Calcium-induced calcium release mediates all-or-nothing responses of mesenchymal stromal cells to noradrenaline
By using Ca 2+ imaging and Fluo-4 dye, we examined the capability of certain agonists of G-protein coupled receptors to stimulate Ca 2+ signaling in cultured mesenchymal stromal cells (MSC) derived from the human adipose tissue. In particular, a small subpopulation (∼5%) MSC was found to respond to...
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| Veröffentlicht in: | Biochemistry (Moscow). Supplement series A, Membrane and cell biology Membrane and cell biology, 2014, Vol.8 (1), p.82-88 |
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| container_title | Biochemistry (Moscow). Supplement series A, Membrane and cell biology |
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| creator | Kotova, P. D. Turin-Kuzmin, P. A. Rogachevskaja, O. A. Fadeeva, J. I. Sysoeva, V. Yu Tkachuk, V. A. Kolesnikov, S. S. |
| description | By using Ca
2+
imaging and Fluo-4 dye, we examined the capability of certain agonists of G-protein coupled receptors to stimulate Ca
2+
signaling in cultured mesenchymal stromal cells (MSC) derived from the human adipose tissue. In particular, a small subpopulation (∼5%) MSC was found to respond to noradrenaline with Ca
2+
transients. The all-or-nothing fashion was characteristic of adrenergic Ca
2+
signaling in MSC, that is, while at low concentrations noradrenaline stimulated undetectable Ca
2+
transients, virtually maximal responses were elicited by this agonist at any concentration above the threshold of 100–200 nM. In some experiments, MSC were loaded with the photosensitive Ca
2+
chelator NP-EGTA to produce local or global jumps in cytosolic Ca
2+
concentration by virtue of Ca
2+
uncaging. Global uncaging eliciting a high enough Ca
2+
jump triggered a Ca
2+
transient in the MSC cytoplasm, which was similar to a noradrenaline response kinetically and by magnitude. When Ca
2+
uncaging was produced locally, it initiated a Ca
2+
signal that traveled along a cell with a speed that exceeded an expected one by two orders of magnitude, should Ca
2+
signal transfer be mediated merely by passive Ca
2+
diffusion in the presence of Ca
2+
buffer. These findings implicated Ca
2+
-induced Ca
2+
release (CICR) as a mechanism amplifying local Ca
2+
signals in MSC. Of Ca
2+
targets involved in CICR, the ryanodine receptor and IP
3
receptor are only known. The inhibitory analysis revealed IP
3
receptors to be principally responsible for CICR in MSC, whereas a contribution of ryanodine receptors was negligible. Altogether, our results suggest that an initial noradrenaline-dependent rise in cytosolic Ca
2+
stimulates, should it reach the threshold level, IP
3
receptors, thereby triggering an avalanche-like Ca
2+
release from Ca
2+
stores and underlying the all-or-nothing dependence of cellular responses on the agonist concentration. |
| doi_str_mv | 10.1134/S1990747813050085 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1501535262</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3228165181</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-621e3584823ad14eae2e8f8ad15853af76bdfc42dee3c351ccb821f9aa1f408e3</originalsourceid><addsrcrecordid>eNp1UEtLAzEQDqJgrf4AbwueVzN57GaPUnxBwYN6XtLspN2STWqye-i_N6Uignj6ZuZ7MHyEXAO9BeDi7g2ahtaiVsCppFTJEzI7nMpaNOL0Z67VOblIaUtpxUVVzUhYaGf6aSh7300Gu8Ic9yKiQ52wGLDr9Yip0M6VIZY-jJverzOfdsGnTASbRQm92ewH7Yo0xnBAg86lYgyFD1F3Eb12vcdLcma1S3j1jXPy8fjwvngul69PL4v7ZWk4VGNZMUAulVCM6w4EamSorMqzVJJrW1erzhrBOkRuuARjVoqBbbQGK6hCPic3x9xdDJ8TprHdhinmH1ILkoLkklUsq-CoMjGkFNG2u9gPOu5boO2h1_ZPr9nDjp6UtX6N8Vfyv6YvPH18XA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1501535262</pqid></control><display><type>article</type><title>Calcium-induced calcium release mediates all-or-nothing responses of mesenchymal stromal cells to noradrenaline</title><source>SpringerLink Journals - AutoHoldings</source><creator>Kotova, P. D. ; Turin-Kuzmin, P. A. ; Rogachevskaja, O. A. ; Fadeeva, J. I. ; Sysoeva, V. Yu ; Tkachuk, V. A. ; Kolesnikov, S. S.</creator><creatorcontrib>Kotova, P. D. ; Turin-Kuzmin, P. A. ; Rogachevskaja, O. A. ; Fadeeva, J. I. ; Sysoeva, V. Yu ; Tkachuk, V. A. ; Kolesnikov, S. S.</creatorcontrib><description>By using Ca
