Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum
Phaeodactylum tricornutum is a diatom that is rich in lipids. Recently, it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral lipids （mainly triacylglycerides, or TAGs） of microalgae, including P. tricornutum, b...
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| Veröffentlicht in: | Chinese journal of oceanology and limnology 2014-03, Vol.32 (2), p.397-402 |
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| creator | 张琳 韩吉昌 杨官品 朱葆华 潘克厚 |
| description | Phaeodactylum tricornutum is a diatom that is rich in lipids. Recently, it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral lipids （mainly triacylglycerides, or TAGs） of microalgae, including P. tricornutum, but the mechanism is unclear. In this study, we deciphered the correlations between TAG content and nine key enzymatic genes involved in lipid synthesis in P. tricornuturn. After being cultured under nitrogen-free conditions for 0, 4, 24, 48, 72, 120, and 168 h, the TAG contents ofP. tricornutum cells were assayed and the transcript abundances of the target genes were monitored by quantitative real-time PCR. The results show that the abundances of four target gene transcripts （LACS3, G3PDH2, G3PDH3, and G3PDH5） were positively correlated with TAG content, indicating that these genes may be involved in TAG synthesis in P. tricornutum. The findings improve our understanding of the metabolic network and regulation of lipid synthesis and will guide the future genetic improvement of the TAG content ofP. tricornutum. |
| doi_str_mv | 10.1007/s00343-014-3173-8 |
| format | Article |
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Recently, it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral lipids （mainly triacylglycerides, or TAGs） of microalgae, including P. tricornutum, but the mechanism is unclear. In this study, we deciphered the correlations between TAG content and nine key enzymatic genes involved in lipid synthesis in P. tricornuturn. After being cultured under nitrogen-free conditions for 0, 4, 24, 48, 72, 120, and 168 h, the TAG contents ofP. tricornutum cells were assayed and the transcript abundances of the target genes were monitored by quantitative real-time PCR. The results show that the abundances of four target gene transcripts （LACS3, G3PDH2, G3PDH3, and G3PDH5） were positively correlated with TAG content, indicating that these genes may be involved in TAG synthesis in P. tricornutum. The findings improve our understanding of the metabolic network and regulation of lipid synthesis and will guide the future genetic improvement of the TAG content ofP. tricornutum.</description><identifier>ISSN: 0254-4059</identifier><identifier>ISSN: 2096-5508</identifier><identifier>EISSN: 1993-5005</identifier><identifier>EISSN: 2523-3521</identifier><identifier>DOI: 10.1007/s00343-014-3173-8</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Bacillariophyceae ; biodiesel ; Biodiesel fuels ; Biofuels ; Biology ; Chemical synthesis ; correlation ; Diatoms ; Earth and Environmental Science ; Earth Sciences ; feedstocks ; Genes ; genetic improvement ; Lipids ; Marine ; messenger RNA ; Metabolic networks ; microalgae ; Nitrogen ; Nucleotide sequence ; Oceanography ; PCR ; Phaeodactylum tricornutum ; Phytoplankton ; quantitative polymerase chain reaction ; Synthesis ; triacylglycerols ; Triglycerides ; 三角褐指藻 ; 关键酶基因 ; 协会 ; 甘油酯 ; 缺氮 ; 脂质合成 ; 转录本 ; 酯含量</subject><ispartof>Chinese journal of oceanology and limnology, 2014-03, Vol.32 (2), p.397-402</ispartof><rights>Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2014</rights><rights>Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2014.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-1c87d9c251fcc419e201557a716d70b1dacdf7d52cdd6c54834f3583a2f70bb73</citedby><cites>FETCH-LOGICAL-c399t-1c87d9c251fcc419e201557a716d70b1dacdf7d52cdd6c54834f3583a2f70bb73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84119X/84119X.jpg</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1504359874/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1504359874?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,21367,27901,27902,33721,43781,74045</link.rule.ids></links><search><creatorcontrib>张琳 韩吉昌 杨官品 朱葆华 潘克厚</creatorcontrib><title>Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum</title><title>Chinese journal of oceanology and limnology</title><addtitle>Chin. J. Ocean. Limnol</addtitle><addtitle>Chinese Journal of Oceanology and Limnology</addtitle><description>Phaeodactylum tricornutum is a diatom that is rich in lipids. Recently, it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral lipids （mainly triacylglycerides, or TAGs） of microalgae, including P. tricornutum, but the mechanism is unclear. In this study, we deciphered the correlations between TAG content and nine key enzymatic genes involved in lipid synthesis in P. tricornuturn. After being cultured under nitrogen-free conditions for 0, 4, 24, 48, 72, 120, and 168 h, the TAG contents ofP. tricornutum cells were assayed and the transcript abundances of the target genes were monitored by quantitative real-time PCR. The results show that the abundances of four target gene transcripts （LACS3, G3PDH2, G3PDH3, and G3PDH5） were positively correlated with TAG content, indicating that these genes may be involved in TAG synthesis in P. tricornutum. The findings improve our understanding of the metabolic network and regulation of lipid synthesis and will guide the future genetic improvement of the TAG content ofP. tricornutum.