mucosal thickening by rhinoscopy and CT scan. Among 14,036 genes analyzed, 43 transcripts were uniquely (not in AR or NC) and significantly (p&lt;0.05) increased greater than 2-fold in patients with CRS; 11 transcripts decreased at least 0.5-fold in CRS. The increased transcripts encode transcription factors (14), signaling molecules (5), cell surface molecules (3), cytoplasmic proteins (6), enzymes (6), inflammatory mediators (2), and proteins with unknown function (7). For example, an MHC Class I-like molecule, CD1b, mRNA expression in CD4-positive T-cells in CRS exhibited 3.59–fold greater expression than AR (p=.04) and 3.78–fold greater expression than NC (p=.01). An NK cell receptor, KIR-123FM, had 3.49–fold higher expression in CRS compared to AR (p=.03) and a 2.15-fold increase compared to NC (P=.04). CD4-positive T cells from patients with CRS express unique sets of genes compared to those from NC or AR. Dysregulated gene expression and subsequent functional alteration in T cells may be implicated in the pathogenesis of CRS.</description><identifier>ISSN: 0091-6749</identifier><identifier>EISSN: 1097-6825</identifier><identifier>DOI: 10.1016/j.jaci.2004.01.702</identifier><language>eng</language><publisher>St. Louis: Mosby, Inc</publisher><ispartof>Journal of allergy and clinical immunology, 2004-02, Vol.113 (2), p.S332-S332</ispartof><rights>2004</rights><rights>Copyright Elsevier Limited Feb 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jaci.2004.01.702$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Davis, L.J.B.</creatorcontrib><creatorcontrib>Ponikau, J.</creatorcontrib><creatorcontrib>Sherris, D.A.</creatorcontrib><creatorcontrib>Plager, D.A.</creatorcontrib><creatorcontrib>Kita, H.</creatorcontrib><title>Gene microarray analysis of CD4-positive T cells in chronic rhinosinusitis (CRS)</title><title>Journal of allergy and clinical immunology</title><description>CRS is a common chronic disease, though knowledge of its pathophysiology is limited. CD4-positive T cells regulate a variety of inflammatory processes, infiltrate the sinus mucosa in CRS, and may play a pivotal role in the disease process. We analyzed gene transcription in peripheral blood CD4-positive T cells purified from 3 CRS patients using the Affymetrix U133A GeneChip microarray. Three allergic rhinitis patients (AR), and 4 normal individuals (NC) were controls. Levels of gene transcription were normalized and compared statistically. CRS patients demonstrated &gt;3 months sinus symptoms, negative skin prick tests, and mucosal thickening by rhinoscopy and CT scan. Among 14,036 genes analyzed, 43 transcripts were uniquely (not in AR or NC) and significantly (p&lt;0.05) increased greater than 2-fold in patients with CRS; 11 transcripts decreased at least 0.5-fold in CRS. The increased transcripts encode transcription factors (14), signaling molecules (5), cell surface molecules (3), cytoplasmic proteins (6), enzymes (6), inflammatory mediators (2), and proteins with unknown function (7). For example, an MHC Class I-like molecule, CD1b, mRNA expression in CD4-positive T-cells in CRS exhibited 3.59–fold greater expression than AR (p=.04) and 3.78–fold greater expression than NC (p=.01). An NK cell receptor, KIR-123FM, had 3.49–fold higher expression in CRS compared to AR (p=.03) and a 2.15-fold increase compared to NC (P=.04). CD4-positive T cells from patients with CRS express unique sets of genes compared to those from NC or AR. Dysregulated gene expression and subsequent functional alteration in T cells may be implicated in the pathogenesis of CRS.</description><issn>0091-6749</issn><issn>1097-6825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNotkF9LwzAUxYMoOKdfwKeAL_rQem_zpyn4IlWnMFB07yFNM5ZS25lsg317U_Tpcu45HA4_Qq4RcgSU913eGevzAoDngHkJxQmZIVRlJlUhTskMoMJMlrw6JxcxdpA0U9WMfCzc4Oi3t2E0IZgjNYPpj9FHOq5p_cSz7Rj9zh8cXVHr-j5SP1C7CePgLQ0bPyR72E-RSG_rz6-7S3K2Nn10V_93TlYvz6v6NVu-L97qx2XmFLKMWwZtYVCUVdViuU7CATYyPQpheFWha4UyMrmK8zWXwkjWIBfKNg0wZHNy81e7DePP3sWd7sZ9SNujRgFcCSWBpdTDX8qlJQfvgo7Wu8G61gdnd7odvUbQE0Ld6QmhnhBqQJ0Qsl_4omQu</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Davis, L.J.B.</creator><creator>Ponikau, J.</creator><creator>Sherris, D.A.</creator><creator>Plager, D.A.</creator><creator>Kita, H.