2+
imaging and Fluo-4 dye, we examined the capability of certain agonists of G-protein coupled receptors to stimulate Ca
2+
signaling in cultured mesenchymal stromal cells (MSC) derived from the human adipose tissue. In particular, a small subpopulation (∼5%) MSC was found to respond to noradrenaline with Ca
2+
transients. The all-or-nothing fashion was characteristic of adrenergic Ca
2+
signaling in MSC, that is, while at low concentrations noradrenaline stimulated undetectable Ca
2+
transients, virtually maximal responses were elicited by this agonist at any concentration above the threshold of 100–200 nM. In some experiments, MSC were loaded with the photosensitive Ca
2+
chelator NP-EGTA to produce local or global jumps in cytosolic Ca
2+
concentration by virtue of Ca
2+
uncaging. Global uncaging eliciting a high enough Ca
2+
jump triggered a Ca
2+
transient in the MSC cytoplasm, which was similar to a noradrenaline response kinetically and by magnitude. When Ca
2+
uncaging was produced locally, it initiated a Ca
2+
signal that traveled along a cell with a speed that exceeded an expected one by two orders of magnitude, should Ca
2+
signal transfer be mediated merely by passive Ca
2+
diffusion in the presence of Ca
2+
buffer. These findings implicated Ca
2+
-induced Ca
2+
release (CICR) as a mechanism amplifying local Ca
2+
signals in MSC. Of Ca
2+
targets involved in CICR, the ryanodine receptor and IP
3
receptor are only known. The inhibitory analysis revealed IP
3
receptors to be principally responsible for CICR in MSC, whereas a contribution of ryanodine receptors was negligible. Altogether, our results suggest that an initial noradrenaline-dependent rise in cytosolic Ca
2+
stimulates, should it reach the threshold level, IP
3
receptors, thereby triggering an avalanche-like Ca
2+
release from Ca
2+
stores and underlying the all-or-nothing dependence of cellular responses on the agonist concentration.</description><identifier>ISSN: 1990-7478</identifier><identifier>EISSN: 1990-7494</identifier><identifier>DOI: 10.1134/S1990747813050085</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biomedical and Life Sciences ; Calcium ; Cell Biology ; Life Sciences ; Proteins ; Signal transduction ; Stem cells</subject><ispartof>Biochemistry (Moscow). Supplement series A, Membrane and cell biology, 2014, Vol.8 (1), p.82-88</ispartof><rights>Pleiades Publishing, Ltd. 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-621e3584823ad14eae2e8f8ad15853af76bdfc42dee3c351ccb821f9aa1f408e3</citedby><cites>FETCH-LOGICAL-c316t-621e3584823ad14eae2e8f8ad15853af76bdfc42dee3c351ccb821f9aa1f408e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1990747813050085$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1990747813050085$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27913,27914,41477,42546,51308</link.rule.ids></links><search><creatorcontrib>Kotova, P. D.</creatorcontrib><creatorcontrib>Turin-Kuzmin, P. A.</creatorcontrib><creatorcontrib>Rogachevskaja, O. A.</creatorcontrib><creatorcontrib>Fadeeva, J. I.</creatorcontrib><creatorcontrib>Sysoeva, V. Yu</creatorcontrib><creatorcontrib>Tkachuk, V. A.</creatorcontrib><creatorcontrib>Kolesnikov, S. S.</creatorcontrib><title>Calcium-induced calcium release mediates all-or-nothing responses of mesenchymal stromal cells to noradrenaline</title><title>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</title><addtitle>Biochem. Moscow Suppl. Ser. A</addtitle><description>By using Ca
