DOCK2 Is Essential for Antigen-Induced Translocation of TCR and Lipid Rafts, but Not PKC-[theta] and LFA-1, in T Cells
DOCK2 is a mammalian homolog ofCaenorhabditis elegansCED-5 andDrosophila melanogasterMyoblast City which are known to regulate actin cytoskeleton. DOCK2 is critical for lymphocyte migration, yet the role of DOCK2 in TCR signaling remains unclear. We show here that DOCK2 is essential for TCR-mediated...
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| Veröffentlicht in: | Immunity (Cambridge, Mass.) Mass.), 2003-07, Vol.19 (1), p.119 |
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| creator | Sanui, Terukazu Inayoshi, Ayumi Noda, Mayuko Iwata, Eiko Oike, Masahiro Sasazuki, Takehiko Fukui, Yoshinori |
| description | DOCK2 is a mammalian homolog ofCaenorhabditis elegansCED-5 andDrosophila melanogasterMyoblast City which are known to regulate actin cytoskeleton. DOCK2 is critical for lymphocyte migration, yet the role of DOCK2 in TCR signaling remains unclear. We show here that DOCK2 is essential for TCR-mediated Rac activation and immunological synapse formation. In DOCK2-deficient T cells, antigen-induced translocation of TCR and lipid rafts, but not PKC-θ and LFA-1, to the APC interface was severely impaired, resulting in a significant reduction of antigen-specific T cell proliferation. In addition, we found that the efficacy of both positive and negative selection was reduced in DOCK2-deficient mice. These results suggest that DOCK2 regulates T cell responsiveness through remodeling of actin cytoskeleton via Rac activation. |
| doi_str_mv | 10.1016/S1074-7613(03)00169-9 |
| format | Article |
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| ispartof | Immunity (Cambridge, Mass.), 2003-07, Vol.19 (1), p.119 |
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| source | Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via ScienceDirect (Elsevier) |
| subjects | Apoptosis Cell growth Chemokines Cytoskeleton Kinases Lipids Lymphocytes Microscopy Peptides Phosphorylation Proteins Rodents T cell receptors |
| title | DOCK2 Is Essential for Antigen-Induced Translocation of TCR and Lipid Rafts, but Not PKC-[theta] and LFA-1, in T Cells |
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