</creator><general>Mosby, Inc</general><general>Elsevier Limited</general><scope>7SS</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope></search><sort><creationdate>20040201</creationdate><title>Gene microarray analysis of CD4-positive T cells in chronic rhinosinusitis (CRS)</title><author>Davis, L.J.B. ; Ponikau, J. ; Sherris, D.A. ; Plager, D.A. ; Kita, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e813-4c30d2a15799d17f0d2e01b615725a4991ed58a699d844f465a63b1458cbb0313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Davis, L.J.B.</creatorcontrib><creatorcontrib>Ponikau, J.</creatorcontrib><creatorcontrib>Sherris, D.A.</creatorcontrib><creatorcontrib>Plager, D.A.</creatorcontrib><creatorcontrib>Kita, H.</creatorcontrib><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Nursing &amp; Allied Health Premium</collection><jtitle>Journal of allergy and clinical immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Davis, L.J.B.</au><au>Ponikau, J.</au><au>Sherris, D.A.</au><au>Plager, D.A.</au><au>Kita, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene microarray analysis of CD4-positive T cells in chronic rhinosinusitis (CRS)</atitle><jtitle>Journal of allergy and clinical immunology</jtitle><date>2004-02-01</date><risdate>2004</risdate><volume>113</volume><issue>2</issue><spage>S332</spage><epage>S332</epage><pages>S332-S332</pages><issn>0091-6749</issn><eissn>1097-6825</eissn><abstract>CRS is a common chronic disease, though knowledge of its pathophysiology is limited. CD4-positive T cells regulate a variety of inflammatory processes, infiltrate the sinus mucosa in CRS, and may play a pivotal role in the disease process. We analyzed gene transcription in peripheral blood CD4-positive T cells purified from 3 CRS patients using the Affymetrix U133A GeneChip microarray. Three allergic rhinitis patients (AR), and 4 normal individuals (NC) were controls. Levels of gene transcription were normalized and compared statistically. CRS patients demonstrated &gt;3 months sinus symptoms, negative skin prick tests, and mucosal thickening by rhinoscopy and CT scan. Among 14,036 genes analyzed, 43 transcripts were uniquely (not in AR or NC) and significantly (p&lt;0.05) increased greater than 2-fold in patients with CRS; 11 transcripts decreased at least 0.5-fold in CRS. The increased transcripts encode transcription factors (14), signaling molecules (5), cell surface molecules (3), cytoplasmic proteins (6), enzymes (6), inflammatory mediators (2), and proteins with unknown function (7). For example, an MHC Class I-like molecule, CD1b, mRNA expression in CD4-positive T-cells in CRS exhibited 3.59–fold greater expression than AR (p=.04) and 3.78–fold greater expression than NC (p=.01). An NK cell receptor, KIR-123FM, had 3.49–fold higher expression in CRS compared to AR (p=.03) and a 2.15-fold increase compared to NC (P=.04). CD4-positive T cells from patients with CRS express unique sets of genes compared to those from NC or AR. Dysregulated gene expression and subsequent functional alteration in T cells may be implicated in the pathogenesis of CRS.</abstract><cop>St. Louis</cop><pub>Mosby, Inc</pub><doi>10.1016/j.jaci.2004.01.702</doi></addata></record>
fulltext fulltext
identifier ISSN: 0091-6749
ispartof Journal of allergy and clinical immunology, 2004-02, Vol.113 (2), p.S332-S332
issn 0091-6749
1097-6825
language eng
recordid cdi_proquest_journals_1504858603
source Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
title Gene microarray analysis of CD4-positive T cells in chronic rhinosinusitis (CRS)
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T12%3A23%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_elsev&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Gene%20microarray%20analysis%20of%20CD4-positive%20T%20cells%20in%20chronic%20rhinosinusitis%20(CRS)&rft.jtitle=Journal%20of%20allergy%20and%20clinical%20immunology&rft.au=Davis,%20L.J.B.&rft.date=2004-02-01&rft.volume=113&rft.issue=2&rft.spage=S332&rft.epage=S332&rft.pages=S332-S332&rft.issn=0091-6749&rft.eissn=1097-6825&rft_id=info:doi/10.1016/j.jaci.2004.01.702&rft_dat=%3Cproquest_elsev%3E3239719211%3C/proquest_elsev%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1504858603&rft_id=info:pmid/&rft_els_id=S0091674904007419&rfr_iscdi=true