</description><subject>Bacillariophyceae</subject><subject>biodiesel</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Biology</subject><subject>Chemical synthesis</subject><subject>correlation</subject><subject>Diatoms</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>feedstocks</subject><subject>Genes</subject><subject>genetic improvement</subject><subject>Lipids</subject><subject>Marine</subject><subject>messenger RNA</subject><subject>Metabolic networks</subject><subject>microalgae</subject><subject>Nitrogen</subject><subject>Nucleotide sequence</subject><subject>Oceanography</subject><subject>PCR</subject><subject>Phaeodactylum tricornutum</subject><subject>Phytoplankton</subject><subject>quantitative polymerase chain reaction</subject><subject>Synthesis</subject><subject>triacylglycerols</subject><subject>Triglycerides</subject><subject>三角褐指藻</subject><subject>关键酶基因</subject><subject>协会</subject><subject>甘油酯</subject><subject>缺氮</subject><subject>脂质合成</subject><subject>转录本</subject><subject>酯含量</subject><issn>0254-4059</issn><issn>2096-5508</issn><issn>1993-5005</issn><issn>2523-3521</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc2KFDEUhYMo2I4-gCtL3LgpTSpJJ1kOw_gDAwo665DOT3WG6qQnNzVQL-LzmqIGERcuQhLud05OOAi9JvgDwVh8BIwpoz0mrKdE0F4-QTuiFO05xvwp2uGBs55hrp6jFwB3jVYMqx36dQmQbTQ15tTl0NUSjV2mcVqsL9H5zuZUfaqdSa4NTQJb4rldD3NyJlm_ikafPHQxPeTpIaaxnbopnqPrYEn16CHCSqVYS25o53yINq6m34_GZ2dsXab5tL5tc0lznU8v0bNgJvCvHvcLdPvp-ufVl_7m2-evV5c3vaVK1Z5YKZyyAyfBWkaUHzDhXBhB9k7gA2nWLgjHB-vc3nImKQuUS2qG0MYHQS_Q-833XPL97KHqUwTrp8kkn2fQhGMpuOCcN_TdP-hdnktq6VaKUa6kYI0iG2VLBig-6HOJJ1MWTbBem9JbU7o1pdemtGyaYdNAY9Poy1_O_xG92UTBZG3GEkHf_mjfZ7gtMVDaiLePUY45jffN-U8WpjChci_pbws0rnc</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>张琳 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of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum</title><author>张琳 韩吉昌 杨官品 朱葆华 潘克厚</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-1c87d9c251fcc419e201557a716d70b1dacdf7d52cdd6c54834f3583a2f70bb73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bacillariophyceae</topic><topic>biodiesel</topic><topic>Biodiesel fuels</topic><topic>Biofuels</topic><topic>Biology</topic><topic>Chemical synthesis</topic><topic>correlation</topic><topic>Diatoms</topic><topic>Earth and Environmental Science</topic><topic>Earth Sciences</topic><topic>feedstocks</topic><topic>Genes</topic><topic>genetic improvement</topic><topic>Lipids</topic><topic>Marine</topic><topic>messenger RNA</topic><topic>Metabolic networks</topic><topic>microalgae</topic><topic>Nitrogen</topic><topic>Nucleotide 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J. Ocean. Limnol</stitle><addtitle>Chinese Journal of Oceanology and Limnology</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>32</volume><issue>2</issue><spage>397</spage><epage>402</epage><pages>397-402</pages><issn>0254-4059</issn><issn>2096-5508</issn><eissn>1993-5005</eissn><eissn>2523-3521</eissn><abstract>Phaeodactylum tricornutum is a diatom that is rich in lipids. Recently, it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral lipids （mainly triacylglycerides, or TAGs） of microalgae, including P. tricornutum, but the mechanism is unclear. In this study, we deciphered the correlations between TAG content and nine key enzymatic genes involved in lipid synthesis in P. tricornuturn. After being cultured under nitrogen-free conditions for 0, 4, 24, 48, 72, 120, and 168 h, the TAG contents ofP. tricornutum cells were assayed and the transcript abundances of the target genes were monitored by quantitative real-time PCR. The results show that the abundances of four target gene transcripts （LACS3, G3PDH2, G3PDH3, and G3PDH5） were positively correlated with TAG content, indicating that these genes may be involved in TAG synthesis in P. tricornutum. The findings improve our understanding of the metabolic network and regulation of lipid synthesis and will guide the future genetic improvement of the TAG content ofP. tricornutum.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s00343-014-3173-8</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0254-4059 |
| ispartof | Chinese journal of oceanology and limnology, 2014-03, Vol.32 (2), p.397-402 |
| issn | 0254-4059 2096-5508 1993-5005 2523-3521 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals; Alma/SFX Local Collection; ProQuest Central |
| subjects | Bacillariophyceae biodiesel Biodiesel fuels Biofuels Biology Chemical synthesis correlation Diatoms Earth and Environmental Science Earth Sciences feedstocks Genes genetic improvement Lipids Marine messenger RNA Metabolic networks microalgae Nitrogen Nucleotide sequence Oceanography PCR Phaeodactylum tricornutum Phytoplankton quantitative polymerase chain reaction Synthesis triacylglycerols Triglycerides 三角褐指藻 关键酶基因 协会 甘油酯 缺氮 脂质合成 转录本 酯含量 |
| title | Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum |
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