CDKG1 protein kinase is essential for synapsis and male meiosis at high ambient temperature in Arabidopsis thaliana
The Arabidopsis cyclin-dependent kinase G (CDKG) gene defines a clade of cyclin-dependent protein kinases related to CDK10 and CDK11, as well as to the enigmatic Ph1-related kinases that are implicated in controlling homeologous chromosome pairing in wheat. Here we demonstrate that the CDKG1/CYCLINL...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2014-02, Vol.111 (6), p.2182-2187 |
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| creator | Zheng, Tao Nibau, Candida Phillips, Dylan W. Jenkins, Glyn Armstrong, Susan J. Doonan, John H. |
| description | The Arabidopsis cyclin-dependent kinase G (CDKG) gene defines a clade of cyclin-dependent protein kinases related to CDK10 and CDK11, as well as to the enigmatic Ph1-related kinases that are implicated in controlling homeologous chromosome pairing in wheat. Here we demonstrate that the CDKG1/CYCLINL complex is essential for synapsis and recombination during male meiosis. A transfer-DNA insertional mutation in the cdkg1 gene leads to a temperature-sensitive failure of meiosis in late Zygotene/Pachytene that is associated with defective formation of the synaptonemal complex, reduced bivalent formation and crossing over, and aneuploid gametes. An aphenotypic insertion in the cyclin L gene, a cognate cyclin for CDKG, strongly enhances the phenotype of cdkg 1–1 mutants, indicating that this cdk–cyclin complex is essential for male meiosis. Since CYCLINL, CDKG, and their mammalian homologs have been previously shown to affect mRNA processing, particularly alternative splicing, our observations also suggest a mechanism to explain the widespread phenomenon of thermal sensitivity in male meiosis. |
| doi_str_mv | 10.1073/pnas.1318460111 |
| format | Article |
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Here we demonstrate that the CDKG1/CYCLINL complex is essential for synapsis and recombination during male meiosis. A transfer-DNA insertional mutation in the cdkg1 gene leads to a temperature-sensitive failure of meiosis in late Zygotene/Pachytene that is associated with defective formation of the synaptonemal complex, reduced bivalent formation and crossing over, and aneuploid gametes. An aphenotypic insertion in the cyclin L gene, a cognate cyclin for CDKG, strongly enhances the phenotype of cdkg 1–1 mutants, indicating that this cdk–cyclin complex is essential for male meiosis. Since CYCLINL, CDKG, and their mammalian homologs have been previously shown to affect mRNA processing, particularly alternative splicing, our observations also suggest a mechanism to explain the widespread phenomenon of thermal sensitivity in male meiosis.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1318460111</identifier><identifier>PMID: 24469829</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>alternative splicing ; ambient temperature ; aneuploidy ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis - physiology ; Arabidopsis Proteins - physiology ; Arabidopsis thaliana ; Biological Sciences ; Cell division ; Chromosome pairing ; Chromosome Pairing - physiology ; Chromosomes ; Chromosomes, Plant ; crossing over ; cyclin-dependent kinase ; cyclins ; Flowers & plants ; Genes ; Genetic loci ; Genotype & phenotype ; germ cells ; Hot Temperature ; Kinases ; males ; mammals ; Meiosis ; messenger RNA ; Metaphase ; mutants ; Mutation ; Pachytene stage ; phenotype ; Pollen ; Polymerase Chain Reaction ; Prophase ; Protein Kinases - metabolism ; Splicing ; synaptonemal complex ; transfer DNA ; Triticum aestivum ; wheat</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2014-02, Vol.111 (6), p.2182-2187</ispartof><rights>copyright © 1993–2008 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Feb 11, 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-6725fad731be98c5c018db83ffd6f1ddca037c216a5962637fe83d442ad5f9373</citedby><cites>FETCH-LOGICAL-c556t-6725fad731be98c5c018db83ffd6f1ddca037c216a5962637fe83d442ad5f9373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/111/6.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23768837$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23768837$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24469829$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Tao</creatorcontrib><creatorcontrib>Nibau, Candida</creatorcontrib><creatorcontrib>Phillips, Dylan W.</creatorcontrib><creatorcontrib>Jenkins, Glyn</creatorcontrib><creatorcontrib>Armstrong, Susan J.</creatorcontrib><creatorcontrib>Doonan, John H.</creatorcontrib><title>CDKG1 protein kinase is essential for synapsis and male meiosis at high ambient temperature in Arabidopsis thaliana</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The Arabidopsis cyclin-dependent kinase G (CDKG) gene defines a clade of cyclin-dependent protein kinases related to CDK10 and CDK11, as well as to the enigmatic Ph1-related kinases that are implicated in controlling homeologous chromosome pairing in wheat. 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Here we demonstrate that the CDKG1/CYCLINL complex is essential for synapsis and recombination during male meiosis. A transfer-DNA insertional mutation in the cdkg1 gene leads to a temperature-sensitive failure of meiosis in late Zygotene/Pachytene that is associated with defective formation of the synaptonemal complex, reduced bivalent formation and crossing over, and aneuploid gametes. An aphenotypic insertion in the cyclin L gene, a cognate cyclin for CDKG, strongly enhances the phenotype of cdkg 1–1 mutants, indicating that this cdk–cyclin complex is essential for male meiosis. Since CYCLINL, CDKG, and their mammalian homologs have been previously shown to affect mRNA processing, particularly alternative splicing, our observations also suggest a mechanism to explain the widespread phenomenon of thermal sensitivity in male meiosis.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>24469829</pmid><doi>10.1073/pnas.1318460111</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | alternative splicing ambient temperature aneuploidy Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis - physiology Arabidopsis Proteins - physiology Arabidopsis thaliana Biological Sciences Cell division Chromosome pairing Chromosome Pairing - physiology Chromosomes Chromosomes, Plant crossing over cyclin-dependent kinase cyclins Flowers & plants Genes Genetic loci Genotype & phenotype germ cells Hot Temperature Kinases males mammals Meiosis messenger RNA Metaphase mutants Mutation Pachytene stage phenotype Pollen Polymerase Chain Reaction Prophase Protein Kinases - metabolism Splicing synaptonemal complex transfer DNA Triticum aestivum wheat |
| title | CDKG1 protein kinase is essential for synapsis and male meiosis at high ambient temperature in Arabidopsis thaliana |
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