2+
imaging and Fluo-4 dye, we examined the capability of certain agonists of G-protein coupled receptors to stimulate Ca
2+
signaling in cultured mesenchymal stromal cells (MSC) derived from the human adipose tissue. In particular, a small subpopulation (∼5%) MSC was found to respond to noradrenaline with Ca
2+
transients. The all-or-nothing fashion was characteristic of adrenergic Ca
2+
signaling in MSC, that is, while at low concentrations noradrenaline stimulated undetectable Ca
2+
transients, virtually maximal responses were elicited by this agonist at any concentration above the threshold of 100–200 nM. In some experiments, MSC were loaded with the photosensitive Ca
2+
chelator NP-EGTA to produce local or global jumps in cytosolic Ca
2+
concentration by virtue of Ca
2+
uncaging. Global uncaging eliciting a high enough Ca
2+
jump triggered a Ca
2+
transient in the MSC cytoplasm, which was similar to a noradrenaline response kinetically and by magnitude. When Ca
2+
uncaging was produced locally, it initiated a Ca
2+
signal that traveled along a cell with a speed that exceeded an expected one by two orders of magnitude, should Ca
2+
signal transfer be mediated merely by passive Ca
2+
diffusion in the presence of Ca
2+
buffer. These findings implicated Ca
2+
-induced Ca
2+
release (CICR) as a mechanism amplifying local Ca
2+
signals in MSC. Of Ca
2+
targets involved in CICR, the ryanodine receptor and IP
3
receptor are only known. The inhibitory analysis revealed IP
3
receptors to be principally responsible for CICR in MSC, whereas a contribution of ryanodine receptors was negligible. Altogether, our results suggest that an initial noradrenaline-dependent rise in cytosolic Ca
2+
stimulates, should it reach the threshold level, IP
3
receptors, thereby triggering an avalanche-like Ca
2+
release from Ca
2+
stores and underlying the all-or-nothing dependence of cellular responses on the agonist concentration.</description><subject>Biomedical and Life Sciences</subject><subject>Calcium</subject><subject>Cell Biology</subject><subject>Life Sciences</subject><subject>Proteins</subject><subject>Signal transduction</subject><subject>Stem cells</subject><issn>1990-7478</issn><issn>1990-7494</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UEtLAzEQDqJgrf4AbwueVzN57GaPUnxBwYN6XtLspN2STWqye-i_N6Uignj6ZuZ7MHyEXAO9BeDi7g2ahtaiVsCppFTJEzI7nMpaNOL0Z67VOblIaUtpxUVVzUhYaGf6aSh7300Gu8Ic9yKiQ52wGLDr9Yip0M6VIZY-jJverzOfdsGnTASbRQm92ewH7Yo0xnBAg86lYgyFD1F3Eb12vcdLcma1S3j1jXPy8fjwvngul69PL4v7ZWk4VGNZMUAulVCM6w4EamSorMqzVJJrW1erzhrBOkRuuARjVoqBbbQGK6hCPic3x9xdDJ8TprHdhinmH1ILkoLkklUsq-CoMjGkFNG2u9gPOu5boO2h1_ZPr9nDjp6UtX6N8Vfyv6YvPH18XA</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Kotova, P. D.</creator><creator>Turin-Kuzmin, P. A.</creator><creator>Rogachevskaja, O. A.</creator><creator>Fadeeva, J. I.</creator><creator>Sysoeva, V. Yu</creator><creator>Tkachuk, V. A.</creator><creator>Kolesnikov, S. S.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>2014</creationdate><title>Calcium-induced calcium release mediates all-or-nothing responses of mesenchymal stromal cells to noradrenaline</title><author>Kotova, P. D. ; Turin-Kuzmin, P. A. ; Rogachevskaja, O. A. ; Fadeeva, J. I. ; Sysoeva, V. Yu ; Tkachuk, V. A. ; Kolesnikov, S. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-621e3584823ad14eae2e8f8ad15853af76bdfc42dee3c351ccb821f9aa1f408e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biomedical and Life Sciences</topic><topic>Calcium</topic><topic>Cell Biology</topic><topic>Life Sciences</topic><topic>Proteins</topic><topic>Signal transduction</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kotova, P. D.</creatorcontrib><creatorcontrib>Turin-Kuzmin, P. A.</creatorcontrib><creatorcontrib>Rogachevskaja, O. A.</creatorcontrib><creatorcontrib>Fadeeva, J. I.</creatorcontrib><creatorcontrib>Sysoeva, V. Yu</creatorcontrib><creatorcontrib>Tkachuk, V. A.</creatorcontrib><creatorcontrib>Kolesnikov, S. S.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kotova, P. D.</au><au>Turin-Kuzmin, P. A.</au><au>Rogachevskaja, O. A.</au><au>Fadeeva, J. I.</au><au>Sysoeva, V. Yu</au><au>Tkachuk, V. A.</au><au>Kolesnikov, S. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium-induced calcium release mediates all-or-nothing responses of mesenchymal stromal cells to noradrenaline</atitle><jtitle>Biochemistry (Moscow). Supplement series A, Membrane and cell biology</jtitle><stitle>Biochem. Moscow Suppl. Ser. A</stitle><date>2014</date><risdate>2014</risdate><volume>8</volume><issue>1</issue><spage>82</spage><epage>88</epage><pages>82-88</pages><issn>1990-7478</issn><eissn>1990-7494</eissn><abstract>By using Ca
2+
imaging and Fluo-4 dye, we examined the capability of certain agonists of G-protein coupled receptors to stimulate Ca
2+
signaling in cultured mesenchymal stromal cells (MSC) derived from the human adipose tissue. In particular, a small subpopulation (∼5%) MSC was found to respond to noradrenaline with Ca
2+
transients. The all-or-nothing fashion was characteristic of adrenergic Ca
2+
signaling in MSC, that is, while at low concentrations noradrenaline stimulated undetectable Ca
2+
transients, virtually maximal responses were elicited by this agonist at any concentration above the threshold of 100–200 nM. In some experiments, MSC were loaded with the photosensitive Ca
2+
chelator NP-EGTA to produce local or global jumps in cytosolic Ca
2+
concentration by virtue of Ca
2+
uncaging. Global uncaging eliciting a high enough Ca
2+
jump triggered a Ca
2+
transient in the MSC cytoplasm, which was similar to a noradrenaline response kinetically and by magnitude. When Ca
2+
uncaging was produced locally, it initiated a Ca
2+
signal that traveled along a cell with a speed that exceeded an expected one by two orders of magnitude, should Ca
2+
signal transfer be mediated merely by passive Ca
2+
diffusion in the presence of Ca
2+
buffer. These findings implicated Ca
2+
-induced Ca
2+
release (CICR) as a mechanism amplifying local Ca
2+
signals in MSC. Of Ca
2+
targets involved in CICR, the ryanodine receptor and IP
3
receptor are only known. The inhibitory analysis revealed IP
3
receptors to be principally responsible for CICR in MSC, whereas a contribution of ryanodine receptors was negligible. Altogether, our results suggest that an initial noradrenaline-dependent rise in cytosolic Ca
2+
stimulates, should it reach the threshold level, IP
3
receptors, thereby triggering an avalanche-like Ca
2+
release from Ca
2+
stores and underlying the all-or-nothing dependence of cellular responses on the agonist concentration.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1990747813050085</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1990-7478 |
| ispartof | Biochemistry (Moscow). Supplement series A, Membrane and cell biology, 2014, Vol.8 (1), p.82-88 |
| issn | 1990-7478 1990-7494 |
| language | eng |
| recordid | cdi_proquest_journals_1501535262 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biomedical and Life Sciences Calcium Cell Biology Life Sciences Proteins Signal transduction Stem cells |
| title | Calcium-induced calcium release mediates all-or-nothing responses of mesenchymal stromal cells to noradrenaline |
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